Abstract
An Aspergillus niger mutant strain, VTT-D-80144, with an improvement of three- to fourfold in the production of extracellular beta-galactosidase was isolated after mutagenesis. The production of beta-galactosidase by this mutant was unaffected by fermentor size, and the enzyme was also suitable for immobilization.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dickson R. C., Markin J. S. Molecular cloning and expression in E. coli of a yeast gene coding for beta-galactosidase. Cell. 1978 Sep;15(1):123–130. doi: 10.1016/0092-8674(78)90088-0. [DOI] [PubMed] [Google Scholar]
- Fantes P. A., Roberts C. F. Beta-galactosidase activity and lactose utilization in Aspergillus nidulans. J Gen Microbiol. 1973 Aug;77(2):417–486. doi: 10.1099/00221287-77-2-417. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Nevalainen K. M., Palva E. T. Production of extracellular enzymes in mutants isolated from trichoderma viride unable to hydrolyze cellulose. Appl Environ Microbiol. 1978 Jan;35(1):11–16. doi: 10.1128/aem.35.1.11-16.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ratzkin B., Carbon J. Functional expression of cloned yeast DNA in Escherichia coli. Proc Natl Acad Sci U S A. 1977 Feb;74(2):487–491. doi: 10.1073/pnas.74.2.487. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SMOGYI M. Notes on sugar determination. J Biol Chem. 1952 Mar;195(1):19–23. [PubMed] [Google Scholar]
- Vapnek D., Hautala J. A., Jacobson J. W., Giles N. H., Kushner S. R. Expression in Escherichia coli K-12 of the structural gene for catabolic dehydroquinase of Neurospora crassa. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3508–3512. doi: 10.1073/pnas.74.8.3508. [DOI] [PMC free article] [PubMed] [Google Scholar]