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. 1974 Nov;120(2):955–964. doi: 10.1128/jb.120.2.955-964.1974

Isolation and Characterization of Bacteria That Grow on Methane and Organic Compounds as Sole Sources of Carbon and Energy

Tom E Patt 1, Gloria C Cole 1, Judith Bland 1, R S Hanson 1
PMCID: PMC245862  PMID: 4142033

Abstract

Bacteria capable of growth on methane and a variety of complex organic substrates as sole sources of carbon and energy have been isolated. Conditions used to rigorously establish the purity of the cultures are described. One facultative methylotroph has been studied in detail. This organism has peripherally arranged pairs of intracytoplasmic membranes characteristic of obligate methylotrophs. This isolate apparently utilizes the serine pathway of formaldehyde fixation. The location of methane oxidizers in a dimictic lake indicates that these organisms prefer less than saturating levels of dissolved oxygen. Laboratory experiments confirmed the preference of these organisms for atmospheres containing less oxygen than air.

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Selected References

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  1. BROWN L. R., STRAWINSKI R. J., MCCLESKEY C. S. THE ISOLATION AND CHARACTERIZATION OF METHANOMONAS METHANOOXIDANS BROWN AND STRAWINSKI. Can J Microbiol. 1964 Oct;10:791–799. doi: 10.1139/m64-100. [DOI] [PubMed] [Google Scholar]
  2. Banerjee S., Fraenkel D. G. Glucose-6-phosphate dehydrogenase from Escherichia coli and from a "high-level" mutant. J Bacteriol. 1972 Apr;110(1):155–160. doi: 10.1128/jb.110.1.155-160.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CONTI S. F., HIRSCH P. BIOLOGY OF BUDDING BACTERIA. 3. FINE STRUCTURE OF RHODOMICROBIUM AND HYPHOMICROBIUM SPP. J Bacteriol. 1965 Feb;89:503–512. doi: 10.1128/jb.89.2.503-512.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Colby J., Zatman L. J. Hexose phosphate synthese and tricarboxylic acid-cycle enzymes in bacterium 4B6, an obligate methylotroph. Biochem J. 1972 Aug;128(5):1373–1376. doi: 10.1042/bj1281373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DEVAY J. E., SCHNATHORST W. C. SINGLE-CELL ISOLATION AND PRESERVATION OF BACTERIAL CULTURES. Nature. 1963 Aug 24;199:775–777. doi: 10.1038/199775a0. [DOI] [PubMed] [Google Scholar]
  6. DWORKIN M., FOSTER J. W. Studies on Pseudomonas methanica (Söhngen) nov. comb. J Bacteriol. 1956 Nov;72(5):646–659. doi: 10.1128/jb.72.5.646-659.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Davey J. F., Whittenbury R., Wilkinson J. F. The distribution in the methylobacteria of some key enzymes concerned with intermediary metabolism. Arch Mikrobiol. 1972;87(4):359–366. doi: 10.1007/BF00409135. [DOI] [PubMed] [Google Scholar]
  8. Davies S. L., Whittenbury R. Fine structure of methane and other hydrocarbon-utilizing bacteria. J Gen Microbiol. 1970 May;61(2):227–232. doi: 10.1099/00221287-61-2-227. [DOI] [PubMed] [Google Scholar]
  9. Foster J. W., Davis R. H. A methane-dependent coccus, with notes on classification and nomenclature of obligate, methane-utilizing bacteria. J Bacteriol. 1966 May;91(5):1924–1931. doi: 10.1128/jb.91.5.1924-1931.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Freese E., Klofat W., Galliers E. Commitment to sporulation and induction of glucose-phosphoenolpyruvate-transferase. Biochim Biophys Acta. 1970 Nov 24;222(2):265–289. doi: 10.1016/0304-4165(70)90115-7. [DOI] [PubMed] [Google Scholar]
  11. Harder W., Quayle J. R. Aspects of glycine and serine biosynthesis during growth of Pseudomonas AM1 on C compounds. Biochem J. 1971 Mar;121(5):763–769. doi: 10.1042/bj1210763. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Harder W., Quayle J. R. The biosynthesis of serine and glycine in Pseudomonas AM1 with special reference to growth on carbon sources other than C1 compounds. Biochem J. 1971 Mar;121(5):753–762. doi: 10.1042/bj1210753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. KANEDA T., ROXBURGH J. M. Serine as an intermediate in the assimilation of methanol by a Pseudomonas. Biochim Biophys Acta. 1959 May;33(1):106–110. doi: 10.1016/0006-3002(59)90503-7. [DOI] [PubMed] [Google Scholar]
  14. LARGE P. J., PEEL D., QUAYLE J. R. Microbial growth on C1 compounds. II. Synthesis of cell constituents by methanol- and formate-grown Pseudomonas AM 1, and methanol-grown Hyphomicrobium vulgare. Biochem J. 1961 Dec;81:470–480. doi: 10.1042/bj0810470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Large P. J., Quayle J. R. Microbial growth on C(1) compounds. 5. Enzyme activities in extracts of Pseudomonas AM1. Biochem J. 1963 May;87(2):386–396. doi: 10.1042/bj0870386. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lawrence A. J., Kemp M. B., Quayle J. R. Synthesis of cell constituents by methane-grown Methylococcus capsulatus and Methanomonas methanooxidans. Biochem J. 1970 Feb;116(4):631–639. doi: 10.1042/bj1160631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lawrence A. J., Quayle J. R. Alternative carbon assimilation pathways in methane-utilizing bacteria. J Gen Microbiol. 1970 Nov;63(3):371–374. doi: 10.1099/00221287-63-3-371. [DOI] [PubMed] [Google Scholar]
  19. McFadden B. A., Denend A. R. Ribulose diphosphate carboxylase from autotrophic microorganisms. J Bacteriol. 1972 May;110(2):633–642. doi: 10.1128/jb.110.2.633-642.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. RYTER A., KELLENBERGER E., BIRCHANDERSEN A., MAALOE O. Etude au microscope électronique de plasmas contenant de l'acide désoxyribonucliéique. I. Les nucléoides des bactéries en croissance active. Z Naturforsch B. 1958 Sep;13B(9):597–605. [PubMed] [Google Scholar]
  21. Ribbons D. W., Harrison J. E., Wadzinski A. M. Metabolism of single carbon compounds. Annu Rev Microbiol. 1970;24:135–158. doi: 10.1146/annurev.mi.24.100170.001031. [DOI] [PubMed] [Google Scholar]
  22. SCHILDKRAUT C. L., MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol. 1962 Jun;4:430–443. doi: 10.1016/s0022-2836(62)80100-4. [DOI] [PubMed] [Google Scholar]
  23. SZYBALSKI W., BRYSON V. Genetic studies on microbial cross resistance to toxic agents. I. Cross resistance of Escherichia coli to fifteen antibiotics. J Bacteriol. 1952 Oct;64(4):489–499. doi: 10.1128/jb.64.4.489-499.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Spurr A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969 Jan;26(1):31–43. doi: 10.1016/s0022-5320(69)90033-1. [DOI] [PubMed] [Google Scholar]
  25. Stieglitz B., Mateles R. I. Methanol metabolism in pseudomonad C. J Bacteriol. 1973 Apr;114(1):390–398. doi: 10.1128/jb.114.1.390-398.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Whittenbury R., Phillips K. C., Wilkinson J. F. Enrichment, isolation and some properties of methane-utilizing bacteria. J Gen Microbiol. 1970 May;61(2):205–218. doi: 10.1099/00221287-61-2-205. [DOI] [PubMed] [Google Scholar]

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