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. 1971 Oct;108(1):287–292. doi: 10.1128/jb.108.1.287-292.1971

Growth of Aerobacter aerogenes on d-Arabinose: Origin of the Enzyme Activities

Eugene J Oliver a,1, Robert P Mortlock a
PMCID: PMC247064  PMID: 5122807

Abstract

Mutants of Aerobacter aerogenes can be selected which are capable of utilizing d-arabinose as a sole source of carbon and energy for growth. Mutants can also be selected which are capable of using l-xylose. The mutational event permitting the utilization of d-arabinose results in the constitutive synthesis of certain enzymes of the l-fucose catabolic pathway. l-Fucose isomerase catalyzes the first reaction in the degradation of d-arabinose: the isomerization of d-arabinose to d-ribulose. No other type of mutation appears to be required to initiate growth on d-arabinose, and wild-type cells induced for the enzymes of the l-fucose catabolic pathway are capable of growth on d-arabinose. The first reaction in the catabolism of l-xylose, the isomerization of l-xylose to l-xylulose, also appears to be catalyzed by constitutively synthesized l-fucose isomerase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSON R. L., ALLISON D. P. PURIFICATION AND CHARACTERIZATION OF D-LYXOSE ISOMERASE. J Biol Chem. 1965 Jun;240:2367–2372. [PubMed] [Google Scholar]
  2. ANDERSON R. L., WOOD W. A. Pathway of L-xylose and L-lyxose degradation in Aerobacter aerogenes. J Biol Chem. 1962 Feb;237:296–303. [PubMed] [Google Scholar]
  3. ANDERSON R. L., WOOD W. A. Purification and properties of L-xylulokinase. J Biol Chem. 1962 Apr;237:1029–1033. [PubMed] [Google Scholar]
  4. Bisson T. M., Mortlock R. P. Regulation of pentitol metabolism by Aerobacter aerogenes. I. Coordinate control of ribitol dehydrogenase and D-ribulokinase activities. J Bacteriol. 1968 Mar;95(3):925–931. doi: 10.1128/jb.95.3.925-931.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bisson T. M., Oliver E. J., Mortlock R. P. Regulation of pentitol metabolism by aerobacter aerogenes. II. Induction of the ribitol pathway. J Bacteriol. 1968 Mar;95(3):932–936. doi: 10.1128/jb.95.3.932-936.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. COHEN S. Studies on D-ribulose and its enzymatic conversion to D-arabinose. J Biol Chem. 1953 Mar;201(1):71–84. [PubMed] [Google Scholar]
  7. Camyre K. P., Mortlock R. P. Growth of Aerobacter aerogenes on D-arabinose and L-xylose. J Bacteriol. 1965 Oct;90(4):1157–1158. doi: 10.1128/jb.90.4.1157-1158.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DISCHE Z., BORENFREUND E. A new spectrophotometric method for the detection and determination of keto sugars and trioses. J Biol Chem. 1951 Oct;192(2):583–587. [PubMed] [Google Scholar]
  9. Englesberg E., Irr J., Power J., Lee N. Positive control of enzyme synthesis by gene C in the L-arabinose system. J Bacteriol. 1965 Oct;90(4):946–957. doi: 10.1128/jb.90.4.946-957.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GHALAMBOR M. A., HEATH E. C. The metabolism of L-fucose. II. The enzymatic cleavage of L-fuculose 1-phosphate. J Biol Chem. 1962 Aug;237:2427–2433. [PubMed] [Google Scholar]
  11. GREEN M., COHEN S. S. Enzymatic conversion of L-fucose to L-fuculose. J Biol Chem. 1956 Apr;219(2):557–568. [PubMed] [Google Scholar]
  12. HEATH E. C., GHALAMBOR M. A. The metabolism of L-fucose. I. The purification and properties of L-fuculose kinase. J Biol Chem. 1962 Aug;237:2423–2426. [PubMed] [Google Scholar]
  13. MORTLOCK R. P., FOSSITT D. D., PETERING D. H., WOOD W. A. METABOLISM OF PENTOSES AND PENTITOLS BY AEROBACTER AEROGENES. 3. PHYSICAL AND IMMUNOLOGICAL PROPERTIES OF PENITOL DEHYDROGENASES AND PENTULOKINASES. J Bacteriol. 1965 Jan;89:129–135. doi: 10.1128/jb.89.1.129-135.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. NEISH A. C., SIMPSON F. J. The anaerobic dissimilation of d-glucose-1-C14, d-arabinose-1-C14, and 1-arabinose-1-C14 by Aerobacter aerogenes. Can J Biochem Physiol. 1954 May;32(3):147–153. [PubMed] [Google Scholar]
  15. Oliver E. J., Bisson T. M., LeBlanc D. J., Mortlock R. P. D-Ribulose production by a mutant of Aerobacter aerogens. Anal Biochem. 1969 Feb;27(2):300–305. doi: 10.1016/0003-2697(69)90036-0. [DOI] [PubMed] [Google Scholar]
  16. Wood W. A. Carbohydrate metabolism. Annu Rev Biochem. 1966;35:521–558. doi: 10.1146/annurev.bi.35.070166.002513. [DOI] [PubMed] [Google Scholar]

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