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. 1974 Oct;120(1):384–389. doi: 10.1128/jb.120.1.384-389.1974

Genetic Location of Two Mutations Affecting the Lysyl-Transfer Ribonucleic Acid Synthetase of Bacillus subtilis

Francis M Racine a,1, William Steinberg a
PMCID: PMC245773  PMID: 4214413

Abstract

Two mutations (lysS1 and lysS2), each independently resulting in a thermosensitive, lysyl-transfer RNA synthetase (l-lysine: tRNA ligase [adenosine 5′-monophosphate] EC 6.1.1.6), have been mapped on the Bacillus subtilis chromosome between purA16 (adenine requirement) and sul (sulfanilamide resistance). They are linked by transformation with sul (70 to 74% cotransfer) in the order purA16-lysS1-lysS2-sul. The mutant loci are either in the same gene or in two closely linked genes. They are not linked to the tryptophanyl-tRNA synthetase structural gene or to the lys-1 locus.

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Selected References

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