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Comparative and Functional Genomics logoLink to Comparative and Functional Genomics
. 2002 Feb;3(1):57–66. doi: 10.1002/cfg.130

High-Throughput SNP Genotyping

Suzanne Jenkins 1,✉, Neil Gibson 1
PMCID: PMC2447245  PMID: 18628885

Abstract

Whole genome approaches using single nucleotide polymorphism (SNP) markers have the potential to transform complex disease genetics and expedite pharmacogenetics research. This has led to a requirement for high-throughput SNP genotyping platforms. Development of a successful high-throughput genotyping platform depends on coupling reliable assay chemistry with an appropriate detection system to maximise efficiency with respect to accuracy, speed and cost. Current technology platforms are able to deliver throughputs in excess of 100 000 genotypes per day, with an accuracy of >99%, at a cost of 20–30 cents per genotype. In order to meet the demands of the coming years, however, genotyping platforms need to deliver throughputs in the order of one million genotypes per day at a cost of only a few cents per genotype. In addition, DNA template requirements must be minimised such that hundreds of thousands of SNPs can be interrogated using a relatively small amount of genomic DNA. As such, it is predicted that the next generation of high-throughput genotyping platforms will exploit large-scale multiplex reactions and solid phase assay detection systems.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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