Abstract
Lipopolysaccharide prepared from cells of Yersinia (Pasteurella) pseudotuberculosis of serogroups I, II, III, IV, and V is known to contain the 3,6-dideoxyhexose (DDH) paratose, abequose, paratose, tyvelose, and ascarylose in its respective O-specific side chains. Lipopolysaccharides or lipid-free polysaccharides of all of the 10 known serogroups and subgroups were subjected to methylation analysis and determined as alditol acetates by gas-liquid chromatography and mass spectrometry. The results indicated that the O-specific side chains of nine serotypes are composed of oligosaccharide repeating units in the form of four alternative general structures in which a terminal DDH may vary. These structures are DDH [Formula: see text] 6-deoxy-d-manno-heptose [Formula: see text] d-galactose (serogroups IA, IIA, and IVB), DDH [Formula: see text] d-mannose [Formula: see text] l-fucose (serogroups IB and IIB), and two configurations similar to the latter except that the 4-position of l-fucose was either linked to the d-mannose residue (serogroups VA and VB) or to the DDH residue (serogroups III and IVA). In contrast, O-groups in lipopolysaccharide of the newly discovered serogroup VI contained the DDH colitose and 2-acetamido-2-deoxy-d-galactose. Accordingly, all five known types of DDH have now been detected in lipopolysaccharides of Y. pseudotuberculosis. The sugar 6-deoxy-d-manno-heptose, present in O-specific side chains of serogroups IA, IIA, and IVB, has not yet been reported to occur elsewhere in nature.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Borén H. B., Eklind K., Garegg P. J., Lindberg B., Pilotti A. Synthesis of 6-deoxy-D-manno-heptose. Acta Chem Scand. 1972;26(10):4143–4146. doi: 10.3891/acta.chem.scand.26-4143. [DOI] [PubMed] [Google Scholar]
- DAVIES D. A. Dideoxysugars of Pasteurella pseudotuberculosis-specific polysaccharides, and the occurrence of ascarylose. Nature. 1961 Jul 1;191:43–44. doi: 10.1038/191043a0. [DOI] [PubMed] [Google Scholar]
- DAVIES D. A. The smooth and rough somatic antigens of Pasteurella pseudotuberculosis. J Gen Microbiol. 1958 Feb;18(1):118–128. doi: 10.1099/00221287-18-1-118. [DOI] [PubMed] [Google Scholar]
- HAKOMORI S. A RAPID PERMETHYLATION OF GLYCOLIPID, AND POLYSACCHARIDE CATALYZED BY METHYLSULFINYL CARBANION IN DIMETHYL SULFOXIDE. J Biochem. 1964 Feb;55:205–208. [PubMed] [Google Scholar]
- HIGUCHI K., CARLIN C. E. Studies on the nutrition and physiology of Pasteurella pestis. II. A defined medium for the growth of Pasteurella pestis. J Bacteriol. 1958 Apr;75(4):409–413. doi: 10.1128/jb.75.4.409-413.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hellerqvist C. G., Lindberg B., Samuelsson K., Brubaker R. R. Structural studies on the O-specific side-chains of the cell-wall lipopolysaccharide from Pasteurella pseudo-tuberculosis Group II A. Acta Chem Scand. 1972;26(4):1389–1393. doi: 10.3891/acta.chem.scand.26-1389. [DOI] [PubMed] [Google Scholar]
- Hellerqvist C. G., Lindberg B., Samuelsson K., Brubaker R. R. Structural studies on the O-specific side-chains of the cell-wall lipopolysaccharide from Pasteurella pseudo-tuberculosis Group II B. Acta Chem Scand. 1972;26(4):1394–1398. doi: 10.3891/acta.chem.scand.26-1394. [DOI] [PubMed] [Google Scholar]
- KNAPP W. [On further antigen relations between Pasteurella pseudotuberculosis and the Salmonella group]. Z Hyg Infektionskr. 1960;146:315–330. [PubMed] [Google Scholar]
- LUDERITZ O., STAUB A. M., STIRM S., WESTPHAL O. Colitose (3-Desoxy-L-fucose), ein neuer Zuckerbaustein mit immunspezifischer Funktion in Endotoxinen (Lipopolysacchariden) einiger gram-negativer Bakterien. Biochem Z. 1958;330(3):193–197. [PubMed] [Google Scholar]