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. 1981 Apr;41(4):1043–1045. doi: 10.1128/aem.41.4.1043-1045.1981

Detection of Rotavirus in Sewage

Jochen Steinmann 1
PMCID: PMC243854  PMID: 16345746

Abstract

For detection of rotavirus, domestic sewage was concentrated by two different methods: (i) adsorption to and elution from positively charged Seitz filters, followed by ultracentrifugation, and (ii) chemical precipitation. The concentrated fluids were tested by an enzyme-linked immunosorbent assay and electron microscopy. In 6 of 24 (25%) samples, rotavirus was detectable after the combined filtration and ultracentrifugation technique with both an enzyme-linked immunosorbent assay and electron microscopy. No positive results were obtained after chemical precipitation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carlson J. A., Middleton P. J., Szymanski M. T., Huber J., Petric M. Fatal rotavirus gastroenteritis: an analysis of 21 cases. Am J Dis Child. 1978 May;132(5):477–479. doi: 10.1001/archpedi.1978.02120300037006. [DOI] [PubMed] [Google Scholar]
  2. Estes M. K., Graham D. Y., Smith E. M., Gerba C. P. Rotavirus stability and inactivation. J Gen Virol. 1979 May;43(2):403–409. doi: 10.1099/0022-1317-43-2-403. [DOI] [PubMed] [Google Scholar]
  3. Farrah S. R., Goyal S. M., Gerba C. P., Conklin R. H., Smith E. M. Comparison between adsorption of poliovirus and rotavirus by aluminum hydroxide and activated sludge flocs. Appl Environ Microbiol. 1978 Feb;35(2):360–363. doi: 10.1128/aem.35.2.360-363.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hurst C. J., Gerba C. P. Stability of simian rotavirus in fresh and estuarine water. Appl Environ Microbiol. 1980 Jan;39(1):1–5. doi: 10.1128/aem.39.1.1-5.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kalica A. R., Purcell R. H., Sereno M. M., Wyatt R. G., Kim H. W., Chanock R. M., Kapikian A. Z. A microtiter solid phase radioimmunoassay for detection of the human reovirus-like agent in stools. J Immunol. 1977 Apr;118(4):1275–1279. [PubMed] [Google Scholar]
  6. Kapikian A. Z., Kim H. W., Wyatt R. G., Cline W. L., Arrobio J. O., Brandt C. D., Rodriguez W. J., Sack D. A., Chanock R. M., Parrott R. H. Human reovirus-like agent as the major pathogen associated with "winter" gastroenteritis in hospitalized infants and young children. N Engl J Med. 1976 Apr 29;294(18):965–972. doi: 10.1056/NEJM197604292941801. [DOI] [PubMed] [Google Scholar]
  7. Kelen A. E., Hathaway A. E., McLeod D. A. Rapid detection of Australia-SH antigen and antibody by a simple and sensitive technique of immunoelectronmicroscopy. Can J Microbiol. 1971 Jul;17(7):993–1000. doi: 10.1139/m71-157. [DOI] [PubMed] [Google Scholar]
  8. Lycke E., Blomberg J., Berg G., Eriksson A., Madsen L. Epidemic acute diarrhoea in adults associated with infantile gastroenteritis virus. Lancet. 1978 Nov 11;2(8098):1056–1057. doi: 10.1016/s0140-6736(78)92389-9. [DOI] [PubMed] [Google Scholar]
  9. Moosai R. B., Gardner P. S., Almeida J. D., Greenaway M. A. A simple immunofluorescent technique for the detection of human rotavirus. J Med Virol. 1979;3(3):189–194. doi: 10.1002/jmv.1890030304. [DOI] [PubMed] [Google Scholar]
  10. Morens D. M., Zweighaft R. M., Vernon T. M., Gary G. W., Eslien J. J., Wood B. T., Holman R. C., Dolin R. A waterborne outbreak of gastroenteritis with secondary person-to-person spread. Association with a viral agent. Lancet. 1979 May 5;1(8123):964–966. doi: 10.1016/s0140-6736(79)91734-3. [DOI] [PubMed] [Google Scholar]
  11. Ramia S., Sattar S. A. Concentration of seeded simian rotavirus SA-11 from potable waters by using talc-celite layers and hydroextraction. Appl Environ Microbiol. 1980 Mar;39(3):493–499. doi: 10.1128/aem.39.3.493-499.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wyatt R. G., Gill V. W., Sereno M. M., Kalica A. R., VanKirk D. H., Chanock R. M., Kapikian A. Z. Letter: Probable in-vitro cultivation of human reovirus-like agent of infantile diarroea. Lancet. 1976 Jan 10;1(7950):98–99. doi: 10.1016/s0140-6736(76)90202-6. [DOI] [PubMed] [Google Scholar]
  13. Yolken R. H., Kim H. W., Clem T., Wyatt R. G., Kalica A. R., Chanock R. M., Kapikian A. Z. Enzyme-linked immunosorbent assay (ELISA) for detection of human reovirus-like agent of infantile gastroenteritis. Lancet. 1977 Aug 6;2(8032):263–267. doi: 10.1016/s0140-6736(77)90951-5. [DOI] [PubMed] [Google Scholar]
  14. Zissis G., Lambert J. P., De Kegel D. Routine diagnosis of human rotaviruses in stools. J Clin Pathol. 1978 Feb;31(2):175–178. doi: 10.1136/jcp.31.2.175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. von Bonsdorff C. H., Hovi T., Mäkelä P., Mörttinen A. Rotavirus infections in adults in association with acute gastroenteritis. J Med Virol. 1978;2(1):21–28. doi: 10.1002/jmv.1890020105. [DOI] [PubMed] [Google Scholar]

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