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. 1974 Nov;120(2):583–589. doi: 10.1128/jb.120.2.583-589.1974

Unidirectional Growth and Branch Formation of a Morphological Mutant, Agrobacterium tumefaciens

Tamio Fujiwara a,1, Sakuzo Fukui a
PMCID: PMC245815  PMID: 4455684

Abstract

Morphological characteristics of thermoconditional mutant Agrobacterium tumefaciens F-502 were investigated in relation to growth, division, and synthesis of cellular components. As a result of a shift from 27 to 37 C, mutant cells altered their morphology from short rods to elongated and branched forms; in addition, division and deoxyribonucleic acid synthesis were inhibited at 37 C. At 37 C unidirectional cell growth and branch formation occurred at one end of a cell, and the elongation rate of a cell was proportional to cell length. A hypothetical model for branch formation is presented in which the maximal elongation rate, 1.8 μm/h, at one end of a cell is an essential factor for initiation of branch formation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler H. I., Hardigree A. A. Cell elongation in strains of Escherichia coli. J Bacteriol. 1964 May;87(5):1240–1242. doi: 10.1128/jb.87.5.1240-1242.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beachey E. H., Cole R. M. Cell wall replication in Escherichia coli, studies by immunofluorescence and immunoelectron microscopy. J Bacteriol. 1966 Oct;92(4):1245–1251. doi: 10.1128/jb.92.4.1245-1251.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Begg K. J., Donachie W. D. Topography of outer membrane growth in E. coli. Nat New Biol. 1973 Sep 12;245(141):38–39. doi: 10.1038/newbio245038a0. [DOI] [PubMed] [Google Scholar]
  4. COLLINS J. F., RICHMOND M. H. Rate of growth of Bacillus cereus between divisions. J Gen Microbiol. 1962 Apr;28:15–33. doi: 10.1099/00221287-28-1-15. [DOI] [PubMed] [Google Scholar]
  5. Cole R. M. Symposium on the fine structure and replication of bacteria and their parts. 3. Bacterial cell-wall replication followed by immunofluorescence. Bacteriol Rev. 1965 Sep;29(3):326–344. doi: 10.1128/br.29.3.326-344.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Donachie W. D., Begg K. J. Growth of the bacterial cell. Nature. 1970 Sep 19;227(5264):1220–1224. doi: 10.1038/2271220a0. [DOI] [PubMed] [Google Scholar]
  7. Ecker R. E., Kokaisl G. Synthesis of protein, ribonucleic acid, and ribosomes by individual bacterial cells in balanced growth. J Bacteriol. 1969 Jun;98(3):1219–1226. doi: 10.1128/jb.98.3.1219-1226.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Frehel C., Beaufils A. M., Ryter A. Etude au microscope électronique de la croissance de la paroi chez B. subtilis et B. megaterium. Ann Inst Pasteur (Paris) 1971 Aug;121(2):139–148. [PubMed] [Google Scholar]
  9. Fujiwara T., Fukui S. Isolation of morphological mutants of Agrobacterium tumefaciens. J Bacteriol. 1972 May;110(2):743–746. doi: 10.1128/jb.110.2.743-746.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GRULA E. A. Cell division in a species of Erwinia. VI. Growth of cells from the division end. J Bacteriol. 1962 Sep;84:599–601. doi: 10.1128/jb.84.3.599-601.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Green E. W., Schaechter M. The mode of segregation of the bacterial cell membrane. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2312–2316. doi: 10.1073/pnas.69.8.2312. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gross J. D., Karamata D., Hempstead P. G. Temperature-sensitive mutants of B. subtilis defective in DNA synthesis. Cold Spring Harb Symp Quant Biol. 1968;33:307–312. doi: 10.1101/sqb.1968.033.01.034. [DOI] [PubMed] [Google Scholar]
  13. HOWARD-FLANDERS P., SIMSON E., THERIOT L. A LOCUS THAT CONTROLS FILAMENT FORMATION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI K-12. Genetics. 1964 Feb;49:237–246. doi: 10.1093/genetics/49.2.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Helmstetter C. E. Sequence of bacterial reproduction. Annu Rev Microbiol. 1969;23:223–238. doi: 10.1146/annurev.mi.23.100169.001255. [DOI] [PubMed] [Google Scholar]
  15. Henning U., Rehn K., Hoehn B. Cell envelope and shape of Escherichia coli K12. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2033–2036. doi: 10.1073/pnas.70.7.2033. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hirota Y., Ryter A., Jacob F. Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division. Cold Spring Harb Symp Quant Biol. 1968;33:677–693. doi: 10.1101/sqb.1968.033.01.077. [DOI] [PubMed] [Google Scholar]
