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. 1981 Jul;42(1):56–60. doi: 10.1128/aem.42.1.56-60.1981

Serogrouping of motile Aeromonas species isolated from healthy and moribund fish.

D Leblanc, K R Mittal, G Olivier, R Lallier
PMCID: PMC243960  PMID: 7259164

Abstract

A total of 195 strains of motile Aeromonas isolated from fish were characterized as Aeromonas hydrophila and Aeromonas sobria. In view of the frequency of isolation and the importance of motile Aeromonas species as fish pathogens, a serological classificaton of these organisms was attempted. Antisera were prepared in rabbits against formalinized whole cell suspensions and against boiled cells of 12 different isolates. Seventy-six strains could be grouped by tube agglutination with whole cells as antigen and anti-whole cell antiserum. However, only 39 strains were typable with anti-O serum. Differentiation was made between heat-stable antigens and heat-labile antigens which did not block the O agglutination reaction. The same heat-labile antigen could be associated with different heat-stable particulate antigens, and a relationship was observed between the heat-stable particulate antigens and the virulence of A. hydrophila for fish. In addition to these two types of antigen, motile Aeromonas possessed heat-stable soluble antigens which could be detected by indirect hemagglutination. One strain seemed to possess various heat-stable soluble antigens; so far, however, it does not appear to be feasible to use these antigens for serology. Finally, we also observed cross-reactions between some A. hydrophila and A. sobria strains.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boulanger Y., Lallier R., Cousineau G. Isolation of enterotoxigenic Aeromonas from fish. Can J Microbiol. 1977 Sep;23(9):1161–1164. doi: 10.1139/m77-174. [DOI] [PubMed] [Google Scholar]
  2. Davis W. A., 2nd, Kane J. G., Garagusi V. F. Human aeromonas infections: a review of the literature and a case report of endocarditis. Medicine (Baltimore) 1978 May;57(3):267–277. [PubMed] [Google Scholar]
  3. Fliermans C. B., Gorden R. W., Hazen T. C., Esch G. W. Aeromonas distribution and survival in a thermally altered lake. Appl Environ Microbiol. 1977 Jan;33(1):114–122. doi: 10.1128/aem.33.1.114-122.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fliermans C. B., Hazen T. C. Immunofluorescence of Aeromonas hydrophila as measured by fluorescence photometric microscopy. Can J Microbiol. 1980 Feb;26(2):161–168. doi: 10.1139/m80-024. [DOI] [PubMed] [Google Scholar]
  5. MacInnes J. I., Trust T. J., Crosa J. H. Deoxyribonucleic acid relationships among members of the genus Aeromonas. Can J Microbiol. 1979 May;25(5):579–586. doi: 10.1139/m79-083. [DOI] [PubMed] [Google Scholar]
  6. Mittal K. R., Ingram D. G. Factors involved in bactericidal activity of sheep serum. Am J Vet Res. 1975 Aug;36(08):1183–1187. [PubMed] [Google Scholar]
  7. Mittal K. R., Lalonde G., Leblanc D., Olivier G., Lallier R. Aeromonas hydrophila in rainbow trout: relation between virulence and surface characteristics. Can J Microbiol. 1980 Dec;26(12):1501–1503. doi: 10.1139/m80-248. [DOI] [PubMed] [Google Scholar]
  8. Paterson W. D., Douey D., Desautels D. Relationships between selected strains of typical and atypical Aeromonas salmonicida, Aeromonas hydrophila, and Haemophilus piscium. Can J Microbiol. 1980 May;26(5):588–598. doi: 10.1139/m80-104. [DOI] [PubMed] [Google Scholar]
  9. Popoff M., Véron M. A taxonomic study of the Aeromonas hydrophila-Aeromonas punctata group. J Gen Microbiol. 1976 May;94(1):11–22. doi: 10.1099/00221287-94-1-11. [DOI] [PubMed] [Google Scholar]
  10. Shaw D. H., Hodder H. J. Lipopolysaccharides of the motile aeromonads; core oligosaccharide analysis as an aid to taxonomic classification. Can J Microbiol. 1978 Jul;24(7):864–868. doi: 10.1139/m78-143. [DOI] [PubMed] [Google Scholar]
  11. Trust T. J., Sparrow R. A. The bacterial flora in the alimentary tract of freshwater salmonid fishes. Can J Microbiol. 1974 Sep;20(9):1219–1228. doi: 10.1139/m74-188. [DOI] [PubMed] [Google Scholar]
  12. de Meuron P. A., Peduzzi R. Caractérisation de souches du genre Aeromonas isolées chez des poissons d'eau douce et quelques reptiles. Zentralbl Veterinarmed B. 1979 Mar;26(2):153–167. [PubMed] [Google Scholar]

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