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. 1975 Mar;121(3):857–862. doi: 10.1128/jb.121.3.857-862.1975

Integration of R plasmid Rts1 to the gal region of the Escherichia coli chromosome.

Y Terawaki, H Kishi, R Nakaya
PMCID: PMC246012  PMID: 1090604

Abstract

An R plasmid Rts1 was integrated into the gal region of the chromosome of Escherichia coli XA-7012 (galE) strain by the directed transposition technique. The integration of the Rts1 genome was confirmed mainly by conjugation studies and also by transduction experiments using phage P1. As a result, it was found that the integrated genome contained genes responsible for kanamycin resistance, conjugal transferability, and for autonomous replication. As reported previously, Rts1 is temperature sensitive in replication and inhibits the growth of the host at nonpermissive temperature. However, although a plasmid derived from the integrated Rts1 genome still demonstrates temperature sensitivity upon transfer and high level of kanamycin resistance, this plasmid no longer displays temperature sensitivity in replication and the inhibitory effect on the host. These results indicate that the temperature sensitivity of replication of Rts1 and its inhibitory effect on the host cell are due to the presence of a gene or gene cluster on the Rts1 genome and that the gene(s) is clearly discriminated from the one responsible for the temperature sensitivity of transfer.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Coetzee J. N., Datta N., Hedges R. W. R factors from Proteus rettgeri. J Gen Microbiol. 1972 Oct;72(3):543–552. doi: 10.1099/00221287-72-3-543. [DOI] [PubMed] [Google Scholar]
  2. DiJoseph C. G., Bayer M. E., Kaji A. Host cell growth in the presence of the thermosensitive drug resistance factor, Rts1. J Bacteriol. 1973 Jul;115(1):399–410. doi: 10.1128/jb.115.1.399-410.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DiJoseph C. G. The thermosensitive lesion in the replication of the drug resistance factor, Rts1. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2515–2519. doi: 10.1073/pnas.71.6.2515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Goto N., Yoshida Y., Terawaki Y., Nakaya R., Suzuki K. Base composition of deoxyribonucleic acid of the temperature-sensitive kanamycin-resistant R factor, Rts1. J Bacteriol. 1970 Mar;101(3):856–859. doi: 10.1128/jb.101.3.856-859.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ippen K., Shapiro J. A., Beckwith J. R. Transposition of the lac region to the gal region of the Escherichia coli chromosome: isolation of lambda-lac transducing bacteriophages. J Bacteriol. 1971 Oct;108(1):5–9. doi: 10.1128/jb.108.1.5-9.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Iyobe S., Hashimoto H., Mitsuhashi S. Integration of chloramphenicol resistance gene of an R factor on Escherichia coli chromosome. Jpn J Microbiol. 1969 Sep;13(3):225–232. doi: 10.1111/j.1348-0421.1969.tb00461.x. [DOI] [PubMed] [Google Scholar]
  7. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  8. Moody E. E., Runge R. The integration of autonomous transmissible plasmids into the chromosome of Escherichia coli K12. Genet Res. 1972 Apr;19(2):181–186. doi: 10.1017/s0016672300014427. [DOI] [PubMed] [Google Scholar]
  9. Nishimura A., Nishimura Y., Caro L. Isolation of Hfr strains from R+ and ColV2+ strains of Escherichia coli and derivation of an R'lac factor by transduction. J Bacteriol. 1973 Dec;116(3):1107–1112. doi: 10.1128/jb.116.3.1107-1112.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nishimura Y., Caro L., Berg C. M., Hirota Y. Chromosome replication in Escherichia coli. IV. Control of chromosome replication and cell division by an integrated episome. J Mol Biol. 1971 Feb 14;55(3):441–456. doi: 10.1016/0022-2836(71)90328-7. [DOI] [PubMed] [Google Scholar]
  11. Rownd R. Replication of a bacterial episome under relaxed control. J Mol Biol. 1969 Sep 28;44(3):387–402. doi: 10.1016/0022-2836(69)90368-4. [DOI] [PubMed] [Google Scholar]
  12. Terawaki Y., Kakizawa Y., Takayasu H., Yoshikawa M. Temperature sensitivity of cell growth in Escherichia coli associated with the temperature sensitive R(KM) factor. Nature. 1968 Jul 20;219(5151):284–285. doi: 10.1038/219284a0. [DOI] [PubMed] [Google Scholar]
  13. Terawaki Y., Rownd R. Replication of the R factor Rts1 in Proteus mirabilis. J Bacteriol. 1972 Feb;109(2):492–498. doi: 10.1128/jb.109.2.492-498.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Terawaki Y., Takayasu H., Akiba T. Thermosensitive replication of a kanamycin resistance factor. J Bacteriol. 1967 Sep;94(3):687–690. doi: 10.1128/jb.94.3.687-690.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Yokota T., Kanamaru Y., Mori R., Akiba T. Recombination between a thermosensitive kanamycin resistance factor and a nonthermosensitive multiple-drug resistant factor. J Bacteriol. 1969 Jun;98(3):863–873. doi: 10.1128/jb.98.3.863-873.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Yoshikawa M. Identification and mapping of the replication genes of an R factor, R100-1, integrated into the chromosome of Escherichia coli K-12. J Bacteriol. 1974 Jun;118(3):1123–1131. doi: 10.1128/jb.118.3.1123-1131.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

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