Abstract
A procedure is presented for screening the quality of feed in respect to ochratoxin A contamination based upon the analysis of ochratoxin A in pig blood. Representative samples from large feed lots may be obtained by using pigs as in vivo sample collectors which enrich the toxin and forms homogeneous samples in the blood. The spectrofluorometric procedure for ochratoxin A analysis (K. Hult and S. Gatenbeck, J. Assoc. Off. Anal. Chem. 59:128-129, 1976) has been adapted to pig blood and has been simplified to involve only three extraction steps. A volume of 2.5 ml of blood or plasma is needed, and the detection limit is 2 ng of ochratoxin A per ml. The disappearance of ochratoxin A from pig blood as a function of time has been studied. A feeding experiment with ochratoxin A has been performed, and the time course of the concentration of ochratoxin A in blood has been followed during the experiment.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Hult K., Gatenbeck S. A spectrophotometric procedure, using carboxypeptidase A, for the quantitative measurement of ochratoxin A. J Assoc Off Anal Chem. 1976 Jan;59(1):128–129. [PubMed] [Google Scholar]
- Krogh P., Elling F., Hald B., Larsen A. E., Lillehoj E. B., Madsen A., Mortensen H. P. Time-dependent disappearance of ochratoxin A residues in tissues of bacon pigs. Toxicology. 1976 Aug-Sep;6(2):235–242. doi: 10.1016/0300-483x(76)90025-1. [DOI] [PubMed] [Google Scholar]
- Krogh P. Ochratoxin A residues in tissues of slaughter pigs with nephropathy. Nord Vet Med. 1977 Sep;29(9):402–405. [PubMed] [Google Scholar]
- Rutqvist L., Björklund N. E., Hult K., Hökby E., Carlsson B. Ochratoxin A as the cause of spontaneous nephropathy in fattening pigs. Appl Environ Microbiol. 1978 Dec;36(6):920–925. doi: 10.1128/aem.36.6.920-925.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]