Abstract
A new mutation in Escherichia coli, giving inability to grow on gluconic, glucuronic, or galacturonic acids, has been identified as complete deficiency of 2-keto-3-deoxygluconate 6-phosphate (KDGP) aldolase activity. The genetic map position of the locus, eda, is about 35 min. The inability to grow on the uronic acids was expected, because the aldolase is on the sole known pathway of their metabolism. However, inability to grow on gluconate was less expected, because the hexose monophosphate shunt might be used, as happens in mutants blocked in the previous step, edd, of the Entner-Doudoroff pathway. The likely explanation of gluconate negativity is inhibition by accumulated KDGP, because gluconate is inhibitory to growth on other substances, and one type of gluconate revertant is eda−, edd−. KDGP is probably the inducer of KDGP aldolase.
Full text
PDF![1277](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df8a/247215/435db255cdc9/jbacter00367-0343.png)
![1278](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df8a/247215/a9467ae2bfd3/jbacter00367-0344.png)
![1279](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df8a/247215/16ddf7d18624/jbacter00367-0345.png)
![1280](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df8a/247215/59131ec72ff8/jbacter00367-0346.png)
![1281](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df8a/247215/d48e332065bc/jbacter00367-0347.png)
![1282](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df8a/247215/acd7bbd19d5a/jbacter00367-0348.png)
![1283](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df8a/247215/4daee08d1acb/jbacter00367-0349.png)
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baudouy-Robert J., Didier-Fichet M. L., Jimeno-Abendano J., Novel G., Portalier R., Stoeber F. Modalités de l'induction des six premières enzymes dégradant les hexuronides et les hexuronates chez Escherichia coli K 12. C R Acad Sci Hebd Seances Acad Sci D. 1970 Jul 15;271(2):255–258. [PubMed] [Google Scholar]
- Böck A., Neidhardt F. C. Properties of a Mutant of Escherichia coli with a Temperature-sensitive Fructose-1,6-Diphosphate Aldolase. J Bacteriol. 1966 Aug;92(2):470–476. doi: 10.1128/jb.92.2.470-476.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COHEN S. S. Gluconokinase and the oxidative path of glucose-6-phosphate utilization. J Biol Chem. 1951 Apr;189(2):617–628. [PubMed] [Google Scholar]
- ENTNER N., DOUDOROFF M. Glucose and gluconic acid oxidation of Pseudomonas saccharophila. J Biol Chem. 1952 May;196(2):853–862. [PubMed] [Google Scholar]
- Eisenberg R. C., Dobrogosz W. J. Gluconate metabolism in Escherichia coli. J Bacteriol. 1967 Mar;93(3):941–949. doi: 10.1128/jb.93.3.941-949.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fraenkel D. G. Genetic mapping of mutations affecting phosphoglucose isomerase and fructose diphosphatase in Escherichia coli. J Bacteriol. 1967 May;93(5):1582–1587. doi: 10.1128/jb.93.5.1582-1587.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fraenkel D. G., Horecker B. L. Fructose-1, 6-diphosphatase and acid hexose phosphatase of Escherichia coli. J Bacteriol. 1965 Oct;90(4):837–842. doi: 10.1128/jb.90.4.837-842.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fraenkel D. G., Levisohn S. R. Glucose and gluconate metabolism in an Escherichia coli mutant lacking phosphoglucose isomerase. J Bacteriol. 1967 May;93(5):1571–1578. doi: 10.1128/jb.93.5.1571-1578.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fraenkel D. G. Selection of Escherichia coli mutants lacking glucose-6-phosphate dehydrogenase or gluconate-6-phosphate dehydrogenase. J Bacteriol. 1968 Apr;95(4):1267–1271. doi: 10.1128/jb.95.4.1267-1271.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fraenkel D. G. The accumulation of glucose 6-phosphate from glucose and its effect in an Escherichia coli mutant lacking phosphoglucose isomerase and glucose 6-phosphate dehydrogenase. J Biol Chem. 1968 Dec 25;243(24):6451–6457. [PubMed] [Google Scholar]
- KOVACHEVICH R., WOOD W. A. Carbohydrate metabolism by Pseudomonas fluorescens. III. Purification and properties of a 6-phosphogluconate dehydrase. J Biol Chem. 1955 Apr;213(2):745–756. [PubMed] [Google Scholar]
- LOVELESS A., HOWARTH S. Mutation of bacteria at high levels of survival by ethyl methane sulphonate. Nature. 1959 Dec 5;184:1780–1782. doi: 10.1038/1841780a0. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Novel G., Novel M., Didier-Fichet M. L., Stoeber F. Etude génétique de mutants du système de dégradation des hexuronides chez Escherichia coli K 12. C R Acad Sci Hebd Seances Acad Sci D. 1970 Jul 27;271(4):457–460. [PubMed] [Google Scholar]
- Peyru G., Fraenkel D. G. Genetic mapping of loci for glucose-6-phosphate dehydrogenase, gluconate-6-phosphate dehydrogenase, and gluconate-6-phosphate dehydrase in Escherichia coli. J Bacteriol. 1968 Apr;95(4):1272–1278. doi: 10.1128/jb.95.4.1272-1278.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pouysségur J., Stoeber F. Sur la biosynthèse induite des deux dernières enzymes de la séquence dégradative des hexuronates chez Escherichia coli K 12. C R Acad Sci Hebd Seances Acad Sci D. 1970 Jul 20;271(3):370–373. [PubMed] [Google Scholar]
- Robert-Baudouy J., Jimeno-Abendano J., Stoeber F. Individualité des hydro-lyases mannonique et altronique chez Escherichia coli K 12. C R Acad Sci Hebd Seances Acad Sci D. 1971 May 24;272(21):2740–2743. [PubMed] [Google Scholar]
- Taylor A. L. Current linkage map of Escherichia coli. Bacteriol Rev. 1970 Jun;34(2):155–175. doi: 10.1128/br.34.2.155-175.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zablotny R., Fraenkel D. G. Glucose and gluconate metabolism in a mutant of Escherichia coli lacking gluconate-6-phosphate dehydrase. J Bacteriol. 1967 May;93(5):1579–1581. doi: 10.1128/jb.93.5.1579-1581.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]