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. 1982 May;43(5):1116–1124. doi: 10.1128/aem.43.5.1116-1124.1982

Microbial Activities in Undecompressed and Decompressed Deep-Seawater Samples †

Holger W Jannasch 1, Carl O Wirsen 1
PMCID: PMC244195  PMID: 16346008

Abstract

Microbial transformations of 14C-labeled substrates (sodium glutamate, Casamino Acids, glucose, and sodium acetate) were measured in undecompressed seawater samples collected from depths of 1,800 to 6,000 m, during 14- to 21-day incubation periods at in situ temperature (3°C). Each substrate was tested at two concentrations (ca. 0.5 and 5.0 μg/ml) and two in situ pressures. The data were compared to 1-atmosphere (ca. 1.013 × 102 kPa) controls. The rates of 14C incorporation and 14CO2 production as well as the amounts of total substrate utilization were generally lower at pressure than in the decompressed controls but were significantly different for each of the four substrates used. The utilization of acetate was the least affected by pressure; rates were similar to those measured at 1 atmosphere in two out of four experiments. In contrast, transformation rates of the amino acids at pressure averaged to only 38% of those in the controls. A single but reproducible “barophilic” response was observed with glucose as a substrate in samples collected from a depth of 4,500 m at a specific area in the northwestern Atlantic Ocean. Except for this latter set of experiments, the transformation of all substrates showed an increased lag period at pressure as compared to the 1-atmosphere controls.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dietz A. S., Yayanos A. A. Silica gel media for isolating and studying bacteria under hydrostatic pressure. Appl Environ Microbiol. 1978 Dec;36(6):966–968. doi: 10.1128/aem.36.6.966-968.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. HERBERT D., ELSWORTH R., TELLING R. C. The continuous culture of bacteria; a theoretical and experimental study. J Gen Microbiol. 1956 Jul;14(3):601–622. doi: 10.1099/00221287-14-3-601. [DOI] [PubMed] [Google Scholar]
  3. Jannasch H. J., Wirsen C. O., Taylor C. D. Undecompressed microbial populations from the deep sea. Appl Environ Microbiol. 1976 Sep;32(3):360–367. doi: 10.1128/aem.32.3.360-367.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Jannasch H. W., Eimhjellen K., Wirsen C. O., Farmanfarmaian A. Microbial degradation of organic matter in the deep sea. Science. 1971 Feb 19;171(3972):672–675. doi: 10.1126/science.171.3972.672. [DOI] [PubMed] [Google Scholar]
  5. Jannasch H. W., Wirsen C. O. Deep-Sea Microorganisms: In situ Response to Nutrient Enrichment. Science. 1973 May 11;180(4086):641–643. doi: 10.1126/science.180.4086.641. [DOI] [PubMed] [Google Scholar]
  6. Jannasch H. W., Wirsen C. O. Retrieval of concentrated and undecompressed microbial populations from the deep sea. Appl Environ Microbiol. 1977 Mar;33(3):642–646. doi: 10.1128/aem.33.3.642-646.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kuznetsov S. I., Dubinina G. A., Lapteva N. A. Biology of oligotrophic bacteria. Annu Rev Microbiol. 1979;33:377–387. doi: 10.1146/annurev.mi.33.100179.002113. [DOI] [PubMed] [Google Scholar]
  8. Marquis R. E. High-pressure microbial physiology. Adv Microb Physiol. 1976;14(11):159–241. doi: 10.1016/s0065-2911(08)60228-3. [DOI] [PubMed] [Google Scholar]
  9. Morita R. Y. Psychrophilic bacteria. Bacteriol Rev. 1975 Jun;39(2):144–167. doi: 10.1128/br.39.2.144-167.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Yayanos A. A., Dietz A. S., VAN Boxtel R. Isolation of a deep-sea barophilic bacterium and some of its growth characteristics. Science. 1979 Aug 24;205(4408):808–810. doi: 10.1126/science.205.4408.808. [DOI] [PubMed] [Google Scholar]
  11. ZOBELL C. E., MORITA R. Y. Barophilic bacteria in some deep sea sediments. J Bacteriol. 1957 Apr;73(4):563–568. doi: 10.1128/jb.73.4.563-568.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]

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