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. 1978 Jul;36(1):160–168. doi: 10.1128/aem.36.1.160-168.1978

Electron Microscopy of the Microbial Populations Present and Their Modes of Attack on Various Cellulosic Substrates Undergoing Digestion in the Sheep Rumen

D Dinsdale 1, E Jane Morris 1,†, J S D Bacon 1
PMCID: PMC243047  PMID: 16345303

Abstract

Cotton fibers and various cell wall preparations from grass leaves and from the feces of sheep fed on dried grass were placed in the sheep rumen in bags made from 5-μm-mesh nylon cloth. After periods of from 3 to 48 h, bags were removed, and the contents were fixed, embedded, sectioned, and stained for electron microscopy. Some of the bacteria present were seen to be closely associated with the cell walls, either tunneling within them or making very close contact. Evidence was obtained for differential digestion of cell walls and of the layers within them. Distinct differences were noticed between bacterial populations attacking the more susceptible wall types and those attacking feces cell walls and cotton fibers. Among the latter, the dominant form was a long, thin rod with a typical gramnegative cell wall structure, different from that described for Bacteroides succinogenes S85 or for Butyrivibrio fibrisolvens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akin D. E., Amos H. E. Rumen bacterial degradation of forage cell walls investigated by electron microscopy. Appl Microbiol. 1975 May;29(5):692–701. doi: 10.1128/am.29.5.692-701.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Akin D. E., Burdick D., Michaels G. E. Rumen bacterial interrelationships with plant tissue during degradation revealed by transmission electron microscopy. Appl Microbiol. 1974 Jun;27(6):1149–1156. doi: 10.1128/am.27.6.1149-1156.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Akin D. E. Ultrastructure of rumen bacterial attachment to forage cell walls. Appl Environ Microbiol. 1976 Apr;31(4):562–568. doi: 10.1128/aem.31.4.562-568.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BRYANT M. P. The isolation and characteristics of a spirochete from the bovine rumen. J Bacteriol. 1952 Sep;64(3):325–335. doi: 10.1128/jb.64.3.325-335.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bacon J. S., Gordon A. H., Morris E. J. Acetyl groups in cell-wall preparations from higher plants. Biochem J. 1975 Aug;149(2):485–487. doi: 10.1042/bj1490485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bryant M. P. Nutritional requirements of the predominant rumen cellulolytic bacteria. Fed Proc. 1973 Jul;32(7):1809–1813. [PubMed] [Google Scholar]
  7. Cheng K. J., Costerton J. W. Ultrastructure of Butyrivibrio fibrisolvens: a gram-positive bacterium. J Bacteriol. 1977 Mar;129(3):1506–1512. doi: 10.1128/jb.129.3.1506-1512.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cheng K. J., Costerton J. W. Ultrastructure of cell envelopes of bacteria of the bovine rumen. Appl Microbiol. 1975 Jun;29(6):841–849. doi: 10.1128/am.29.6.841-849.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Chimwano A. M., Orskov E. R., Stewart C. S. Effect of dietary proportions of roughage and concentrate on rate of digestion of dried grass and cellulose in the rumen of sheep [proceedings]. Proc Nutr Soc. 1976 Sep;35(2):101A–102A. [PubMed] [Google Scholar]
  10. Costerton J. W., Damgaard H. N., Cheng K. J. Cell envelope morphology of rumen bacteria. J Bacteriol. 1974 Jun;118(3):1132–1143. doi: 10.1128/jb.118.3.1132-1143.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HALLIWELL G., BRYANT M. P. THE CELLULOLYTIC ACTIVITY OF PURE STRAINS OF BACTERIA FROM THE RUMEN OF CATTLE. J Gen Microbiol. 1963 Sep;32:441–448. doi: 10.1099/00221287-32-3-441. [DOI] [PubMed] [Google Scholar]
  12. Hungate R. E. Studies on Cellulose Fermentation: III. The Culture and Isolation for Cellulose-decomposing Bacteria from the Rumen of Cattle. J Bacteriol. 1947 May;53(5):631–645. doi: 10.1128/jb.53.5.631-645.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Knox K. W., Vesk M., Work E. Relation between excreted lipopolysaccharide complexes and surface structures of a lysine-limited culture of Escherichia coli. J Bacteriol. 1966 Oct;92(4):1206–1217. doi: 10.1128/jb.92.4.1206-1217.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Latham M. J., Brooker B. E., Pettipher G. L., Harris P. J. Ruminococcus flavefaciens Cell Coat and Adhesion to Cotton Cellulose and to Cell Walls in Leaves of Perennial Ryegrass (Lolium perenne). Appl Environ Microbiol. 1978 Jan;35(1):156–165. doi: 10.1128/aem.35.1.156-165.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lev M. Vitamin K deficiency in Fusiformis nigrescens. I. Influence on whole cells and cell envelope characteristics. J Bacteriol. 1968 Jun;95(6):2317–2324. doi: 10.1128/jb.95.6.2317-2324.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Luft J. H. Ruthenium red and violet. I. Chemistry, purification, methods of use for electron microscopy and mechanism of action. Anat Rec. 1971 Nov;171(3):347–368. doi: 10.1002/ar.1091710302. [DOI] [PubMed] [Google Scholar]
  17. Pate J. L., Ordal E. J. The fine structure of Chondrococcus columnaris. I. Structure and formation of mesosomes. J Cell Biol. 1967 Oct;35(1):1–13. doi: 10.1083/jcb.35.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Patterson H., Irvin R., Costerton J. W., Cheng K. J. Ultrastructure and adhesion properties of Ruminococcus albus. J Bacteriol. 1975 Apr;122(1):278–287. doi: 10.1128/jb.122.1.278-287.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sharpe M. E., Brock J. H., Phillips B. A. Glycerol teichoic acid as an antigenic determinant in a Gram-negative bacterium Butyrivibrio fibrisolvens. J Gen Microbiol. 1975 Jun;88(2):355–363. doi: 10.1099/00221287-88-2-355. [DOI] [PMC free article] [PubMed] [Google Scholar]

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