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. 1971 Oct;108(1):375–385. doi: 10.1128/jb.108.1.375-385.1971

On the Process of Cellular Division in Escherichia coli VI. Use of a Methocel-Autoradiographic Method for the Study of Cellular Division in Escherichia coli

E C C Lin 1, Y Hirota 1, F Jacob 1
PMCID: PMC247076  PMID: 4107811

Abstract

A technique allowing chain formation by Escherichia coli for the analysis of the segregation of certain cellular components by autoradiographic methods has been developed. Deoxyribonucleic acid labeled with 3H-thymidine does not appear to segregate preferentially with one or another of the bacteria. Membrane lipids labeled with either 3H-oleate or 3H-glycerol are evenly distributed among all progeny cells. A similar pattern of distribution of cell wall material is observed when 3H-diaminopimelate is used as the marker.

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Selected References

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  1. Ames G. F. Lipids of Salmonella typhimurium and Escherichia coli: structure and metabolism. J Bacteriol. 1968 Mar;95(3):833–843. doi: 10.1128/jb.95.3.833-843.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barbour S. D., Nagaishi H., Templin A., Clark A. J. Biochemical and genetic studies of recombination proficiency in Escherichia coli. II. Rec+ revertants caused by indirect suppression of rec- mutations. Proc Natl Acad Sci U S A. 1970 Sep;67(1):128–135. doi: 10.1073/pnas.67.1.128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bazill G. W. Lethal unbalanced growth in bacteria. Nature. 1967 Oct 28;216(5113):346–349. doi: 10.1038/216346a0. [DOI] [PubMed] [Google Scholar]
  4. Beacham I. R., Eisenstark A., Barth P. T., Pritchard R. H. Deoxynucleoside-sensitive mutants of Salmonella typhimurium. Mol Gen Genet. 1968;102(2):112–127. doi: 10.1007/BF01789138. [DOI] [PubMed] [Google Scholar]
  5. Briles E. B., Tomasz A. Radioautographic evidence for equatorial wall growth in a gram-positive bacterium. Segregation of choline-3H-labeled teichoic acid. J Cell Biol. 1970 Dec;47(3):786–790. doi: 10.1083/jcb.47.3.786. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. CARO L. G., VAN TUBERGEN R. P., KOLB J. A. High-resolution autoradiography. I. Methods. J Cell Biol. 1962 Nov;15:173–188. doi: 10.1083/jcb.15.2.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. COHN M., HORIBATA K. Analysis of the differentiation and of the heterogeneity within a population of Escherichia coli undergoing induced beta-galactosidase synthesis. J Bacteriol. 1959 Nov;78:613–623. doi: 10.1128/jb.78.5.613-623.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. COLE R. M. CELL WALL REPLICATION IN SALMONELLA TYPHOSA. Science. 1964 Feb 21;143(3608):820–822. doi: 10.1126/science.143.3608.820. [DOI] [PubMed] [Google Scholar]
  9. Caro L. G., Schnös M. Tritium and Phosphorus-32 in High-Resolution Autoradiography. Science. 1965 Jul 2;149(3679):60–62. doi: 10.1126/science.149.3679.60. [DOI] [PubMed] [Google Scholar]
  10. Cole R. M. Symposium on the fine structure and replication of bacteria and their parts. 3. Bacterial cell-wall replication followed by immunofluorescence. Bacteriol Rev. 1965 Sep;29(3):326–344. doi: 10.1128/br.29.3.326-344.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Cozzarelli N. R., Freedberg W. B., Lin E. C. Genetic control of L-alpha-glycerophosphate system in Escherichia coli. J Mol Biol. 1968 Feb 14;31(3):371–387. doi: 10.1016/0022-2836(68)90415-4. [DOI] [PubMed] [Google Scholar]
  12. Cronan J. E., Jr Phospholipid alterations during growth of Escherichia coli. J Bacteriol. 1968 Jun;95(6):2054–2061. doi: 10.1128/jb.95.6.2054-2061.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Donachie W. D., Begg K. J. Growth of the bacterial cell. Nature. 1970 Sep 19;227(5264):1220–1224. doi: 10.1038/2271220a0. [DOI] [PubMed] [Google Scholar]
  14. Emmerson P. T. Recombination deficient mutants of Escherichia coli K12 that map between thy A and argA. Genetics. 1968 Sep;60(1):19–30. doi: 10.1093/genetics/60.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. FORRO F., Jr, WERTHEIMER S. A. The organization and replication of deoxyribonucleic acid in thymine-deficient strains of Escherichia coli. Biochim Biophys Acta. 1960 May 6;40:9–21. doi: 10.1016/0006-3002(60)91310-x. [DOI] [PubMed] [Google Scholar]
  16. Fielding P., Fox C. F. Evidence for stable attachment of DNA to membrane at the replication origin of Escherichia coli. Biochem Biophys Res Commun. 1970 Oct 9;41(1):157–162. doi: 10.1016/0006-291x(70)90482-1. [DOI] [PubMed] [Google Scholar]
  17. HAYASHI S., KOCH J. P., LIN E. C. ACTIVE TRANSPORT OF L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI. J Biol Chem. 1964 Sep;239:3098–3105. [PubMed] [Google Scholar]
