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. 1972 Apr;110(1):47–55. doi: 10.1128/jb.110.1.47-55.1972

Mutagen Stability of Alkylation-Sensitive Mutants of Bacillus subtilis

T Hill a, L Prakash a,1, B Strauss a
PMCID: PMC247377  PMID: 4622905

Abstract

A series of mutations of Bacillus subtilis, conferring sensitivity to methyl methanesulfonate (MMS), were transferred by transformation to a suppressible his− stock. The introduction of certain sensitivity mutations prevented the ultraviolet- or MMS-induced, but not the spontaneous, reversion of his− to his+. Not all sensitivity mutations led to this resistance to mutagenesis; a strain with altered deoxyribonucleic acid (DNA) polymerase activity behaved almost normally with respect to its mutagen response, as did an excision-defective, ultraviolet-sensitive strain used as a control. One of the mutagen-stable strains responded to mutagenesis with nitrosomethylguanidine; another appeared stable even to this mutagen. All mutagen-stable strains had DNA polymerase and DNA ligase activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cozzarelli N. R., Kelly R. B., Kornberg A. A minute circular DNA from Escherichia coli 15. Proc Natl Acad Sci U S A. 1968 Jul;60(3):992–999. doi: 10.1073/pnas.60.3.992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gass K. B., Hill T. C., Goulian M., Strauss B. S., Cozzarelli N. R. Altered deoxyribonucleic acid polymerase activity in a methyl methanesulfonate-sensitive mutant of Bacillus subtilis. J Bacteriol. 1971 Oct;108(1):364–374. doi: 10.1128/jb.108.1.364-374.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hoch J. A., Anagnostopoulos C. Chromosomal location and properties of radiation sensitivity mutations in Bacillus subtilis. J Bacteriol. 1970 Aug;103(2):295–301. doi: 10.1128/jb.103.2.295-301.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kelly R. B., Cozzarelli N. R., Deutscher M. P., Lehman I. R., Kornberg A. Enzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break. J Biol Chem. 1970 Jan 10;245(1):39–45. [PubMed] [Google Scholar]
  5. Kondo S., Ichikawa H., Iwo K., Kato T. Base-change mutagenesis and prophage induction in strains of Escherichia coli with different DNA repair capacities. Genetics. 1970 Oct;66(2):187–217. doi: 10.1093/genetics/66.2.187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mahler I. Effect of mitomycin C on five excision-repair mutants of Bacillus subtilis. Biochem Biophys Res Commun. 1966 Oct 5;25(1):73–79. doi: 10.1016/0006-291x(66)90642-5. [DOI] [PubMed] [Google Scholar]
  7. Okubo S., Romig W. R. Impaired transformability of Bacillus subtilis mutant sensitive to mitomycin C and ultraviolet radiation. J Mol Biol. 1966 Feb;15(2):440–454. doi: 10.1016/s0022-2836(66)80120-1. [DOI] [PubMed] [Google Scholar]
  8. Okubo S., Yanagida T. Isolation of a suppressor mutant in Bacillus subtilis. J Bacteriol. 1968 Mar;95(3):1187–1188. doi: 10.1128/jb.95.3.1187-1188.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Prakash L., Strauss B. Repair of alkylation damage: stability of methyl groups in Bacillus subtilis treated with methyl methanesulfonate. J Bacteriol. 1970 Jun;102(3):760–766. doi: 10.1128/jb.102.3.760-766.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Reiter H., Strauss B. Repair of damage induced by a monofunctional alkylating agent in a transformable, ultraviolet-sensitive strain of Bacillus subtilis. J Mol Biol. 1965 Nov;14(1):179–194. doi: 10.1016/s0022-2836(65)80239-x. [DOI] [PubMed] [Google Scholar]
  11. SAITO H., MIURA K. I. PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT. Biochim Biophys Acta. 1963 Aug 20;72:619–629. [PubMed] [Google Scholar]
  12. STRAUSS B. S. Differential destruction of the transforming activity of damaged deoxyribonucleic acid by a bacterial enzyme. Proc Natl Acad Sci U S A. 1962 Sep 15;48:1670–1675. doi: 10.1073/pnas.48.9.1670. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Searashi T., Strauss B. Relation of the repair of damage induced by a monofunctional alkylating agent to the repair of damage induced by ultraviolet light in Bacillus subtilis. Biochem Biophys Res Commun. 1965 Sep 22;20(6):680–687. doi: 10.1016/0006-291x(65)90069-0. [DOI] [PubMed] [Google Scholar]
  14. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wilson G. A., Bott K. F. Nutritional factors influencing the development of competence in the Bacillus subtilis transformation system. J Bacteriol. 1968 Apr;95(4):1439–1449. doi: 10.1128/jb.95.4.1439-1449.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

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