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. 1974 Jul;119(1):144–151. doi: 10.1128/jb.119.1.144-151.1974

R62, a Naturally Occurring Hybrid R Plasmid

Patricia Guerry 1, Stanley Falkow 1, Naomi Datta 1
PMCID: PMC245584  PMID: 4276057

Abstract

R62, a naturally occurring R factor, was shown to be a single deoxyribonucleic acid molecule composed of polynucleotide sequences typical of I group plasmids and also sequences typical of the N group. It determined I pili and belonged to the Iα compatibility group. Although compatible with plasmids of group N, R62 showed complex genetic reactions with N plasmids which are described and interpreted. It is concluded that R62 was the product of illegitimate recombination between an I group and an N group plasmid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bazaral M., Helinski D. R. Characterization of multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 Oct;7(10):3513–3520. doi: 10.1021/bi00850a028. [DOI] [PubMed] [Google Scholar]
  2. Clewell D. B., Helinski D. R. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159–1166. doi: 10.1073/pnas.62.4.1159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clowes R. C. Molecular structure of bacterial plasmids. Bacteriol Rev. 1972 Sep;36(3):361–405. doi: 10.1128/br.36.3.361-405.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coetzee J. N., Datta N., Hedges R. W. R factors from Proteus rettgeri. J Gen Microbiol. 1972 Oct;72(3):543–552. doi: 10.1099/00221287-72-3-543. [DOI] [PubMed] [Google Scholar]
  5. Cohen S. N., Miller C. A. Non-chromosomal antibiotic resistance in bacteria. II. Molecular nature of R-factors isolated from Proteus mirabilis and Escherichia coli. J Mol Biol. 1970 Jun 28;50(3):671–687. doi: 10.1016/0022-2836(70)90092-6. [DOI] [PubMed] [Google Scholar]
  6. Datta N., Hedges R. W. An I pilus-determining R factor with anomalous compatibility properties, mobilizing a gentamicin-resistance plasmid. J Gen Microbiol. 1973 Jul;77(1):11–17. doi: 10.1099/00221287-77-1-11. [DOI] [PubMed] [Google Scholar]
  7. Datta N., Hedges R. W. Compatibility groups among fi - R factors. Nature. 1971 Nov 26;234(5326):222–223. doi: 10.1038/234222a0. [DOI] [PubMed] [Google Scholar]
  8. Datta N., Hedges R. W., Shaw E. J., Sykes R. B., Richmond M. H. Properties of an R factor from Pseudomonas aeruginosa. J Bacteriol. 1971 Dec;108(3):1244–1249. doi: 10.1128/jb.108.3.1244-1249.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Datta N., Kontomichalou P. Penicillinase synthesis controlled by infectious R factors in Enterobacteriaceae. Nature. 1965 Oct 16;208(5007):239–241. doi: 10.1038/208239a0. [DOI] [PubMed] [Google Scholar]
  10. Davies J., Brzezinska M., Benveniste R. The problems of drug-resistant pathogenic bacteria. R factors: biochemical mechanisms of resistance to aminoglycoside antibiotics. Ann N Y Acad Sci. 1971 Jun 11;182:226–233. doi: 10.1111/j.1749-6632.1971.tb30659.x. [DOI] [PubMed] [Google Scholar]
  11. Falkow S., Tompkins L. S., Silver R. P., Guerry P., Le Blanc D. J. The problems of drug-resistant pathogenic bacteria. The replication of R-factor DNA in Escherichia coli K-12 following conjugation. Ann N Y Acad Sci. 1971 Jun 11;182:153–171. doi: 10.1111/j.1749-6632.1971.tb30654.x. [DOI] [PubMed] [Google Scholar]
  12. Guerry P., Falkow S. Polynucleotide sequence relationships among some bacterial plasmids. J Bacteriol. 1971 Jul;107(1):372–374. doi: 10.1128/jb.107.1.372-374.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hedges R. W., Datta N., Coetzee J. N., Dennison S. R factors from Proteus morganii. J Gen Microbiol. 1973 Aug;77(2):249–259. doi: 10.1099/00221287-77-2-249. [DOI] [PubMed] [Google Scholar]
  14. Hedges R. W., Datta N., Kontomichalou P., Smith J. T. Molecular specificities of R factor-determined beta-lactamases: correlation with plasmid compatibility. J Bacteriol. 1974 Jan;117(1):56–62. doi: 10.1128/jb.117.1.56-62.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hedges R. W., Datta N. Plasmids determining I pili constitute a compatibility complex. J Gen Microbiol. 1973 Jul;77(1):19–25. doi: 10.1099/00221287-77-1-19. [DOI] [PubMed] [Google Scholar]
  16. Hedges R. W. Phenotypic characterization of fi- R factors determining the restriction and modification hsp II specificity. Mol Gen Genet. 1972;115(3):225–233. doi: 10.1007/BF00268886. [DOI] [PubMed] [Google Scholar]
  17. Hedges R. W. Resistance to spectinomycin determined by R factors of various compatibility groups. J Gen Microbiol. 1972 Sep;72(2):407–409. doi: 10.1099/00221287-72-2-407. [DOI] [PubMed] [Google Scholar]
  18. Jenkins P. H., Drabble W. T. -lactamases of R factors derived from Shigella and Salmonella strains. J Bacteriol. 1971 Oct;108(1):159–165. doi: 10.1128/jb.108.1.159-165.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  20. Lawn A. M., Meynell G. G., Meynell E., Datta N. Sex pili and the classification of sex factors in the enterobacteriaceae. Nature. 1967 Oct 28;216(5113):343–346. doi: 10.1038/216343a0. [DOI] [PubMed] [Google Scholar]
  21. LeBlanc D. J., Falkow S. Studies on superinfection immunity among transmissible plasmids in Escherichia coli. J Mol Biol. 1973 Mar 15;74(4):689–701. doi: 10.1016/0022-2836(73)90057-0. [DOI] [PubMed] [Google Scholar]
  22. Meynell E. Pseudo-fi + I-like sex factors, R62(I), selective for increased pilus synthesis. J Bacteriol. 1973 Jan;113(1):502–503. doi: 10.1128/jb.113.1.502-503.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Meynell G. G., Lawn A. M. Filamentous phages specific for the I sex factor. Nature. 1968 Mar 23;217(5134):1184–1186. doi: 10.1038/2171184a0. [DOI] [PubMed] [Google Scholar]
  24. Nisioka T., Mitani M., Clowes R. Composite circular forms of R factor deoxyribonucleic acid molecules. J Bacteriol. 1969 Jan;97(1):376–385. doi: 10.1128/jb.97.1.376-385.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Romero E., Meynell E. Covert fi- R factors in fi+ R+ strains of bacteria. J Bacteriol. 1969 Feb;97(2):780–786. doi: 10.1128/jb.97.2.780-786.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Silver R. P., Falkow S. Specific labeling and physical characterization of R-factor deoxyribonucleic acid in Escherichia coli. J Bacteriol. 1970 Oct;104(1):331–339. doi: 10.1128/jb.104.1.331-339.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Watanabe T., Sakaizumi S., Furuse C. Superinfection with R factors by transduction in Escherichia coli and Salmonella typhimurium. J Bacteriol. 1968 Nov;96(5):1796–1802. doi: 10.1128/jb.96.5.1796-1802.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Young E. T., 2nd, Sinsheimer R. L. Vegetative bacteriophage lambda-DNA. II. Physical characterization and replication. J Mol Biol. 1967 Nov 28;30(1):165–200. doi: 10.1016/0022-2836(67)90251-3. [DOI] [PubMed] [Google Scholar]

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