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. 1975 Jun;122(3):847–854. doi: 10.1128/jb.122.3.847-854.1975

Dominant and semidominant mutations leading to thermosensitivity of ribonucleic acid biosynthesis in Saccharomyces cerevisiae.

F Lacroute, J Huet, F Exinger
PMCID: PMC246134  PMID: 1097402

Abstract

Different dominant thermosensitive mutations affecting the same gene were selected in Saccharomyces cerevisiae. Ribonucleic acid (RNA) synthesis decreased rapidly and markedly at 37 C in all the mutants whether they were in a homozygous or a heterozygous state. Protein biosynthesis was at first unaffected and then decreased slowly, stopping after 5 h. Measurements of RNA biosynthesis in isolated nuclei as well as in vitro activities of RNA polymerases A and B at 22 and 37 C failed to reveal any difference between mutants and the wild type. Analysis of the nature of the residual RNAs synthesized at the high temperature in the mutants showed a small relative increase in the messenger RNA fraction, but it was not sufficient to indicate a specific inactivation of RNA polymerase A activity. The results suggest an impairment in a common regulatory element for all RNA polymerases acting at the level of the initiation of transcription. Similar mutants with a semidominant phenotype were obtained in which the lesions were in two other unlinked loci.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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