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. 1975 Mar;121(3):1064–1073. doi: 10.1128/jb.121.3.1064-1073.1975

Kinetics of induced and repressed enzyme synthesis in Saccharomyces cerevisiae.

R P Lawther, T G Cooper
PMCID: PMC246036  PMID: 1090586

Abstract

Our previous work has shown that both induction, after addition of inducer, and loss of ability to produce allophanate hydrolase, after removal of inducer, proceed more rapidly than expected from the reported half-life of messenger ribonucleic acid in Saccharomyces cerevisiae. As a basis of rectifying these observations, we have characterized induction and repression of allophanate hydrolase synthesis and find that: (i) induction of the hydrolase begins immediately upon addition of inducer, (ii) once induction has been initiated removal of inducer does not result in immediate loss of synthetic capacity, (iii) induction of the capacity to produce hydrolase can occur in the absence of protein synthesis, (iv) the half-life of hydrolase synthetic capacity increases if protein synthesis is inhibited, (v) allophanate hydrolase itself is not degraded upon removal of inducer, and (vi) induction and repression of allophanate hydrolase synthetic capacity likely occurs at the level of transcription.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bossinger J., Lawther R. P., Cooper T. G. Nitrogen repression of the allantoin degradative enzymes in Saccharomyces cerevisiae. J Bacteriol. 1974 Jun;118(3):821–829. doi: 10.1128/jb.118.3.821-829.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cooper T. G., Lawther R. P. Induction of the allantoin degradative enzymes in Saccharomyces cerevisiae by the last intermediate of the pathway. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2340–2344. doi: 10.1073/pnas.70.8.2340. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cooper T. G., Sumrada R. Urea transport in Saccharomyces cerevisiae. J Bacteriol. 1975 Feb;121(2):571–576. doi: 10.1128/jb.121.2.571-576.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cybis J., Weglenski P. Arginase induction in Aspergillus nidulans. The appearance and decay of the coding capacity of messenger. Eur J Biochem. 1972 Oct;30(2):262–268. doi: 10.1111/j.1432-1033.1972.tb02094.x. [DOI] [PubMed] [Google Scholar]
  5. Darnell J. E., Wall R., Tushinski R. J. An adenylic acid-rich sequence in messenger RNA of HeLa cells and its possible relationship to reiterated sites in DNA. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1321–1325. doi: 10.1073/pnas.68.6.1321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Edmonds M., Vaughan M. H., Jr, Nakazato H. Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1336–1340. doi: 10.1073/pnas.68.6.1336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Greenberg J. R., Perry R. P. Relative occurrence of polyadenylic acid sequences in messenger and heterogeneous nuclear RNA of L cells as determined by poly (U)-hydroxylapatite chromatography. J Mol Biol. 1972 Dec 14;72(1):91–98. doi: 10.1016/0022-2836(72)90070-8. [DOI] [PubMed] [Google Scholar]
  8. HARTWELL L. H., MAGASANIK B. THE MOLECULAR BASIS OF HISTIDASE INDUCTION IN BACILLUS SUBTILIS. J Mol Biol. 1963 Oct;7:401–420. doi: 10.1016/s0022-2836(63)80033-9. [DOI] [PubMed] [Google Scholar]
  9. Hartwell L. H., Hutchison H. T., Holland T. M., McLaughlin C. S. The effect of cycloheximide upon polyribosome stability in two yeast mutants defective respectively in the initiation of polypeptide chains and in messenger RNA synthesis. Mol Gen Genet. 1970;106(4):347–361. doi: 10.1007/BF00324052. [DOI] [PubMed] [Google Scholar]
  10. Hutchison H. T., Hartwell L. H., McLaughlin C. S. Temperature-sensitive yeast mutant defective in ribonucleic acid production. J Bacteriol. 1969 Sep;99(3):807–814. doi: 10.1128/jb.99.3.807-814.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jacobson A., Firtel R. A., Lodish H. Transcription of polydeoxythymidylate sequences in the genome of the cellular slime mold, Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1974 May;71(5):1607–1611. doi: 10.1073/pnas.71.5.1607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. KEPES A. KINETICS OF INDUCED ENZYME SYNTHESIS. DETERMINATION OF THE MEAN LIFE OF GALACTOSIDASE-SPECIFIC MESSENGER RNA. Biochim Biophys Acta. 1963 Oct 15;76:293–309. [PubMed] [Google Scholar]
  13. Klo S. C., Cano F. R., Lampen J. O. Lomofungin, an inhibitor of ribonucleic acid synthesis in yeast protoplasts: its effect on enzyme formation. Antimicrob Agents Chemother. 1973 Jun;3(6):716–722. doi: 10.1128/aac.3.6.716. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lawther R. P., Cooper T. G. Effects of inducer addition and removal upon the level of allophanate hydrolase in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1973 Dec 19;55(4):1100–1104. doi: 10.1016/s0006-291x(73)80008-7. [DOI] [PubMed] [Google Scholar]
  15. Lee S. Y., Mendecki J., Brawerman G. A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1331–1335. doi: 10.1073/pnas.68.6.1331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schimke R. T., Doyle D. Control of enzyme levels in animal tissues. Annu Rev Biochem. 1970;39:929–976. doi: 10.1146/annurev.bi.39.070170.004433. [DOI] [PubMed] [Google Scholar]
  17. Singer R. H., Penman S. Messenger RNA in HeLa cells: kinetics of formation and decay. J Mol Biol. 1973 Aug 5;78(2):321–334. doi: 10.1016/0022-2836(73)90119-8. [DOI] [PubMed] [Google Scholar]
  18. Tonnesen T., Friesen J. D. Inhibitors of ribonucleic acid synthesis in Saccharomyces cerevisiae: decay rate of messenger ribonucleic acid. J Bacteriol. 1973 Sep;115(3):889–896. doi: 10.1128/jb.115.3.889-896.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wei C. M., Hansen B. S., Vaughan M. H., Jr, McLaughlin C. S. Mechanism of action of the mycotoxin trichodermin, a 12,13-epoxytrichothecene. Proc Natl Acad Sci U S A. 1974 Mar;71(3):713–717. doi: 10.1073/pnas.71.3.713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wei C. M., McLaughlin C. S. Structure-function relationship in the 12,13-epoxytrichothecenes. Novel inhibitors of protein synthesis. Biochem Biophys Res Commun. 1974 Apr 8;57(3):838–844. doi: 10.1016/0006-291x(74)90622-6. [DOI] [PubMed] [Google Scholar]
  21. Whitney P. A., Cooper T. G. Urea carboxylase and allophanate hydrolase. Two components of adenosine triphosphate:urea amido-lyase in Saccharomyces cerevisiae. J Biol Chem. 1972 Mar 10;247(5):1349–1353. [PubMed] [Google Scholar]

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