Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1972 Jan;109(1):134–139. doi: 10.1128/jb.109.1.134-139.1972

Mutants of Escherichia coli K-12 Blocked in the Final Reaction of Ubiquinone Biosynthesis: Characterization and Genetic Analysis

P Stroobant 1, I G Young 1, F Gibson 1
PMCID: PMC247260  PMID: 4333375

Abstract

The ubiquinone precursor 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1, 4-benzoquinone was isolated from two ubiquinone-deficient mutants of Escherichia coli and identified by nuclear magnetic resonance and mass spectrometry. The results of genetic analysis of the mutants indicate that each mutant carries a mutation in a gene designated ubiG which was located, by cotransduction with the nalA and glpT genes, at minute 42 on the E. coli chromosome.

Full text

PDF
135

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cox G. B., Gibson F., Pittard J. Mutant strains of Escherichia coli K-12 unable to form ubiquinone. J Bacteriol. 1968 May;95(5):1591–1598. doi: 10.1128/jb.95.5.1591-1598.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cox G. B., Young I. G., McCann L. M., Gibson F. Biosynthesis of ubiquinone in Escherichia coli K-12: location of genes affecting the metabolism of 3-octaprenyl-4-hydroxybenzoic acid and 2-octaprenylphenol. J Bacteriol. 1969 Aug;99(2):450–458. doi: 10.1128/jb.99.2.450-458.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cozzarelli N. R., Freedberg W. B., Lin E. C. Genetic control of L-alpha-glycerophosphate system in Escherichia coli. J Mol Biol. 1968 Feb 14;31(3):371–387. doi: 10.1016/0022-2836(68)90415-4. [DOI] [PubMed] [Google Scholar]
  4. Friis P., Nilsson J. L., Daves G. D., Jr, Folkers K. New multiprenylquinones in the biosynthesis of ubiquinone. Biochem Biophys Res Commun. 1967 Aug 7;28(3):324–327. doi: 10.1016/0006-291x(67)90312-9. [DOI] [PubMed] [Google Scholar]
  5. Hane M. W., Wood T. H. Escherichia coli K-12 mutants resistant to nalidixic acid: genetic mapping and dominance studies. J Bacteriol. 1969 Jul;99(1):238–241. doi: 10.1128/jb.99.1.238-241.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jackman L. M., O'Brien I. G., Cox G. B., Gibson F. Methionine as the source of methyl groups for ubiquinone and vitamin K: a study using nuclear magnetic resonance and mass spectrometry. Biochim Biophys Acta. 1967 Jun 13;141(1):1–7. doi: 10.1016/0304-4165(67)90239-5. [DOI] [PubMed] [Google Scholar]
  7. MONOD J., COHEN-BAZIRE G., COHN M. Sur la biosynthèse de la beta-galactosidase (lactase) chez Escherichia coli; la spécificité de l'induction. Biochim Biophys Acta. 1951 Nov;7(4):585–599. doi: 10.1016/0006-3002(51)90072-8. [DOI] [PubMed] [Google Scholar]
  8. PITTARD J. EFFECT OF INTEGRATED SEX FACTOR ON TRANSDUCTION OF CHROMOSOMAL GENES IN ESCHERICHIA COLI. J Bacteriol. 1965 Mar;89:680–686. doi: 10.1128/jb.89.3.680-686.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. TAYLOR A. L., THOMAN M. S. THE GENETIC MAP OF ESCHERICHIA COLI K-12. Genetics. 1964 Oct;50:659–677. doi: 10.1093/genetics/50.4.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Taylor A. L. Current linkage map of Escherichia coli. Bacteriol Rev. 1970 Jun;34(2):155–175. doi: 10.1128/br.34.2.155-175.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Taylor A. L., Trotter C. D. Revised linkage map of Escherichia coli. Bacteriol Rev. 1967 Dec;31(4):332–353. doi: 10.1128/br.31.4.332-353.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wilczynski J. J., Daves G. D., Jr, Folkers K. Structure determination of ubiquinone analogs by solvent shifts in nuclear magnetic resonance spectra. J Am Chem Soc. 1968 Sep 25;90(20):5593–5598. doi: 10.1021/ja01022a051. [DOI] [PubMed] [Google Scholar]
  13. Young I. G., McCann L. M., Stroobant P., Gibson F. Characterization and genetic analysis of mutant strains of Escherichia coli K-12 accumulating the biquinone precursors 2-octaprenyl-6-methoxy-1,4-benzoquinone and 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone. J Bacteriol. 1971 Mar;105(3):769–778. doi: 10.1128/jb.105.3.769-778.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES