Abstract
Reference strains and clinical isolates of Peptococcus and Peptostreptococcus spp. were evaluated for their growth response in supplemented thioglycolate-yeast extract media. Supplements used included various combinations of hemin, menadione, sodium bicarbonate, and Tween 80. Parallel studies were done to compare the efficiency of recovery of viable cells grown in thioglycolate-based media and Wilkins-Chalgren broth and agar. In addition, the effects of age of the medium and medium storage on viable cell yields for reference strains were determined. Reference strains grown in freshly prepared thioglycolate-yeast extract medium supplemented with sodium bicarbonate produced a 10-fold greater increase in the number of viable cells recovered after 24 h of incubation than did the same organisms cultivated in Wilkins-Chalgren medium. The efficiency of recovery of organisms when either mid-logarithmic- or mid-stationary-phase cells were used to prepare standardized inocula was similar. The results suggest that thioglycolate-yeast extract medium supplemented with sodium bicarbonate is more productive than Wilkins-Chalgren medium for the cultivation of anaerobic gram-positive cocci and may represent a suitable alternative for antimicrobial susceptibility testing of these organisms.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bartlett J. G., Sullivan-Sigler N., Louie T. J., Gorbach S. L. Anaerobes survive in clinical specimens despite delayed processing. J Clin Microbiol. 1976 Feb;3(2):133–136. doi: 10.1128/jcm.3.2.133-136.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Frölander F., Carlsson J. Bactericidal effect of anaerobic broth exposed to atmospheric oxygen tested on Peptostreptococcus anaerobius. J Clin Microbiol. 1977 Aug;6(2):117–123. doi: 10.1128/jcm.6.2.117-123.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GIBBONS R. J., MACDONALD J. B. Hemin and vitamin K compounds as required factors for the cultivation of certain strains of Bacteroides melaninogenicus. J Bacteriol. 1960 Aug;80:164–170. doi: 10.1128/jb.80.2.164-170.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hanson C. W., Martin W. J. Evaluation of enrichment, storage, and age of blood agar medium in relation to its ability to support growth of anaerobic bacteria. J Clin Microbiol. 1976 Nov;4(5):394–399. doi: 10.1128/jcm.4.5.394-399.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Loesche W. J. Oxygen sensitivity of various anaerobic bacteria. Appl Microbiol. 1969 Nov;18(5):723–727. doi: 10.1128/am.18.5.723-727.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- QUINTO G., SEBALD II. IDENTIFICATION OF THREE HEMIN-REQUIRING BACTEROIDES STRAINS. Am J Med Technol. 1964 Nov-Dec;30:381–384. [PubMed] [Google Scholar]
- Rosenblatt J. E., Fallon A., Finegold S. M. Comparison of methods for isolation of anaerobic bacteria from clinical specimens. Appl Microbiol. 1973 Jan;25(1):77–85. doi: 10.1128/am.25.1.77-85.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stalons D. R., Thornsberry C., Dowell V. R., Jr Effect of culture medium and carbon dioxide concentration on growth of anaerobic bacteria commonly encountered in clinical specimens. Appl Microbiol. 1974 Jun;27(6):1098–1104. doi: 10.1128/am.27.6.1098-1104.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tally F. P., Stewart P. R., Sutter V. L., Rosenblatt J. E. Oxygen tolerance of fresh clinical anaerobic bacteria. J Clin Microbiol. 1975 Feb;1(2):161–164. doi: 10.1128/jcm.1.2.161-164.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wilkins T. D., Chalgren S. L., Jimenez-Ulate F., Drake C. R., Jr, Johnson J. L. Inhibition of Bacteroides fragilis on blood agar plates and reversal of inhibition by added hemin. J Clin Microbiol. 1976 Mar;3(3):359–363. doi: 10.1128/jcm.3.3.359-363.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]