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. 1981 May;41(5):1132–1138. doi: 10.1128/aem.41.5.1132-1138.1981

Comparison of Two Direct-Count Methods for Determining Metabolizing Bacteria in Freshwater †

James S Maki 1, Charles C Remsen 1
PMCID: PMC243879  PMID: 16345767

Abstract

Planktonic bacteria collected from several freshwater environments and cultured bacteria were used to compare two methods for determining the numbers of metabolizing bacteria. The methods used were (i) reduction of 2-(ρ-iodophenyl)-3-(ρ-nitrophenyl)-5-phenyl tetrazolium chloride 2-(ρ-iodophenyl)-3-(ρ-nitrophenyl)-5-phenyl to tetrazolium chloride-formazan and (ii) elongation of cells by using yeast extract and nalidixic acid. No statistically significant difference was found between methods in determining metabolizing bacteria, although significant differences (P < 0.05) were found when comparing numbers of total bacteria. A combination of the two methods yielded significant changes, both positive and negative, in the numbers of metabolizing bacteria.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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