Abstract
Two marine, Mn(II)-oxidizing bacterial cultures, BIII 45 and BIII 82, were examined spectrophotometrically at ambient temperature for their cytochrome complements. Membrane preparations from an ethylenediaminetetracetate-lysozyme treatment of 48-h cultures of both strains contained type b, c, and o cytochromes. No evidence for a type a cytochrome was noted. “Periplasmic” fractions of both strains also contained small amounts of cytochrome, including cytochrome o, but “intracellular” fractions did not. Type c cytochrome in membrane preparations of culture BIII 45 was consistently reduced by Mn(II) when the membranes were suspended in the periplasmic fraction of the culture. In the case of culture BIII 82, type c cytochrome in membrane preparations was consistently reduced by Mn(II) when the membranes were suspended in either periplasmic or intracellular fractions of the strain. Although, based on previous inhibitor studies, type b cytochrome was also expected to be reduced by Mn(II), no spectrophotometric evidence for its reduction was found, probably because not enough of it was reduced under the steady-state conditions of the experiments.
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