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. 1981 Apr;41(4):1043–1045. doi: 10.1128/aem.41.4.1043-1045.1981

Detection of Rotavirus in Sewage

Jochen Steinmann 1
PMCID: PMC243854  PMID: 16345746

Abstract

For detection of rotavirus, domestic sewage was concentrated by two different methods: (i) adsorption to and elution from positively charged Seitz filters, followed by ultracentrifugation, and (ii) chemical precipitation. The concentrated fluids were tested by an enzyme-linked immunosorbent assay and electron microscopy. In 6 of 24 (25%) samples, rotavirus was detectable after the combined filtration and ultracentrifugation technique with both an enzyme-linked immunosorbent assay and electron microscopy. No positive results were obtained after chemical precipitation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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