Abstract
Ten strains of strictly anaerobic spiral organisms were isolated in pure culture from a 10(-7) dilution of bovine rumen contents. Three strains were studied in detail. These strains morphologically resembled previously isolated and described rumen treponemes except the new isolates were larger, 0.7 micrometer wide and 12 to 25 micrometer long. They rapidly fermented pectin and, less readily, L-arabinose, inulin, and sucrose. Acetic and formic acid were the main fermentation products from pectin; small amounts of succinic acid were also formed.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BITTER T., MUIR H. M. A modified uronic acid carbazole reaction. Anal Biochem. 1962 Oct;4:330–334. doi: 10.1016/0003-2697(62)90095-7. [DOI] [PubMed] [Google Scholar]
- BRYANT M. P. The isolation and characteristics of a spirochete from the bovine rumen. J Bacteriol. 1952 Sep;64(3):325–335. doi: 10.1128/jb.64.3.325-335.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HUNGATE R. E. The anaerobic mesophilic cellulolytic bacteria. Bacteriol Rev. 1950 Mar;14(1):1–49. doi: 10.1128/br.14.1.1-49.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tomerska H. Decomposition of pectin in vitro by pure strains of rumen bacteria. Acta Microbiol Pol B. 1971;3(2):107–115. [PubMed] [Google Scholar]
- Wojciechowicz M., Ziołecki A. Pectinolytic enzymes of large rumen treponemes. Appl Environ Microbiol. 1979 Jan;37(1):136–142. doi: 10.1128/aem.37.1.136-142.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ziolecki A., Kwiatkowska E. Gas chromatography of C1 to C5 fatty acids in rumen fluid and fermentation media. J Chromatogr. 1973 Jun;80(2):250–254. doi: 10.1016/s0021-9673(01)85338-3. [DOI] [PubMed] [Google Scholar]
- Ziolecki A., Tomerska H., Wojciechowicz M. Characteristics of pure cultures of Borrelia sp. isolated from the bovine rumen. Acta Microbiol Pol B. 1975;7(1):45–50. [PubMed] [Google Scholar]