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. 1979 Mar;37(3):414–420. doi: 10.1128/aem.37.3.414-420.1979

Emulsifier of Arthrobacter RAG-1: Chemical and Physical Properties

A Zuckerberg 1, A Diver 1, Z Peeri 1, D L Gutnick 1, E Rosenberg 1
PMCID: PMC243231  PMID: 453822

Abstract

The extracellular emulsifier of Arthrobacter RAG-1 was deproteinized by hot phenol treatment and purified by fractional precipitation with (NH4)2SO4. The active fraction, precipitating between 30 and 35% saturation [EF-RAG(UET) WA], appeared to be homogeneous by immunodiffusion and sedimentation analysis. EF-RAG(UET) WA had an intrinsic viscosity of 750 cm3/g, a sedimentation constant of 6.06S, a diffusion constant of 5.25 × 10−8 cm2 s−1, and a partial molar volume of 0.712 cm3 g−1. From these data a weight average molecular weight of 9.76 × 105 and a viscosity average molecular weight of 9.88 × 105 were calculated. EF-RAG(UET)WA contained 46.7% C, 7.01% H, and 6.06% N. Titration of the nonreducing polymer gave a single inflection point (pK′ = 3.05), corresponding to 1.5 μmol of carboxyl groups per mg. Direct estimation of O-ester and hexose content of the highly acidic polymer yielded 0.65 and 0.29 μmol/mg, respectively. Mild alkaline hydrolysis released fatty acids with an average molecular weight of about 231. Strong acid hydrolysis of EF-RAG(UET)WA yielded d-glucose (minor), d-galactosamine (major), and an unidentified amino uronic acid (major).

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Selected References

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  1. DISCHE Z. New color reactions for determination of sugars in polysaccharides. Methods Biochem Anal. 1955;2:313–358. doi: 10.1002/9780470110188.ch11. [DOI] [PubMed] [Google Scholar]
  2. David M. M., Daniel E. Subunit structure of earthworm erythrocruorin. J Mol Biol. 1974 Jul 25;87(1):89–101. doi: 10.1016/0022-2836(74)90561-0. [DOI] [PubMed] [Google Scholar]
  3. Gal A. E. Separation and identification of monosaccharides from biological materials by thin-layer chromatography. Anal Biochem. 1968 Sep;24(3):452–461. doi: 10.1016/0003-2697(68)90152-8. [DOI] [PubMed] [Google Scholar]
  4. Gutnick D. L., Rosenberg E. Oil tankers and pollution: a microbiological approach. Annu Rev Microbiol. 1977;31:379–396. doi: 10.1146/annurev.mi.31.100177.002115. [DOI] [PubMed] [Google Scholar]
  5. HANESSIAN S., HASKELL T. H. STRUCTURAL STUDIES ON STAPHYLOCOCCAL POLYSACCHARIDE ANTIGEN. J Biol Chem. 1964 Sep;239:2758–2764. [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Liau D. F., Hash J. H. Structural analysis of the surface polysaccharide of Staphylococcus aureus M. J Bacteriol. 1977 Jul;131(1):194–200. doi: 10.1128/jb.131.1.194-200.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. PERKINS H. R. A polymer containing glucose and aminohexuronic acid isolated from the cell walls of micrococcus lysodeikticus. Biochem J. 1963 Mar;86:475–483. doi: 10.1042/bj0860475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Reisfeld A., Rosenberg E., Gutnick D. Microbial degradation of crude oil: factors affecting the dispersion in sea water by mixed and pure cultures. Appl Microbiol. 1972 Sep;24(3):363–368. doi: 10.1128/am.24.3.363-368.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Reistad R. 2-Amino-2-deoxyguluronic acid: a constituent of the cell wall of Halococcus sp., strain 24. Carbohydr Res. 1974 Sep;36(2):420–423. doi: 10.1016/s0008-6215(00)83066-9. [DOI] [PubMed] [Google Scholar]
  11. Rosenberg E., Perry A., Gibson D. T., Gutnick D. L. Emulsifier of Arthrobacter RAG-1: specificity of hydrocarbon substrate. Appl Environ Microbiol. 1979 Mar;37(3):409–413. doi: 10.1128/aem.37.3.409-413.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rosenberg E., Zuckerberg A., Rubinovitz C., Gutnick D. L. Emulsifier of Arthrobacter RAG-1: isolation and emulsifying properties. Appl Environ Microbiol. 1979 Mar;37(3):402–408. doi: 10.1128/aem.37.3.402-408.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. STERN I., SHAPIRO B. A rapid and simple method for the determination of esterified fatty acids and for total fatty acids in blood. J Clin Pathol. 1953 May;6(2):158–160. doi: 10.1136/jcp.6.2.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Torii M., Sakakibara K., Kuroda K. Occurrence of 2-amino-2-deoxy-hexuronic acids as constituents of Vibrio parahaemolyticus K15 antigen. Eur J Biochem. 1973 Sep 3;37(3):401–405. doi: 10.1111/j.1432-1033.1973.tb02999.x. [DOI] [PubMed] [Google Scholar]
  15. WILLIAMSON A. R., ZAMENHOF S. The type-specific substance of Hemophilus influenzae, type d: the natural occurrence of glucosamine uronic acid. J Biol Chem. 1963 Jul;238:2255–2258. [PubMed] [Google Scholar]
  16. Wilkinson S. G., Welbourn A. P. 2-Amino-2-deoxygalacturonic acid in lipopolysaccharides from Pseudomonas aerugimosa. Biochem J. 1975 Sep;149(3):783–784. doi: 10.1042/bj1490783. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Zajjc J. E., Panchal C. J. Bio-emulsifiers. CRC Crit Rev Microbiol. 1976 Nov;5(1):39–66. doi: 10.3109/10408417609102309. [DOI] [PubMed] [Google Scholar]

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