Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1971 Nov;108(2):690–694. doi: 10.1128/jb.108.2.690-694.1971

Biochemical Characterization of the ctr Mutants of Escherichia coli1

Helvise G Morse a, William K Penberthy a, M L Morse a
PMCID: PMC247127  PMID: 4942759

Abstract

A test procedure based on complementation in mixed extracts is described for the assay of heat-stable protein and enzyme I of the phosphoenolpyruvate-dependent phosphotransferase system. The test was used to assay a collection of pleiotropic carbohydrate mutants of Escherichia coli (ctr mutants) and revertants of these mutants. All mutants were found to lack enzyme I of the phosphoenolpyruvate-dependent transferase system. Revertants of these mutants to complete wild phenotype regained enzyme I-forming ability. Reversion to partial wild type was not accompanied by restoration of enzyme I-forming ability.

Full text

PDF
693

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dahl R., Wang R. J., Morse M. L. Effect of pleiotropic carbohydrate mutations (ctr) on tryptophan catabolism. J Bacteriol. 1971 Aug;107(2):513–518. doi: 10.1128/jb.107.2.513-518.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fox C. F., Wilson G. The role of a phosphoenolpyruvate-dependent kinase system in beta-glucoside catabolism in Escherichia coli. Proc Natl Acad Sci U S A. 1968 Mar;59(3):988–995. doi: 10.1073/pnas.59.3.988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  4. MORSE M. L., ALIRE M. L. An agar medium indicating acid production. J Bacteriol. 1958 Sep;76(3):270–271. doi: 10.1128/jb.76.3.270-271.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Saier M. H., Jr, Simoni R. D., Roseman S. The physiological behavior of enzyme I and heat-stable protein mutants of a bacterial phosphotransferase system. J Biol Chem. 1970 Nov 10;245(21):5870–5873. [PubMed] [Google Scholar]
  6. Tanaka S., Fraenkel D. G., Lin E. C. The enzymatic lesion of strain MM-6, a pleiotropic carbohydrate-negative mutant of Escherichia coli. Biochem Biophys Res Commun. 1967 Apr 7;27(1):63–67. doi: 10.1016/s0006-291x(67)80040-8. [DOI] [PubMed] [Google Scholar]
  7. Taylor A. L. Current linkage map of Escherichia coli. Bacteriol Rev. 1970 Jun;34(2):155–175. doi: 10.1128/br.34.2.155-175.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Wang R. J., Morse H. G., Morse M. L. Carbohydrate Accumulation and Metabolism in Escherichia coli: Characteristics of the Reversions of ctr Mutations. J Bacteriol. 1970 Dec;104(3):1318–1324. doi: 10.1128/jb.104.3.1318-1324.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Wang R. J., Morse H. G., Morse M. L. Carbohydrate accumulation and metabolism in Escherichia coli: the close linkage and chromosomal location of ctr mutations. J Bacteriol. 1969 May;98(2):605–610. doi: 10.1128/jb.98.2.605-610.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Wang R. J., Morse M. L. Carbohydrate accumulation and metabolism in Escherichia coli. I. Description of pleiotropic mutants. J Mol Biol. 1968 Feb 28;32(1):59–66. doi: 10.1016/0022-2836(68)90145-9. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES