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. 1974 May;118(2):654–662. doi: 10.1128/jb.118.2.654-662.1974

Detachment and Chemical Characterization of the Regularly Arranged Subunits from the Surface of an Acinetobacter

Margaret J Thornley a,1, Kareen J I Thorne a, Audrey M Glauert a
PMCID: PMC246801  PMID: 4208137

Abstract

Acinetobacter sp. strain MJT/F5/199A carries an array of tetragonally arranged subunits on its outer surface. The subunits can be detached from isolated cell walls by incubation with 1 M urea or by washing with water after treatment with 10 mM ethylenediaminetetraacetic acid or ethyleneglycol-bis(β-aminoethylether)N,N′-tetraacetic acid. After removal of the urea, they reaggregate into the same ordered array at air-water interfaces in the presence of MgCl2. The detached subunits were characterized as an acidic protein of molecular weight 65,000. They represent one-fifth of the total cell wall protein.

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Selected References

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