  17. Inouye M. Unlinking of cell division from deoxyribonucleic acid replication in a temperature-sensitive deoxyribonucleic acid synthesis mutant of Escherichia coli. J Bacteriol. 1969 Sep;99(3):842–850. doi: 10.1128/jb.99.3.842-850.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. KECK K. An ultramicro technique for the determination of deoxypentose nucleic acid. Arch Biochem Biophys. 1956 Aug;63(2):446–451. doi: 10.1016/0003-9861(56)90059-5. [DOI] [PubMed] [Google Scholar]
  19. Karamata D., McConnell M., Rogers H. J. Mapping of rod mutants of Bacillus subtilis. J Bacteriol. 1972 Jul;111(1):73–79. doi: 10.1128/jb.111.1.73-79.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Kojima M., Suda S., Hotta S., Hamada K. Induction of pleomorphy and calcium ion deficiency in Lactobacillus bifidus. J Bacteriol. 1970 Apr;102(1):217–220. doi: 10.1128/jb.102.1.217-220.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Krulwich T. A., Ensign J. C., Tipper D. J., Strominger J. L. Sphere-rod morphogenesis in Arthrobacter crystallopoietes. I. Cell wall composition and polysaccharides of the peptidoglycan. J Bacteriol. 1967 Sep;94(3):734–740. doi: 10.1128/jb.94.3.734-740.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Kubitschek H. E. Control of cell growth in bacteria: experiments with thymine starvation. J Bacteriol. 1971 Feb;105(2):472–476. doi: 10.1128/jb.105.2.472-476.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Kubitschek H. E. Growth during the bacterial cell cycle: analysis of cell size distribution. Biophys J. 1969 Jun;9(6):792–809. doi: 10.1016/S0006-3495(69)86418-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. LARK C., LARK K. G. The effects of D-amino acids on Alcaligenes fecalis. Can J Microbiol. 1959 Aug;5:369–379. doi: 10.1139/m59-046. [DOI] [PubMed] [Google Scholar]
  25. Leighton P. M., Donachie W. D. Deoxyribonucleic acid synthesis and cell division in a lon- mutant of Escherichia coli. J Bacteriol. 1970 Jun;102(3):810–814. doi: 10.1128/jb.102.3.810-814.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Lin E. C., Hirota Y., Jacob F. On the process of cellular division in Escherichia coli. VI. Use of a methocel-autoradiographic method for the study of cellular division in Escherichia coli. J Bacteriol. 1971 Oct;108(1):375–385. doi: 10.1128/jb.108.1.375-385.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Matsuzawa H., Hayakawa K., Sato T., Imahori K. Characterization and genetic analysis of a mutant of Escherichia coli K-12 with rounded morphology. J Bacteriol. 1973 Jul;115(1):436–442. doi: 10.1128/jb.115.1.436-442.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Mauck J., Chan L., Glaser L., Williamson J. Mode of cell wall growth of Bacillus megaterium. J Bacteriol. 1972 Jan;109(1):373–378. doi: 10.1128/jb.109.1.373-378.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Mauck J., Glaser L. On the mode of in vivo assembly of the cell wall of Bacillus subtilis. J Biol Chem. 1972 Feb 25;247(4):1180–1187. [PubMed] [Google Scholar]
  30. Mendelson N. H., Gross J. D. Characterization of a temperature-sensitive mutant of Bacillus subtilis defective in deoxyribonucleic acid replication. J Bacteriol. 1967 Nov;94(5):1603–1608. doi: 10.1128/jb.94.5.1603-1608.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Reeve J. N., Mendelson N. H. Cell morphology of Bacillus subtilis: the effect of genetic background on the expression of a rod - gene. Mol Gen Genet. 1972;119(1):11–26. doi: 10.1007/BF00270440. [DOI] [PubMed] [Google Scholar]
  32. Ryter A. Etude de la croissance de la membrane chez Bacillus subtilis au moyen de la distribution des flagelles. Ann Inst Pasteur (Paris) 1971 Sep;121(3):271–288. [PubMed] [Google Scholar]
  33. Ryter A., Hirota Y., Schwarz U. Process of cellular division in Escherichia coli growth pattern of E. coli murein. J Mol Biol. 1973 Jun 25;78(1):185–195. doi: 10.1016/0022-2836(73)90437-3. [DOI] [PubMed] [Google Scholar]
  34. Suit J. C., Barbee T., Jetton S. Morphological changes in Escherichia coli strain C produced by treatments affecting deoxyribonucleic acid synthesis. J Gen Microbiol. 1967 Oct;49(1):165–173. doi: 10.1099/00221287-49-1-165. [DOI] [PubMed] [Google Scholar]
  35. Tsukagoshi N., Fielding P., Fox C. F. Membrane assembly in Escherichia coli. I. Segregation of preformed and newly formed membrane into daughter cells. Biochem Biophys Res Commun. 1971 Jul 16;44(2):497–502. doi: 10.1016/0006-291x(71)90629-2. [DOI] [PubMed] [Google Scholar]
  36. Weinbaum G. Characteristics of cell walls from morphological variants of Escherichia coli. J Gen Microbiol. 1966 Jan;42(1):83–92. doi: 10.1099/00221287-42-1-83. [DOI] [PubMed] [Google Scholar]
  37. Zusman D., Gottlieb P., Rosenberg E. Division cycle of Myxococcus xanthus. 3. Kinetics of cell growth and protein synthesis. J Bacteriol. 1971 Mar;105(3):811–819. doi: 10.1128/jb.105.3.811-819.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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