  18. Haefner K. Spontaneous lethal sectoring, a further feature of Escherichia coli strains deficient in the function of rec and uvr genes. J Bacteriol. 1968 Sep;96(3):652–659. doi: 10.1128/jb.96.3.652-659.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. KANFER J., KENNEDY E. P. METABOLISM AND FUNCTION OF BACTERIAL LIPIDS. I. METABOLISM OF PHOSPHOLIPIDS IN ESCHERICHIA COLI B. J Biol Chem. 1963 Sep;238:2919–2922. [PubMed] [Google Scholar]
  20. KOCH J. P., HAYASHI S., LIN E. C. THE CONTROL OF DISSIMILATION OF GLYCEROL AND L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI. J Biol Chem. 1964 Sep;239:3106–3108. [PubMed] [Google Scholar]
  21. Kistler W. S., Hirsch C. A., Cozzarelli N. R., Lin E. C. Second pyridine nucleotide-independent 1-alpha-glycerophosphate dehydrogenase in Escherichia coli K-12. J Bacteriol. 1969 Nov;100(2):1133–1135. doi: 10.1128/jb.100.2.1133-1135.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Lark K. G. Regulation of chromosome replication and segregation in bacteria. Bacteriol Rev. 1966 Mar;30(1):3–32. doi: 10.1128/br.30.1.3-32.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Lederberg J. Linear Inheritance in Transductional Clones. Genetics. 1956 Nov;41(6):845–871. doi: 10.1093/genetics/41.6.845. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. MONOD J., COHEN-BAZIRE G., COHN M. Sur la biosynthèse de la beta-galactosidase (lactase) chez Escherichia coli; la spécificité de l'induction. Biochim Biophys Acta. 1951 Nov;7(4):585–599. doi: 10.1016/0006-3002(51)90072-8. [DOI] [PubMed] [Google Scholar]
  25. Mindich L. Membrane synthesis in Bacillus subtilis. I. Isolation and properties of strains bearing mutations in glycerol metabolism. J Mol Biol. 1970 Apr 28;49(2):415–432. doi: 10.1016/0022-2836(70)90254-8. [DOI] [PubMed] [Google Scholar]
  26. Mindich L. Membrane synthesis in Bacillus subtilis. II. Integration of membrane proteins in the absence of lipid synthesis. J Mol Biol. 1970 Apr 28;49(2):433–439. doi: 10.1016/0022-2836(70)90255-x. [DOI] [PubMed] [Google Scholar]
  27. Morrison D. C., Morowitz H. J. Studies on membrane synthesis in Bacillus megaterium KM. J Mol Biol. 1970 Apr 28;49(2):441–459. doi: 10.1016/0022-2836(70)90256-1. [DOI] [PubMed] [Google Scholar]
  28. QUADLING C., STOCKER B. A. An environmentally-induced transition from the flagellated to the non-flagellated state in Salmonella typhimurium: the fate of parental flagella at cell division. J Gen Microbiol. 1962 Jun;28:257–270. doi: 10.1099/00221287-28-2-257. [DOI] [PubMed] [Google Scholar]
  29. Rogers H. J. Bacterial growth and the cell envelope. Bacteriol Rev. 1970 Jun;34(2):194–214. doi: 10.1128/br.34.2.194-214.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Ryter A. Association of the nucleus and the membrane of bacteria: a morphological study. Bacteriol Rev. 1968 Mar;32(1):39–54. doi: 10.1128/br.32.1.39-54.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Ryter A., Hirota Y., Jacob F. DNA-membrane complex and nuclear segregation in bacteria. Cold Spring Harb Symp Quant Biol. 1968;33:669–676. doi: 10.1101/sqb.1968.033.01.076. [DOI] [PubMed] [Google Scholar]
  32. Ryter A., Jacob F. Ségrégation des noyaux pendant la croissance et la germination de B. subtilis. C R Acad Sci Hebd Seances Acad Sci D. 1967 May 3;264(18):2254–2256. [PubMed] [Google Scholar]
  33. SCHAECHTER M., MAALOE O., KJELDGAARD N. O. Dependency on medium and temperature of cell size and chemical composition during balanced grown of Salmonella typhimurium. J Gen Microbiol. 1958 Dec;19(3):592–606. doi: 10.1099/00221287-19-3-592. [DOI] [PubMed] [Google Scholar]
  34. STACEY K. A., SIMSON E. IMPROVED METHOD FOR THE ISOLATION OF THYMINE-REQUIRING MUTANTS OF ESCHERICHIA COLI. J Bacteriol. 1965 Aug;90:554–555. doi: 10.1128/jb.90.2.554-555.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Schwarz U., Asmus A., Frank H. Autolytic enzymes and cell division of Escherichia coli. J Mol Biol. 1969 May 14;41(3):419–429. doi: 10.1016/0022-2836(69)90285-x. [DOI] [PubMed] [Google Scholar]
  36. Smith D. W., Hanawalt P. C. Properties of the growing point region in the bacterial chromosome. Biochim Biophys Acta. 1967 Dec 19;149(2):519–531. doi: 10.1016/0005-2787(67)90180-3. [DOI] [PubMed] [Google Scholar]
  37. Sueoka N., Quinn W. G. Membrane attachment of the chromosome replication origin in Bacillus subtilis. Cold Spring Harb Symp Quant Biol. 1968;33:695–705. doi: 10.1101/sqb.1968.033.01.078. [DOI] [PubMed] [Google Scholar]
  38. VAN TUBERGEN R. P., SETLOW R. B. Quantitative radioautographic studies on exponentially growing cultures of Escherichia coli. The distribution of parental DNA, RNA, protein, and cell wall among progeny cells. Biophys J. 1961 Sep;1:589–625. doi: 10.1016/s0006-3495(61)86911-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Willetts N. S., Mount D. W. Genetic analysis of recombination-deficient mutants of Escherichia coli K-12 carrying rec mutations cotransducible with thyA. J Bacteriol. 1969 Nov;100(2):923–934. doi: 10.1128/jb.100.2.923-934.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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