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. 1974 Oct;120(1):38–42. doi: 10.1128/jb.120.1.38-42.1974

Induction of Sporulation During Synchronized Chromosome Replication in Bacillus subtilis

J Mandelstam 1, Sonia A Higgs 1
PMCID: PMC245727  PMID: 4213734

Abstract

Synchronous chromosome replication was obtained in a culture of Bacillus subtilis temperature-sensitive mutants growing in a rich medium. At intervals during this replication, samples of cells were transferred to a poor medium to induce sporulation. The results show that the capacity of B. subtilis for induced sporulation reaches a peak about 15 min after chromosome replication has begun. This capacity then declines rapidly, but can be restored by initiating a new round of deoxyribonucleic acid replication. The possibility is discussed that sporulation can be induced only when the chromosome replication fork is passing through a stage 0 operon and that this may be located in the cysA-sulR region of the chromosome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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