Abstract
The nutritional requirements of Xanthomonas campestris NRRL B1459 for optimal xanthan production were studied in a chemically defined medium. Of the carbon sources tested, a 4% sucrose or glucose medium yielded the highest xanthan titers. The further addition of certain organic acids, such as succinate, pyruvate, and α-ketoglutarate, stimulated xanthan production; excess concentrations of these organic acids inhibited xanthan formation. Certain amino acids (e.g., glutamate) and nitrate salts were superior to ammonium salts for xanthan production. Concentrations of these nitrogen sources higher than the optimal levels inhibited xanthan production while stimulating growth. Xanthan production was also sensitive to high concentrations of inorganic phosphate. High xanthan potencies, up to 30 g/kg of broth, were achieved in these shake-flask studies, in which completely defined media were used.
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- CORPE W. A. FACTORS INFLUENCING GROWTH AND POLYSACCHARIDE FORMATION BY STRAINS OF CHROMOBACTERIUM VIOLACEUM. J Bacteriol. 1964 Nov;88:1433–1441. doi: 10.1128/jb.88.5.1433-1441.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Catley B. J. Role of pH and nitrogen limitation in the elaboration of the extracellular polysaccharide pullulan by Pullularia pullulans. Appl Microbiol. 1971 Oct;22(4):650–654. doi: 10.1128/am.22.4.650-654.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DUGUID J. P., WILKINSON J. F. The influence of cultural conditions on polysaccharide production by Aerobacter aerogenes. J Gen Microbiol. 1953 Oct;9(2):174–189. doi: 10.1099/00221287-9-2-174. [DOI] [PubMed] [Google Scholar]
- Goto S., Murakawa T., Kuwahara S. Slime production by Pseudomonas aeruginosa. II. A new synthetic medium and cultural conditions suitable for slime production by Pseudomonas aeruginosa. Jpn J Microbiol. 1973 Jan;17(1):45–51. doi: 10.1111/j.1348-0421.1973.tb00702.x. [DOI] [PubMed] [Google Scholar]
- LILLY V. G., WILSON H. A., LEACH J. G. Bacterial polysaccharides. II. Laboratory-scale production of polysaccharides by species of Xanthomonas. Appl Microbiol. 1958 Mar;6(2):105–108. doi: 10.1128/am.6.2.105-108.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Williams A. G., Wimpenny J. W. Exopolysaccharide production by Pseudomonas NCIB11264 grown in batch culture. J Gen Microbiol. 1977 Sep;102(1):13–21. doi: 10.1099/00221287-102-1-13. [DOI] [PubMed] [Google Scholar]