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. 1974 Jun;118(3):783–789. doi: 10.1128/jb.118.3.783-789.1974

Mutants of Escherichia coli B/r Defective in Deoxyribonucleic Acid Initiation: dnaI, a New Gene for Replication

D Beyersmann a,1, W Messer a, M Schlicht a
PMCID: PMC246822  PMID: 4598003

Abstract

Mutagenized E. coli B/r cells were subjected to a procedure designed to select mutants temperature-sensitive for initiation of deoxyribonucleic acid (DNA) replication. Seventeen mutants exhibiting limited residual DNA synthesis at 42 C were obtained and the dna− sites were mapped genetically. Sixteen of the sites map near dnaA, dnaB, and dnaC. One mutant (dna-208) maps in a new location between the trp and his genes. We propose to call this mutant dnaI208. In complementation experiments dnaC+ and dnaI+ were dominant to dnaC− and dnaI− alleles, respectively. However, dnaA− was dominant to the wild-type allele dnaA+. All dnaA mutants and four out of six dnaC mutants could be suppressed by F factor integration. The pattern of suppression was specific for each mutant.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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