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. 1973 Sep;115(3):1077–1083. doi: 10.1128/jb.115.3.1077-1083.1973

Ethidium Bromide-Resistant Mutant of Bacillus subtilis

Paul E Bishop 1,2, Lyle R Brown 1,2
PMCID: PMC246356  PMID: 4199505

Abstract

An ethidium bromide-resistant mutant (EB8) derived from a Marburg strain of Bacillus subtilis was found to be conditionally resistant to 10 μg of ethidium bromide per ml. Expression of resistance is complete only during vegetative growth at incubation temperatures above 30 C in complex medium or minimal medium supplemented with Casamino Acids. Strain EB8 is cross-resistant to acriflavine and proflavine. The ethidium bromide resistance marker is co-transduced with hisA1 at a frequency of 6% and is located to the right of hisA1 on the B. subtilis chromosome as it is usually represented on the map. Incorporation of [5-3H] uridine by strain EB8 showed that ribonucleic acid synthesis in both whole cells and protoplasts is ethidium bromide-resistant.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anagnostopoulos C., Spizizen J. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS. J Bacteriol. 1961 May;81(5):741–746. doi: 10.1128/jb.81.5.741-746.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bouanchaud D. H., Scavizzi M. R., Chabbert Y. A. Elimination by ethidium bromide of antibiotic resistance in enterobacteria and staphylococci. J Gen Microbiol. 1968 Dec;54(3):417–425. doi: 10.1099/00221287-54-3-417. [DOI] [PubMed] [Google Scholar]
  3. Boylan R. J., Mendelson N. H., Brooks D., Young F. E. Regulation of the bacterial cell wall: analysis of a mutant of Bacillus subtilis defective in biosynthesis of teichoic acid. J Bacteriol. 1972 Apr;110(1):281–290. doi: 10.1128/jb.110.1.281-290.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clive D., Landman O. E. Reversion of Bacillus subtilis protoplasts to the bacillary form induced by exogenous cell wall, bacteria and by growth in membrane filters. J Gen Microbiol. 1970 May;61(2):233–243. doi: 10.1099/00221287-61-2-233. [DOI] [PubMed] [Google Scholar]
  5. Dubnau D., Goldthwaite C., Smith I., Marmur J. Genetic mapping in Bacillus subtilis. J Mol Biol. 1967 Jul 14;27(1):163–185. doi: 10.1016/0022-2836(67)90358-0. [DOI] [PubMed] [Google Scholar]
  6. Gitler C., Rubalcava B., Caswell A. Fluorescence changes of ethidium bromide on binding to erythrocyte and mitochondrial membranes. Biochim Biophys Acta. 1969;193(2):479–481. doi: 10.1016/0005-2736(69)90208-9. [DOI] [PubMed] [Google Scholar]
  7. Grant D. J. Inhibitory effects of ethidium bromide on the growth kinetics of Klebsiella aerogenes, adaptation to ethidium and cross-resistance to proflavine. Antonie Van Leeuwenhoek. 1969;35(4):479–496. doi: 10.1007/BF02219166. [DOI] [PubMed] [Google Scholar]
  8. Grant G. F., Simon M. I. Synthesis of bacterial flagella. II. PBS1 transduction of flagella-specific markers in Bacillus subtilis. J Bacteriol. 1969 Jul;99(1):116–124. doi: 10.1128/jb.99.1.116-124.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Haworth S. R., Brown L. R. Genetic analysis of ribonucleic acid polymerase mutants of Bacillus subtilis. J Bacteriol. 1973 Apr;114(1):103–113. doi: 10.1128/jb.114.1.103-113.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hoch J. A., Barat M., Anagnostopoulos C. Transformation and transduction in recombination-defective mutants of Bacillus subtilis. J Bacteriol. 1967 Jun;93(6):1925–1937. doi: 10.1128/jb.93.6.1925-1937.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LERMAN L. S. The structure of the DNA-acridine complex. Proc Natl Acad Sci U S A. 1963 Jan 15;49:94–102. doi: 10.1073/pnas.49.1.94. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Landman O. E., Forman A. Gelatin-induced reversion of protoplasts of Bacillus subtilis to the bacillary form: biosynthesis of macromolecules and wall during successive steps. J Bacteriol. 1969 Aug;99(2):576–589. doi: 10.1128/jb.99.2.576-589.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Maillet S. Action mutagène spécifique de trois colorants, sur la mutation reverse d'un gène ProA chez E. coli K 12. C R Acad Sci Hebd Seances Acad Sci D. 1969 Oct 29;269(17):1708–1711. [PubMed] [Google Scholar]
  14. Nakamura H., Suganuma A. Membrane mutation associated with sensitivity to acriflavine in Escherichia coli. J Bacteriol. 1972 Apr;110(1):329–335. doi: 10.1128/jb.110.1.329-335.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Paoletti C., Couder H., Guerineau M. A yeast mitochondrial deoxyribonuclease stimulated by ethidium bromide. Biochem Biophys Res Commun. 1972 Aug 21;48(4):950–958. doi: 10.1016/0006-291x(72)90701-2. [DOI] [PubMed] [Google Scholar]
  16. Rubin S. J., Rosenblum E. D. Effects of ethidium bromide on growth and on loss of the penicillinase plasmid of Staphylococcus aureus. J Bacteriol. 1971 Dec;108(3):1200–1204. doi: 10.1128/jb.108.3.1200-1204.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. SACKS L. E., ALDERTON G. Behavior of bacterial spores in aqueous polymer two-phase systems. J Bacteriol. 1961 Sep;82:331–341. doi: 10.1128/jb.82.3.331-341.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Saffhill R., Schneider-Bernloehr H., Orgel L. E., Spiegelman S. In vitro selection of bacteriophage Q-beta ribonucleic acid variants resistant to ethidium bromide. J Mol Biol. 1970 Aug;51(3):531–539. doi: 10.1016/0022-2836(70)90006-9. [DOI] [PubMed] [Google Scholar]
  19. Slonimski P. P., Perrodin G., Croft J. H. Ethidium bromide induced mutation of yeast mitochondria: complete transformation of cells into respiratory deficient non-chromosomal "petites". Biochem Biophys Res Commun. 1968 Feb 15;30(3):232–239. doi: 10.1016/0006-291x(68)90440-3. [DOI] [PubMed] [Google Scholar]
  20. TOMCHICK R., MANDEL H. G. BIOCHEMICAL EFFECTS OF ETHIDIUM BROMIDE IN MICRO-ORGANISMS. J Gen Microbiol. 1964 Aug;36:225–236. doi: 10.1099/00221287-36-2-225. [DOI] [PubMed] [Google Scholar]
  21. Van Alstyne D., Simon M. I. Division mutants of Bacillus subtilis: isolation and PBS1 transduction of division-specific markers. J Bacteriol. 1971 Dec;108(3):1366–1379. doi: 10.1128/jb.108.3.1366-1379.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Waring M. J. Structural requirements for the binding of ethidium to nucleic acids. Biochim Biophys Acta. 1966 Feb 21;114(2):234–244. doi: 10.1016/0005-2787(66)90305-4. [DOI] [PubMed] [Google Scholar]
  23. Young F. E., Smith C., Reilly B. E. Chromosomal location of genes regulating resistance to bacteriophage in Bacillus subtilis. J Bacteriol. 1969 Jun;98(3):1087–1097. doi: 10.1128/jb.98.3.1087-1097.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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