Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1974 Jun;118(3):810–814. doi: 10.1128/jb.118.3.810-814.1974

Direct Selection for P1-Sensitive Mutants of Enteric Bacteria

Richard B Goldberg 1, Robert A Bender 1, Stanley L Streicher 1
PMCID: PMC246826  PMID: 4598005

Abstract

A method has been developed to isolate mutants sensitive to coliphage P1 from bacterial genera normally not sensitive to this phage. P1clr100KM was used. This phage is heat inducible and confers kanamycin resistance when present as a prophage (in lysogens). P1-sensitive mutants of Klebsiella, Enterobacter, Citrobacter, and Erwinia have been found. This technique provides a well-known genetic system for the study of many bacterial genera that previously had either no such system or only a marginally useful means of genetic manipulation. It also extends the range of possible intergeneric hybrids that may be constructed and studied.

Full text

PDF
811

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARBER W., DUSSOIX D. Host specificity of DNA produced by Escherichia coli. I. Host controlled modification of bacteriophage lambda. J Mol Biol. 1962 Jul;5:18–36. doi: 10.1016/s0022-2836(62)80058-8. [DOI] [PubMed] [Google Scholar]
  2. Apirion D., Schlessinger D. Coresistance to neomycin and kanamycin by mutations in an Escherichia coli locus that affects ribosomes. J Bacteriol. 1968 Sep;96(3):768–776. doi: 10.1128/jb.96.3.768-776.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beck C. F., Ingraham J. L., Neuhard J. Location on the chromosome of Salmonella typhimurium of genes governing pyrimidine metabolism. II. Uridine kinase, cytosine deaminase and thymidine kinase. Mol Gen Genet. 1972;115(3):208–215. doi: 10.1007/BF00268884. [DOI] [PubMed] [Google Scholar]
  4. Brenchley J. E., Magasanik B. Klebsiella aerogenes strain carrying drug-resistance determinants and a lac plasmid. J Bacteriol. 1972 Oct;112(1):200–205. doi: 10.1128/jb.112.1.200-205.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gottesman M. M., Hicks M. L., Gellert M. Genetics and function of DNA ligase in Escherichia coli. J Mol Biol. 1973 Jul 15;77(4):531–547. doi: 10.1016/0022-2836(73)90221-0. [DOI] [PubMed] [Google Scholar]
  6. Ikeda H., Tomizawa J. I. Transducing fragments in generalized transduction by phage P1. I. Molecular origin of the fragments. J Mol Biol. 1965 Nov;14(1):85–109. doi: 10.1016/s0022-2836(65)80232-7. [DOI] [PubMed] [Google Scholar]
  7. LEDERBERG S. Suppression of the multiplication of heterologous bacteriophages in lysogenic bacteria. Virology. 1957 Jun;3(3):496–513. doi: 10.1016/0042-6822(57)90006-5. [DOI] [PubMed] [Google Scholar]
  8. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  9. MacPhee D. G., Sutherland I. W., Wilkinson J. F. Transduction in Klebsiella. Nature. 1969 Feb 1;221(5179):475–476. doi: 10.1038/221475a0. [DOI] [PubMed] [Google Scholar]
  10. Okada M., Watanabe T. Transduction with phage PI in Salmonella typhimurium. Nature. 1968 Apr 13;218(5137):185–187. doi: 10.1038/218185a0. [DOI] [PubMed] [Google Scholar]
  11. Prival M. J., Magasanik B. Resistance to catabolite repression of histidase and proline oxidase during nitrogen-limited growth of Klebsiella aerogenes. J Biol Chem. 1971 Oct 25;246(20):6288–6296. [PubMed] [Google Scholar]
  12. Rosner J. L. Formation, induction, and curing of bacteriophage P1 lysogens. Virology. 1972 Jun;48(3):679–689. doi: 10.1016/0042-6822(72)90152-3. [DOI] [PubMed] [Google Scholar]
  13. Sanderson K. E. Linkage map of Salmonella typhimurium, edition IV. Bacteriol Rev. 1972 Dec;36(4):558–586. doi: 10.1128/br.36.4.558-586.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Scott J. R. Genetic studies on bacteriophage P1. Virology. 1968 Dec;36(4):564–574. doi: 10.1016/0042-6822(68)90188-8. [DOI] [PubMed] [Google Scholar]
  15. Slocum H., Boyer H. W. Host specificity of Salmonella typhimurium deoxyribonucleic acid restriction and modification. J Bacteriol. 1973 Feb;113(2):724–726. doi: 10.1128/jb.113.2.724-726.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Smith H. O., Levine M. A phage P22 gene controlling integration of prophage. Virology. 1967 Feb;31(2):207–216. doi: 10.1016/0042-6822(67)90164-x. [DOI] [PubMed] [Google Scholar]
  17. Starr M. P., Chatterjee A. K. The genus Erwinia: enterobacteria pathogenic to plants and animals. Annu Rev Microbiol. 1972;26:389–426. doi: 10.1146/annurev.mi.26.100172.002133. [DOI] [PubMed] [Google Scholar]
  18. Stodolsky M. Bacteriophage P1 derivatives with bacterial genes: a heterozygote enrichment method for the selection of p1dpro lysogens. Virology. 1973 Jun;53(2):471–475. doi: 10.1016/0042-6822(73)90228-6. [DOI] [PubMed] [Google Scholar]
  19. Streicher S. L., Gurney E. G., Valentine R. C. The nitrogen fixation genes. Nature. 1972 Oct 27;239(5374):495–499. doi: 10.1038/239495a0. [DOI] [PubMed] [Google Scholar]
  20. Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wheelis M. L., Stanier R. Y. The genetic control of dissimilatory pathways in Pseudomonas putida. Genetics. 1970 Oct;66(2):245–266. doi: 10.1093/genetics/66.2.245. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES