Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1974 May;118(2):654–662. doi: 10.1128/jb.118.2.654-662.1974

Detachment and Chemical Characterization of the Regularly Arranged Subunits from the Surface of an Acinetobacter

Margaret J Thornley a,1, Kareen J I Thorne a, Audrey M Glauert a
PMCID: PMC246801  PMID: 4208137

Abstract

Acinetobacter sp. strain MJT/F5/199A carries an array of tetragonally arranged subunits on its outer surface. The subunits can be detached from isolated cell walls by incubation with 1 M urea or by washing with water after treatment with 10 mM ethylenediaminetetraacetic acid or ethyleneglycol-bis(β-aminoethylether)N,N′-tetraacetic acid. After removal of the urea, they reaggregate into the same ordered array at air-water interfaces in the presence of MgCl2. The detached subunits were characterized as an acidic protein of molecular weight 65,000. They represent one-fifth of the total cell wall protein.

Full text

PDF
656

Images in this article

657Image
on p.657
658Image
on p.658
659Image
on p.659
660Image
on p.660
661Image
on p.661

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews P. The gel-filtration behaviour of proteins related to their molecular weights over a wide range. Biochem J. 1965 Sep;96(3):595–606. doi: 10.1042/bj0960595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Buckmire F. L., Murray R. G. Studies on the cell wall of Spirillum serpens. 1. Isolation and partial purification of the outermost cell wall layer. Can J Microbiol. 1970 Oct;16(10):1011–1022. doi: 10.1139/m70-171. [DOI] [PubMed] [Google Scholar]
  3. Buckmire F. L., Murray R. G. Studies on the cell wall of Spirillum serpens. II. Chemical characterization of the outer structured layer. Can J Microbiol. 1973 Jan;19(1):59–66. doi: 10.1139/m73-009. [DOI] [PubMed] [Google Scholar]
  4. Howard L., Tipper D. J. A polypeptide bacteriophage receptor: modified cell wall protein subunits in bacteriophage-resistant mutants of Bacillus sphaericus strain P-1. J Bacteriol. 1973 Mar;113(3):1491–1504. doi: 10.1128/jb.113.3.1491-1504.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Inouye M., Yee M. L. Homogeneity of envelope proteins of Escherichia coli separated by gel electrophoresis in sodium dodecyl sulfate. J Bacteriol. 1973 Jan;113(1):304–312. doi: 10.1128/jb.113.1.304-312.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Thorne K. J., Thornley M. J., Glauert A. M. Chemical analysis of the outer membrane and other layers of the cell envelope of Acinetobacter sp. J Bacteriol. 1973 Oct;116(1):410–417. doi: 10.1128/jb.116.1.410-417.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Thornley M. J., Glauert A. M., Sleytr U. B. Isolation of outer membranes with an ordered array of surface subunits from Acinetobacter. J Bacteriol. 1973 Jun;114(3):1294–1308. doi: 10.1128/jb.114.3.1294-1308.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
  10. Zacharius R. M., Zell T. E., Morrison J. H., Woodlock J. J. Glycoprotein staining following electrophoresis on acrylamide gels. Anal Biochem. 1969 Jul;30(1):148–152. doi: 10.1016/0003-2697(69)90383-2. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES