Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1972 Jan;109(1):229–235. doi: 10.1128/jb.109.1.229-235.1972

Response of Escherichia coli B/r to High Concentrations of Sucrose in a Nutrient Medium

Paul O Scheie 1, Rita Rehberg 1
PMCID: PMC247271  PMID: 4550664

Abstract

Escherichia coli B/r was subjected to sucrose concentrations up to 1 m in the presence of Nutrient Broth. Plasmolysis seldom was evident 2 min after this treatment. The subsequent response was characterized by transient decreases in optical density as well as changes in appearance as seen under phase optics. No transient effects were detected in the synthetic rates or in the division of the survivors.

Full text

PDF
233

Images in this article

230Image
on p.230

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AVI-DOR Y., KUCZYNSKI M., SCHATZBERG G., MAGER J. Turbidity changes in bacterial suspensions: kinetics and relation to metabolic state. J Gen Microbiol. 1956 Feb;14(1):76–83. doi: 10.1099/00221287-14-1-76. [DOI] [PubMed] [Google Scholar]
  2. BERNHEIM F. Factors which affect the size of the organisms and the optical density of suspensions of Pseudomonas aeruginosa and Escherichia coli. J Gen Microbiol. 1963 Jan;30:53–58. doi: 10.1099/00221287-30-1-53. [DOI] [PubMed] [Google Scholar]
  3. Bayer M. E. Areas of adhesion between wall and membrane of Escherichia coli. J Gen Microbiol. 1968 Oct;53(3):395–404. doi: 10.1099/00221287-53-3-395. [DOI] [PubMed] [Google Scholar]
  4. Bayer M. E. Response of Cell Walls of Escherichia coli to a Sudden Reduction of the Environmental Osmotic Pressure. J Bacteriol. 1967 Mar;93(3):1104–1112. doi: 10.1128/jb.93.3.1104-1112.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Birdsell D. C., Cota-Robles E. H. Production and ultrastructure of lysozyme and ethylenediaminetetraacetate-lysozyme spheroplasts of Escherichia coli. J Bacteriol. 1967 Jan;93(1):427–437. doi: 10.1128/jb.93.1.427-437.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. COTA-ROBLES E. H. ELECTRON MICROSCOPY OF PLASMOLYSIS IN ESCHERICHIA COLI. J Bacteriol. 1963 Mar;85:499–503. doi: 10.1128/jb.85.3.499-503.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gros F., Gallant J., Weisberg R., Cashel M. Decryptification of RNA polymerase in whole cells of Escherichia coli. J Mol Biol. 1967 May 14;25(3):555–557. doi: 10.1016/0022-2836(67)90206-9. [DOI] [PubMed] [Google Scholar]
  8. HENNEMAN D. H., UMBREIT W. W. INFLUENCE OF THE PHYSICAL STATE OF THE BACTERIAL CELL MEMBRANE UPON THE RATE OF RESPIRATION. J Bacteriol. 1964 Jun;87:1274–1280. doi: 10.1128/jb.87.6.1274-1280.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hurwitz C., Braun C. B., Peabody R. A. Washing bacteria by centrifugation through a water-immiscible layer of silicones. J Bacteriol. 1965 Dec;90(6):1692–1695. doi: 10.1128/jb.90.6.1692-1695.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MAGER J., KUCZYNSKI M., SCHATZBERG G., AVI-DOR Y. Turbidity changes in bacterial suspensions in relation to osmotic pressure. J Gen Microbiol. 1956 Feb;14(1):69–75. doi: 10.1099/00221287-14-1-69. [DOI] [PubMed] [Google Scholar]
  11. MASON D. J., POWELSON D. M. Nuclear division as observed in live bacteria by a new technique. J Bacteriol. 1956 Apr;71(4):474–479. doi: 10.1128/jb.71.4.474-479.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Myers W. F., Provost P. J., Wisseman C. L., Jr Permeability properties of Rickettsia mooseri. J Bacteriol. 1967 Mar;93(3):950–960. doi: 10.1128/jb.93.3.950-960.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Okrend A. G., Doetsch R. N. Plasmolysis and bacterial motility: a method for the study of membrane function. Arch Mikrobiol. 1969;69(1):69–78. doi: 10.1007/BF00408565. [DOI] [PubMed] [Google Scholar]
  14. PACKER L., PERRY M. Energy-linked light-scattering changes in Escherichia coli. Arch Biochem Biophys. 1961 Nov;95:379–388. doi: 10.1016/0003-9861(61)90163-1. [DOI] [PubMed] [Google Scholar]
  15. ROGERS D., YU S. H. TURBIDITY CHANGE DURING GLUCOSE PERMEATION IN ESCHERICHIA COLI. J Bacteriol. 1963 May;85:1141–1149. doi: 10.1128/jb.85.5.1141-1149.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rubenstein K. E., Nass M. M., Cohen S. S. Synthetic capabilities of plasmolyzed cells and spheroplasts of Escherichia coli. J Bacteriol. 1970 Oct;104(1):443–452. doi: 10.1128/jb.104.1.443-452.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. SCHAECHTER M., WILLIAMSON J. P., HOOD J. R., Jr, KOCH A. L. Growth, cell and nuclear divisions in some bacteria. J Gen Microbiol. 1962 Nov;29:421–434. doi: 10.1099/00221287-29-3-421. [DOI] [PubMed] [Google Scholar]
  18. SISTROM W. R. On the physical state of the intracellularly accumulates substrates of beta-galactoside-permease in Escherichia coli. Biochim Biophys Acta. 1958 Sep;29(3):579–587. doi: 10.1016/0006-3002(58)90015-5. [DOI] [PubMed] [Google Scholar]
  19. STEMPEN H. Demonstration of the chromatinic bodies of Escherichia coli and Proteus vulgaris with the aid of the phase contrast microscope. J Bacteriol. 1950 Jul;60(1):81–87. doi: 10.1128/jb.60.1.81-87.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Scheie P. O. Plasmolysis of Escherichia coli B-r with sucrose. J Bacteriol. 1969 May;98(2):335–340. doi: 10.1128/jb.98.2.335-340.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Scheie P., Dalen H. Spatial anisotropy in Escherichia coli. J Bacteriol. 1968 Oct;96(4):1413–1414. doi: 10.1128/jb.96.4.1413-1414.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES