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. 1971 Dec;108(3):1001–1007. doi: 10.1128/jb.108.3.1001-1007.1971

Role of Vitamin B6 Biosynthetic Rate in the Study of Vitamin B6 Synthesis in Escherichia coli

Walter B Dempsey 1
PMCID: PMC247181  PMID: 4945178

Abstract

Nutritional auxotrophs of Escherichia coli synthesize vitamin B6 compounds at a rate of 1 × 10−10 to 2 × 10−10 moles per hr per mg (dry weight) of cells when they are suspended in minimal medium lacking their required nutrients. A few auxotrophs have been found to stop or reduce vitamin B6 synthesis during such an experiment. These include thiamineless, citrate synthaseless, and pyridoxineless mutants as well as mutants which require four carbon compounds for growth. Glycolaldehyde was found to restore vitamin B6 synthesis in the last named of these mutants without restoring normal growth. A class of pyridoxineless mutants which responded with normal growth to 0.4 mm glycolaldehyde or 0.15 × 10−3 mm pyridoxol was also found. The results suggest that a thiamine pyrophosphate-requiring step as well as glycolaldehyde may be involved in pyridoxal phosphate biosynthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ASHWORTH J. M., KORNBERG H. L., NOTHMANN D. L. LOCATION OF THE STRUCTURAL GENE FOR CITRATE SYNTHASE ON THE CHROMOSOME OF ESCHERICHIA COLI K12. J Mol Biol. 1965 Mar;11:654–657. doi: 10.1016/s0022-2836(65)80021-3. [DOI] [PubMed] [Google Scholar]
  2. Bukhari A. I., Taylor A. L. Mutants of Escherichia coli with a growth requirement for either lysine or pyridoxine. J Bacteriol. 1971 Mar;105(3):988–998. doi: 10.1128/jb.105.3.988-998.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DEMPSEY W. B. CONTROL OF PYRIDOXINE BIOSYNTHESIS IN ESCHERICHIA COLI. J Bacteriol. 1965 Aug;90:431–437. doi: 10.1128/jb.90.2.431-437.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dempsey W. B. Characterization of pyridoxine auxotrophs of Escherichia coli: P1 transduction. J Bacteriol. 1969 Mar;97(3):1403–1410. doi: 10.1128/jb.97.3.1403-1410.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dempsey W. B. Characterization of pyridoxine auxotrophs of Escherichia coli: chromosomal position of linkage group I. J Bacteriol. 1969 Oct;100(1):295–300. doi: 10.1128/jb.100.1.295-300.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dempsey W. B., Ito H. Characterization of pyridoxine auxotrophs of Escherichia coli: serine and pdxF mutants. J Bacteriol. 1970 Nov;104(2):658–667. doi: 10.1128/jb.104.2.658-667.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dempsey W. B. One-carbon metabolism and pyridoxal 5'-phosphate biosynthesis. Biochim Biophys Acta. 1970 Dec 29;222(3):686–687. doi: 10.1016/0304-4165(70)90201-1. [DOI] [PubMed] [Google Scholar]
  8. Dempsey W. B., Pachler P. F. Isolation and characterization of pyridoxine auxotrophs of Escherichia coli. J Bacteriol. 1966 Feb;91(2):642–645. doi: 10.1128/jb.91.2.642-645.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hill R. E., Gupta R. N., Rowell F. J., Spenser I. D. Biosynthesis of pyridoxine. J Am Chem Soc. 1971 Jan 27;93(2):518–520. doi: 10.1021/ja00731a038. [DOI] [PubMed] [Google Scholar]
  10. MORRIS J. G., HUGHES D. T., MULDER C. Observations on the assay of vitamin B6 with Saccharomyces carlsbergensis 4228. J Gen Microbiol. 1959 Jun;20(3):566–575. doi: 10.1099/00221287-20-3-566. [DOI] [PubMed] [Google Scholar]
  11. MORRIS J. G. The synthesis of vitamin B6 by some mutant strains of Escherichia coli. J Gen Microbiol. 1959 Jun;20(3):597–604. doi: 10.1099/00221287-20-3-597. [DOI] [PubMed] [Google Scholar]
  12. MORRIS J. G., WOODS D. D. Inter-relationships of serine, glycine and vitamin B6 in the growth of mutants of Escherichia coli. J Gen Microbiol. 1959 Jun;20(3):576–596. doi: 10.1099/00221287-20-3-576. [DOI] [PubMed] [Google Scholar]
  13. Pittard J., Wallace B. J. Distribution and function of genes concerned with aromatic biosynthesis in Escherichia coli. J Bacteriol. 1966 Apr;91(4):1494–1508. doi: 10.1128/jb.91.4.1494-1508.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Suzue R., Haruna Y. Biosynthesis of vitamin B6. II. Localization of 14C-carbons in vitamin B6. J Vitaminol (Kyoto) 1970 Sep;16(3):161–163. doi: 10.5925/jnsv1954.16.161. [DOI] [PubMed] [Google Scholar]
  15. Taylor A. L. Current linkage map of Escherichia coli. Bacteriol Rev. 1970 Jun;34(2):155–175. doi: 10.1128/br.34.2.155-175.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. WILSON A. C., PARDEE A. B. Regulation of flavin synthesis by Escherichia coli. J Gen Microbiol. 1962 Jun;28:283–303. doi: 10.1099/00221287-28-2-283. [DOI] [PubMed] [Google Scholar]

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