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. 1971 Jul;107(1):239–244. doi: 10.1128/jb.107.1.239-244.1971

Nature and Origins of Phosphorus Compounds in Isolated Cell Walls of Staphylococcus aureus

David Mirelman a,1, D R D Shaw a, James T Park a
PMCID: PMC246910  PMID: 5563871

Abstract

Preparations of purified cell walls from Staphylococcus aureus were shown to contain small amounts of phospholipid and glycerol teichoic acid. Since these are components of the cell membrane, it is probable that the wall itself contains no lipid, but does retain fragments of membrane because of physical connections between wall and membrane. In walls of S. aureus strain 52A5, which completely lacks ribitol teichoic acid, the only phosphorylated compound identified as a genuine wall component was a phosphorylated derivative of murein that gave rise to muramic acid phosphate on acid hydrolysis. Muramic acid phosphate was also identified in hydrolysates of walls from S. aureus H and strain 52A2.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson J. S., Matsuhashi M., Haskin M. A., Strominger J. L. Biosythesis of the peptidoglycan of bacterial cell walls. II. Phospholipid carriers in the reaction sequence. J Biol Chem. 1967 Jul 10;242(13):3180–3190. [PubMed] [Google Scholar]
  2. Archibald A. R., Baddiley J. The teichoic acids. Adv Carbohydr Chem Biochem. 1966;21:323–375. doi: 10.1016/s0096-5332(08)60320-3. [DOI] [PubMed] [Google Scholar]
  3. CRITCHLEY P., ARCHIBALD A. R., BADDILEY J. The intracellular teichoic acid from Lactobacillus arabinosus 17-5. Biochem J. 1962 Dec;85:420–431. doi: 10.1042/bj0850420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dankert M., Wright A., Kelley W. S., Robbins P. W. Isolation, purification, and properties of the lipid-linked intermediates of O-antigen biosynthesis. Arch Biochem Biophys. 1966 Sep 26;116(1):425–435. doi: 10.1016/0003-9861(66)90049-x. [DOI] [PubMed] [Google Scholar]
  5. FEW A. V. The interaction of polymyxin E with bacterial and other lipids. Biochim Biophys Acta. 1955 Jan;16(1):137–145. doi: 10.1016/0006-3002(55)90191-8. [DOI] [PubMed] [Google Scholar]
  6. Forrester I. T., Wicken A. J. The chemical composition of the cell walls of some thermophilic bacilli. J Gen Microbiol. 1966 Jan;42(1):147–154. doi: 10.1099/00221287-42-1-147. [DOI] [PubMed] [Google Scholar]
  7. Hash J. H., Rothlauf M. V. The N,O-diacetylmuramidase of Chalaropsis species. I. Purification and crystallization. J Biol Chem. 1967 Dec 10;242(23):5586–5590. [PubMed] [Google Scholar]
  8. KELEMEN M. V., BADDILEY J. Structure of the intracellular glycerol teichoic acid from Lactobacillus casei A.T.C.C. 7469. Biochem J. 1961 Aug;80:246–254. doi: 10.1042/bj0800246. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Mirelman D., Beck B. D., Shaw D. R. The location of the D-alanyl ester in the ribitol teichoic acid of Staphylococcus aureus. Biochem Biophys Res Commun. 1970 May 22;39(4):712–717. doi: 10.1016/0006-291x(70)90263-9. [DOI] [PubMed] [Google Scholar]
  11. RAJBHANDARY U. L., BADDILEY J. The intracellular teichoic acid from Staphylococcus aureus H. Biochem J. 1963 May;87:429–435. doi: 10.1042/bj0870429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. RUNECKLES V. C., KROTKOV G. The separation of phosphate esters and other metabolites by ionophoresis and chromatography on paper. Arch Biochem Biophys. 1957 Aug;70(2):442–453. doi: 10.1016/0003-9861(57)90132-7. [DOI] [PubMed] [Google Scholar]
  13. Rogers H. J. Bacterial growth and the cell envelope. Bacteriol Rev. 1970 Jun;34(2):194–214. doi: 10.1128/br.34.2.194-214.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SALTON M. R. Studies of the bacterial cell wall. IV. The composition of the cell walls of some Gram-positive and Gram-negative bacteria. Biochim Biophys Acta. 1953 Apr;10(4):512–523. doi: 10.1016/0006-3002(53)90296-0. [DOI] [PubMed] [Google Scholar]
  15. Shaw D. R., Mirelman D., Chatterjee A. N., Park J. T. Ribitol teichoic acid synthesis in bacteriophage-resistant mutants of Staphylococcus aureus H. J Biol Chem. 1970 Oct 10;245(19):5101–5106. [PubMed] [Google Scholar]
  16. Shaw N., Baddiley J. The teichoic acid from the walls of Lactobacillus buchneri N.C.I.B. 8007. Biochem J. 1964 Nov;93(2):317–321. doi: 10.1042/bj0930317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. White D. C., Frerman F. E. Extraction, characterization, and cellular localization of the lipids of Staphylococcus aureus. J Bacteriol. 1967 Dec;94(6):1854–1867. doi: 10.1128/jb.94.6.1854-1867.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]

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