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. 1971 Dec;108(3):1270–1276. doi: 10.1128/jb.108.3.1270-1276.1971

Genetics of the Mandelate Pathway in Pseudomonas aeruginosa1

S L Rosenberg a,2, G D Hegeman a
PMCID: PMC247214  PMID: 5003177

Abstract

The linkage of genes governing synthesis of enzymes functional in the conversion of l(+)-mandelate to benzoate in Pseudomonas aeruginosa was studied. These genes were found to be linked in a fashion consistent with regulatory mechanisms known to govern synthesis of this inducible pathway. Surprisingly, linkage was also observed between the mandelate genes and those governing the synthesis of functionally related enzymes that participate in the catabolism of anthranilate and benzoate. These latter genes are grouped in at least three regulatory units. Some theoretical explanations for high intensity of linkage among functionally related genes whose expression is not induced by a common metabolite are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURTON J. F., CAIN B. F. Antileukaemic activity of polyporic acid. Nature. 1959 Oct 24;184(Suppl 17):1326–1327. doi: 10.1038/1841326a0. [DOI] [PubMed] [Google Scholar]
  2. CLOWES R. C. Investigation of the genetics of cysteineless mutants of Salmonella typhimurium by transduction. J Gen Microbiol. 1958 Feb;18(1):154–172. doi: 10.1099/00221287-18-1-154. [DOI] [PubMed] [Google Scholar]
  3. Chakrabarty A. M., Gunsalus C. F., Gunsalus I. C. Transduction and the clustering of genes in fluorescent Pseudomonads. Proc Natl Acad Sci U S A. 1968 May;60(1):168–175. doi: 10.1073/pnas.60.1.168. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chakrabarty A. M., Gunsalus I. C. Autonomous replication of a defective transducing phage in Pseudomonas putida. Virology. 1969 May;38(1):92–104. doi: 10.1016/0042-6822(69)90131-7. [DOI] [PubMed] [Google Scholar]
  5. FARGIE B., HOLLOWAY B. W. ABSENCE OF CLUSTERING OF FUNCTIONALLY RELATED GENES IN PSEUDOMONAS AERUGINOSA. Genet Res. 1965 Jul;6:284–299. doi: 10.1017/s0016672300004158. [DOI] [PubMed] [Google Scholar]
  6. GUNSALUS C. F., STANIER R. Y., GUNSALUS I. C. The enzymatic conversion of mandelic acid to benzoic acid. III. Fractionation and properties of the soluble enzymes. J Bacteriol. 1953 Nov;66(5):548–553. doi: 10.1128/jb.66.5.548-553.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GUNSALUS I. C., GUNSALUS C. F., STANIER R. Y. The enzymatic conversion of mandelic acid to benzoic acid. I. Gross fractionation of the system into soluble and particulate components. J Bacteriol. 1953 Nov;66(5):538–542. doi: 10.1128/jb.66.5.538-542.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hegeman G. D., Rosenberg S. L. The evolution of bacterial enzyme systems. Annu Rev Microbiol. 1970;24:429–462. doi: 10.1146/annurev.mi.24.100170.002241. [DOI] [PubMed] [Google Scholar]
  9. Hegeman G. D. Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. 3. Isolation and properties of constitutive mutants. J Bacteriol. 1966 Mar;91(3):1161–1167. doi: 10.1128/jb.91.3.1161-1167.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hegeman G. D. Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. I. Synthesis of enzymes by the wild type. J Bacteriol. 1966 Mar;91(3):1140–1154. doi: 10.1128/jb.91.3.1140-1154.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hegeman G. D. Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. II. Isolation and properties of blocked mutants. J Bacteriol. 1966 Mar;91(3):1155–1160. doi: 10.1128/jb.91.3.1155-1160.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Holloway B. W. Genetics of Pseudomonas. Bacteriol Rev. 1969 Sep;33(3):419–443. doi: 10.1128/br.33.3.419-443.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hopwood D. A. Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriol Rev. 1967 Dec;31(4):373–403. doi: 10.1128/br.31.4.373-403.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. JACOB F., MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318–356. doi: 10.1016/s0022-2836(61)80072-7. [DOI] [PubMed] [Google Scholar]
  15. Kemp M. B., Hegeman G. D. Genetic control of the beta-ketoadipate pathway in Pseudomonas aeruginosa. J Bacteriol. 1968 Nov;96(5):1488–1499. doi: 10.1128/jb.96.5.1488-1499.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nei M. Modification of linkage intensity by natural selection. Genetics. 1967 Nov;57(3):625–641. doi: 10.1093/genetics/57.3.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ornston L. N., Stanier R. Y. The conversion of catechol and protocatechuate to beta-ketoadipate by Pseudomonas putida. J Biol Chem. 1966 Aug 25;241(16):3776–3786. [PubMed] [Google Scholar]
  18. Ornston L. N. The conversion of catechol and protocatechuate to beta-ketoadipate by Pseudomonas putida. IV. Regulation. J Biol Chem. 1966 Aug 25;241(16):3800–3810. [PubMed] [Google Scholar]
  19. Reiner A. M., Hegeman G. D. Metabolism of benzoic acid by bacteria. Accumulation of (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid by mutant strain of Alcaligenes eutrophus. Biochemistry. 1971 Jun 22;10(13):2530–2536. doi: 10.1021/bi00789a017. [DOI] [PubMed] [Google Scholar]
  20. Rosenberg S. L., Hegeman G. D. Clustering of functionally related genes in Pseudomonas aeruginosa. J Bacteriol. 1969 Jul;99(1):353–355. doi: 10.1128/jb.99.1.353-355.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rosenberg S. L. Regulation of the mandelate pathway in Pseudomonas aeruginosa. J Bacteriol. 1971 Dec;108(3):1257–1269. doi: 10.1128/jb.108.3.1257-1269.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. STANIER R. Y., GUNSALUS I. C., GUNSALUS C. F. The enzymatic conversion of mandelic acid to benzoic acid. II. Properties of the particulate fractions. J Bacteriol. 1953 Nov;66(5):543–547. doi: 10.1128/jb.66.5.543-547.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sanderson K. E. Current linkage map of Salmonella typhimurium. Bacteriol Rev. 1970 Jun;34(2):176–193. doi: 10.1128/br.34.2.176-193.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Stahl F. W., Murray N. E. The evolution of gene clusters and genetic circularity in microorganisms. Genetics. 1966 Mar;53(3):569–576. doi: 10.1093/genetics/53.3.569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Stanier R. Y., Wachter D., Gasser C., Wilson A. C. Comparative immunological studies of two Pseudomonas enzymes. J Bacteriol. 1970 May;102(2):351–362. doi: 10.1128/jb.102.2.351-362.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Taylor A. L. Current linkage map of Escherichia coli. Bacteriol Rev. 1970 Jun;34(2):155–175. doi: 10.1128/br.34.2.155-175.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. WILLSON C., PERRIN D., COHN M., JACOB F., MONOD J. NON-INDUCIBLE MUTANTS OF THE REGULATOR GENE IN THE "LACTOSE" SYSTEM OF ESCHERICHIA COLI. J Mol Biol. 1964 Apr;8:582–592. doi: 10.1016/s0022-2836(64)80013-9. [DOI] [PubMed] [Google Scholar]
  28. Wheelis M. L., Stanier R. Y. The genetic control of dissimilatory pathways in Pseudomonas putida. Genetics. 1970 Oct;66(2):245–266. doi: 10.1093/genetics/66.2.245. [DOI] [PMC free article] [PubMed] [Google Scholar]

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