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. 1979 Jul;38(1):13–17. doi: 10.1128/aem.38.1.13-17.1979

Examination of Methods for Enumerating Hemicellulose-Utilizing Bacteria in the Rumen

Penelope A Henning 1
PMCID: PMC243427  PMID: 16345407

Abstract

Counts of colonies that developed after 4 days on agar medium containing 0.3% xylan and preincubated rumen fluid were similar to counts of xylanolytic bacteria obtained when total culturable counts were multiplied by the percentage of isolates capable of producing acid from xylan. Shortening the incubation period reduced the chance of including satellite colonies of non-xylanolytic organisms in the count. Nearly all of the xylanolytic isolates irrespective of the medium from which they were isolated degraded and utilized xylan extensively. The use of a culture medium containing a high concentration (3%) of xylan is also described. The number of colonies capable of producing clearings in this medium was less than 10% of the total culturable counts. Isolates from such colonies were shown to produce diffusible (extracellular) xylanases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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