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. 1974 Dec;120(3):1356–1363. doi: 10.1128/jb.120.3.1356-1363.1974

Isolation of Membrane-Associated Folded Chromosomes from Escherichia coli: Effect of Protein Synthesis Inhibition

Oliver A Ryder 1, Douglas W Smith 1
PMCID: PMC245922  PMID: 4612018

Abstract

The sedimentation properties of membrane-associated folded chromosomes prepared from Escherichia coli TAU-bar at 0 to 4 C were studied. Utilizing a modification of the procedure of Stonington and Pettijohn (1971), quantitative yields of membrane-associated folded chromosomes may be obtained. Folded chromosomes remained associated with the cell envelope during their replication and after completion of residual synthesis in the absence of required amino acids, as demonstrated by sedimentation velocities and the presence of high levels of cosedimenting protein. Membrane-associated folded chromosomes isolated from amino acid-starved cells sedimented more rapidly than membrane-associated folded chromosomes isolated from exponentially growing cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Billen D., Hewitt R. Influence of starvation for methionine and other amino acids on subsequent bacterial deoxyribonucleic acid replication. J Bacteriol. 1966 Sep;92(3):609–617. doi: 10.1128/jb.92.3.609-617.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bird R., Lark K. G. Initiation and termination of DNA replication after amino acid starvation of E. coli 15T-. Cold Spring Harb Symp Quant Biol. 1968;33:799–808. doi: 10.1101/sqb.1968.033.01.092. [DOI] [PubMed] [Google Scholar]
  3. CUMMINGS D. J. SEDIMENTATION AND BIOLOGICAL PROPERTIES OF T-PHAGES OF ESCHERICHIA COLI. Virology. 1964 Jul;23:408–418. doi: 10.1016/0042-6822(64)90264-8. [DOI] [PubMed] [Google Scholar]
  4. Carl P. L. Escherichia coli mutants with temperature-sensitive synthesis of DNA. Mol Gen Genet. 1970;109(2):107–122. doi: 10.1007/BF00269647. [DOI] [PubMed] [Google Scholar]
  5. Caro L., Berg C. M. Chromosome replication in Escherichia coli. II. Origin of replication in F- and F+ strains. J Mol Biol. 1969 Oct 28;45(2):325–336. doi: 10.1016/0022-2836(69)90108-9. [DOI] [PubMed] [Google Scholar]
  6. DAVIS J. E., SINSHEIMER R. L. The replication of bacteriophage MS2. 1. Transfer of parental nucleic acid to progeny phage. J Mol Biol. 1963 Mar;6:203–207. doi: 10.1016/s0022-2836(63)80069-8. [DOI] [PubMed] [Google Scholar]
  7. Delius H., Worcel A. Letter: Electron microscopic visualization of the folded chromosome of Escherichia coli. J Mol Biol. 1974 Jan 5;82(1):107–109. doi: 10.1016/0022-2836(74)90577-4. [DOI] [PubMed] [Google Scholar]
  8. Dworsky P., Schaechter M. Effect of rifampin on the structure and membrane attachment of the nucleoid of Escherichia coli. J Bacteriol. 1973 Dec;116(3):1364–1374. doi: 10.1128/jb.116.3.1364-1374.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HANAWALT P. C. Involvement of synthesis of RNA in thymineless death. Nature. 1963 Apr 20;198:286–286. doi: 10.1038/198286a0. [DOI] [PubMed] [Google Scholar]
  10. Hirota Y., Mordoh J., Jacob F. On the process of cellular division in Escherichia coli. 3. Thermosensitive mutants of Escherichia coli altered in the process of DNA initiation. J Mol Biol. 1970 Nov 14;53(3):369–387. doi: 10.1016/0022-2836(70)90072-0. [DOI] [PubMed] [Google Scholar]
  11. Kuempel P. L. Temperature-sensitive initiation of chromosome replication in a mutant of Escherichia coli. J Bacteriol. 1969 Dec;100(3):1302–1310. doi: 10.1128/jb.100.3.1302-1310.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LARK C., LARK K. G. EVIDENCE FOR TWO DISTINCT ASPECTS OF THE MECHANISM REGULATING CHROMOSOME REPLICATION IN ESCHERICHIA COLI. J Mol Biol. 1964 Oct;10:120–136. doi: 10.1016/s0022-2836(64)80032-2. [DOI] [PubMed] [Google Scholar]
  13. LARK K. G., REPKO T., HOFFMAN E. J. THE EFFECT OF AMINO ACID DEPRIVATION ON SUBSEQUENT DEOXYRIBONUCLEIC ACID REPLICATION. Biochim Biophys Acta. 1963 Sep 17;76:9–24. [PubMed] [Google Scholar]
  14. MAALOE O., HANAWALT P. C. Thymine deficiency and the normal DNA replication cycle. I. J Mol Biol. 1961 Apr;3:144–155. doi: 10.1016/s0022-2836(61)80041-7. [DOI] [PubMed] [Google Scholar]
  15. Marunouchi T., Messer W. Replication of a specific terminal chromosome segment in Escherichia coli which is required for cell division. J Mol Biol. 1973 Jun 25;78(1):211–228. doi: 10.1016/0022-2836(73)90439-7. [DOI] [PubMed] [Google Scholar]
  16. Morgan C., Rosenkranz H. S., Carr H. S., Rose H. M. Electron microscopy of chloramphenicol-treated Escherichia coli. J Bacteriol. 1967 Jun;93(6):1987–2002. doi: 10.1128/jb.93.6.1987-2002.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schubach W. H., Whitmer J. D., Davern C. I. Genetic control of DNA initiation in Escherichia coli. J Mol Biol. 1973 Feb 25;74(2):205–221. doi: 10.1016/0022-2836(73)90107-1. [DOI] [PubMed] [Google Scholar]
  18. Stonington O. G., Pettijohn D. E. The folded genome of Escherichia coli isolated in a protein-DNA-RNA complex. Proc Natl Acad Sci U S A. 1971 Jan;68(1):6–9. doi: 10.1073/pnas.68.1.6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wechsler J. A., Gross J. D. Escherichia coli mutants temperature-sensitive for DNA synthesis. Mol Gen Genet. 1971;113(3):273–284. doi: 10.1007/BF00339547. [DOI] [PubMed] [Google Scholar]
  20. Worcel A., Burgi E. On the structure of the folded chromosome of Escherichia coli. J Mol Biol. 1972 Nov 14;71(2):127–147. doi: 10.1016/0022-2836(72)90342-7. [DOI] [PubMed] [Google Scholar]

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