Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1974 Oct;120(1):253–265. doi: 10.1128/jb.120.1.253-265.1974

Isolation and Characterization of Rifampin-Resistant and Streptolydigin-Resistant Mutants of Bacillus subtilis with Altered Sporulation Properties

Abraham L Sonenshein 1, Brigitte Cami 1, Jean Brevet 1, Richard Cote 1
PMCID: PMC245758  PMID: 4370705

Abstract

Mutants of Bacillus subtilis with altered deoxyribonucleic-dependent ribonucleic acid polymerase activity have been isolated and characterized. These mutants, selected as strains resistant to rifampin or streptolydigin, demonstrate drug-resistant in vitro ribonucleic acid synthesis. Sporeforming ability and support of phage infection are altered in many of the mutants. Mutations to rifampin and streptolydigin resistance have been located on the B. subtilis chromosome and ordered relative to the markers cysA14 and str.

Full text

PDF
256

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARONSON A. I. CHARACTERIZATION OF MESSENGER RNA IN SPORULATING BACILLUS CEREUS. J Mol Biol. 1965 Mar;11:576–588. doi: 10.1016/s0022-2836(65)80012-2. [DOI] [PubMed] [Google Scholar]
  2. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brevet J. Direct assay for sigma factor activity and demonstration of the loss of this activity during sporulation in Bacillus subtilis. Mol Gen Genet. 1974 Feb 6;128(3):223–221. doi: 10.1007/BF00267111. [DOI] [PubMed] [Google Scholar]
  4. DiCioccio R. A., Strauss N. Patterns of transcription in Bacillus subtilis during sporulation. J Mol Biol. 1973 Jun 25;77(2):325–336. doi: 10.1016/0022-2836(73)90338-0. [DOI] [PubMed] [Google Scholar]
  5. Doi R. H., Brown L. R., Rodgers G., Hsu Y. Bacillus subtilis mutant altered in spore morphology and in RNA polymerase activity. Proc Natl Acad Sci U S A. 1970 Jun;66(2):404–410. doi: 10.1073/pnas.66.2.404. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Duffy J. J., Geiduschek E. P. Transcription specificity of an RNA polymerase fraction from bacteriophage SP01-infected B. subtilis. FEBS Lett. 1973 Aug 15;34(2):172–174. doi: 10.1016/0014-5793(73)80786-0. [DOI] [PubMed] [Google Scholar]
  7. Greenleaf A. L., Linn T. G., Losick R. Isolation of a new RNA polymerase-binding protein from sporulating Bacillus subtilis. Proc Natl Acad Sci U S A. 1973 Feb;70(2):490–494. doi: 10.1073/pnas.70.2.490. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Guespin-Michel J. E. Phenotypic reversion in some early blocked sporulation mutants of Bacillus subtilis: isolation and phenotype identification of partial revertants. J Bacteriol. 1971 Oct;108(1):241–247. doi: 10.1128/jb.108.1.241-247.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Harford N., Sueoka N. Chromosomal location of antibiotic resistance markers in Bacillus subtilis. J Mol Biol. 1970 Jul 28;51(2):267–286. doi: 10.1016/0022-2836(70)90142-7. [DOI] [PubMed] [Google Scholar]
  10. Haselkorn R., Vogel M., Brown R. D. Conservation of the rifamycin sensitivity of transcription during T4 development. Nature. 1969 Mar 1;221(5183):836–838. doi: 10.1038/221836a0. [DOI] [PubMed] [Google Scholar]
  11. Haworth S. R., Brown L. R. Genetic analysis of ribonucleic acid polymerase mutants of Bacillus subtilis. J Bacteriol. 1973 Apr;114(1):103–113. doi: 10.1128/jb.114.1.103-113.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Heil A., Zillig W. Reconstitution of bacterial DNA-dependent RNA-polymerase from isolated subunits as a tool for the elucidation of the role of the subunits in transcription. FEBS Lett. 1970 Dec;11(3):165–168. doi: 10.1016/0014-5793(70)80519-1. [DOI] [PubMed] [Google Scholar]
  13. Hoch J. A., Barat M., Anagnostopoulos C. Transformation and transduction in recombination-defective mutants of Bacillus subtilis. J Bacteriol. 1967 Jun;93(6):1925–1937. doi: 10.1128/jb.93.6.1925-1937.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Holland M., Whiteley H. R. RNA polymerase from Bacillus amyloliquefaciens infected with phi29 bacteriophage. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2234–2237. doi: 10.1073/pnas.70.8.2234. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hussey C., Losick R., Sonenshein A. L. Ribosomal RNA synthesis is turned off during sporulation of Bacillus subtilis. J Mol Biol. 1971 Apr 14;57(1):59–70. doi: 10.1016/0022-2836(71)90119-7. [DOI] [PubMed] [Google Scholar]
  16. Hussey C., Pero J., Shorenstein R. G., Losick R. In vitro synthesis of ribosomal RNA by Bacillus subtilis RNA polymerase. Proc Natl Acad Sci U S A. 1972 Feb;69(2):407–411. doi: 10.1073/pnas.69.2.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Leighton T. J. An RNA polymerase mutation causing temperature-sensitive sporulation in Bacillus subtilis. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1179–1183. doi: 10.1073/pnas.70.4.1179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Linn T. G., Greenleaf A. L., Shorenstein R. G., Losick R. Loss of the sigma activity of RNA polymerase of Bacillus subtilis during sporulation. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1865–1869. doi: 10.1073/pnas.70.6.1865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Losick R., Sonenshein A. L. Change in the template specificity of RNA polymerase during sporulation of Bacillus subtilis. Nature. 1969 Oct 4;224(5214):35–37. doi: 10.1038/224035a0. [DOI] [PubMed] [Google Scholar]
  20. Snyder L. R. An RNA polymerase mutant of Escherichia coli defective in the T4 viral transcription program. Virology. 1972 Nov;50(2):396–403. doi: 10.1016/0042-6822(72)90391-1. [DOI] [PubMed] [Google Scholar]
  21. Sonenshein A. L., Losick R. RNA polymerase mutants blocked in sporulation. Nature. 1970 Aug 29;227(5261):906–909. doi: 10.1038/227906a0. [DOI] [PubMed] [Google Scholar]
  22. Sonenshein A. L., Roscoe D. H. The course of phage phi-e infection in sporulating cells of Bacillus subtilis strain 3610. Virology. 1969 Oct;39(2):265–275. doi: 10.1016/0042-6822(69)90047-6. [DOI] [PubMed] [Google Scholar]
  23. Stevens A. New small polypeptides associated with DNA-dependent RNA polymerase of Escherichia coli after infection with bacteriophage T4. Proc Natl Acad Sci U S A. 1972 Mar;69(3):603–607. doi: 10.1073/pnas.69.3.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sumida-Yasumoto C., Doi R. H. Transcription from the complementary deoxyribonucleic acid strands of Bacillus subtilis during various stages of sporulation. J Bacteriol. 1974 Feb;117(2):775–782. doi: 10.1128/jb.117.2.775-782.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tocchini-Valentini G. P., Marino P., Colvill A. J. Mutant of E. coli containing an altered DNA-dependent RNA polymerase. Nature. 1968 Oct 19;220(5164):275–276. doi: 10.1038/220275a0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES