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. 1972 Apr;110(1):56–65. doi: 10.1128/jb.110.1.56-65.1972

Induction of the ara Operon of Escherichia coli B/r

M Ellin Doyle 1, Candice Brown 1, Robert W Hogg 1, Robert B Helling 1
PMCID: PMC247378  PMID: 4553005

Abstract

The inducer specificity and kinetics of induction of the ara operon were examined in Escherichia coli B/r. A difference in the kinetics of induction was found between our B/r strains and the K-12 strain previously described by Schleif. The roles of active transport and metabolism of inducer, and of cell density, in induction were studied. d-Fucose and β-methyl-l-arabinoside were competitive inhibitors of induction. No inducer of the wild-type strain other than l-arabinose was found. However, a procedure for selecting mutants with altered inducer affinity or specificity was developed. The properties of one such mutant (inducible by d-fucose) are described.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler J. Chemoreceptors in bacteria. Science. 1969 Dec 26;166(3913):1588–1597. doi: 10.1126/science.166.3913.1588. [DOI] [PubMed] [Google Scholar]
  2. Beverin S., Sheppard D. E., Park S. S. D-Fucose as a gratuitous inducer of the L-arabinose operon in strains of Escherichia coli B-r mutant in gene araC. J Bacteriol. 1971 Jul;107(1):79–86. doi: 10.1128/jb.107.1.79-86.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bhattacharya A. K., Chakravorty M. Induction and repression of L-arabinose isomerase in Salmonella typhimurium. J Bacteriol. 1971 Apr;106(1):107–112. doi: 10.1128/jb.106.1.107-112.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. COHEN S. Studies on D-ribulose and its enzymatic conversion to D-arabinose. J Biol Chem. 1953 Mar;201(1):71–84. [PubMed] [Google Scholar]
  5. DISCHE Z., BORENFREUND E. A new spectrophotometric method for the detection and determination of keto sugars and trioses. J Biol Chem. 1951 Oct;192(2):583–587. [PubMed] [Google Scholar]
  6. ENGLESBERG E. Enzymatic characterization of 17 L-arabinose negative mutants of Escherichia coli. J Bacteriol. 1961 Jun;81:996–1006. doi: 10.1128/jb.81.6.996-1006.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Englesberg E., Irr J., Power J., Lee N. Positive control of enzyme synthesis by gene C in the L-arabinose system. J Bacteriol. 1965 Oct;90(4):946–957. doi: 10.1128/jb.90.4.946-957.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Englesberg E., Sheppard D., Squires C., Meronk F., Jr An analysis of "revertants" of a deletion mutant in the C gene of the L-arabinose gene complex in Escherichia coli B-r: isolation of initiator constitutive mutants (Ic). J Mol Biol. 1969 Jul 28;43(2):281–298. doi: 10.1016/0022-2836(69)90268-x. [DOI] [PubMed] [Google Scholar]
  9. Englesberg E., Squires C., Meronk F., Jr The L-arabinose operon in Escherichia coli B-r: a genetic demonstration of two functional states of the product of a regulator gene. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1100–1107. doi: 10.1073/pnas.62.4.1100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fuleki T., Francis F. J. A new developing solvent for paper chromatography of various phenolic compounds, sugars and amino acids. J Chromatogr. 1967 Feb;26(2):404–411. doi: 10.1016/s0021-9673(01)98897-1. [DOI] [PubMed] [Google Scholar]
  11. Ganesan A. K., Rotman B. Transport systems for galactose and galactosides in Escherichia coli. I. Genetic determination and regulation of the methyl-galactoside permease. J Mol Biol. 1966 Mar;16(1):42–50. doi: 10.1016/s0022-2836(66)80261-9. [DOI] [PubMed] [Google Scholar]
  12. Greenblatt J., Schleif R. Arabinose C protein: regulation of the arabinose operon in vitro. Nat New Biol. 1971 Oct 6;233(40):166–170. doi: 10.1038/newbio233166a0. [DOI] [PubMed] [Google Scholar]
  13. HELLING R. B., WEINBERG R. COMPLEMENTATION STUDIES OF ARABINOSE GENES IN ESCHERICHIA COLI. Genetics. 1963 Oct;48:1397–1410. doi: 10.1093/genetics/48.10.1397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Helling R. B. Selection of a mutant of Escherichia coli which has high mutation rates. J Bacteriol. 1968 Oct;96(4):975–980. doi: 10.1128/jb.96.4.975-980.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Helling R. B. The effect of arabinose-specific enzyme synthesis on recombination in the arabinose genes of Escherichia coli. Genetics. 1967 Nov;57(3):665–675. doi: 10.1093/genetics/57.3.665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hogg R. W., Englesberg E. L-arabinose binding protein from Escherichia coli B-r. J Bacteriol. 1969 Oct;100(1):423–432. doi: 10.1128/jb.100.1.423-432.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Katz L., Englesberg E. Hyperinducibility as a result of mutation in structural genes and self-catabolite repression in the ara operon. J Bacteriol. 1971 Jul;107(1):34–52. doi: 10.1128/jb.107.1.34-52.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. LEE N., ENGLESBERG E. COORDINATE VARIATIONS IN INDUCED SYNTHESES OF ENZYMES ASSOCIATED WITH MUTATIONS IN A STRUCTURAL GENE. Proc Natl Acad Sci U S A. 1963 Oct;50:696–702. doi: 10.1073/pnas.50.4.696. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  20. Lee N., Bendet I. Crystalline L-ribulokinase from Escherichia coli. J Biol Chem. 1967 May 10;242(9):2043–2050. [PubMed] [Google Scholar]
  21. Novotny C. P., Englesberg E. The L-arabinose permease system in Escherichia coli B/r. Biochim Biophys Acta. 1966 Mar 28;117(1):217–230. doi: 10.1016/0304-4165(66)90169-3. [DOI] [PubMed] [Google Scholar]
  22. PALLERONI N. J., DOUDOROFF M. Mannose isomerase of Pseudomonas saccharophila. J Biol Chem. 1956 Jan;218(1):535–548. [PubMed] [Google Scholar]
  23. Patrick J. W., Lee N., Barnes N. B., Englesberg E. Coordination of enzyme synthesis in the L-arabinose operon in Escherichia coli. I. The effect of manganous ion on the synthesis of L-arabinose isomerase. J Biol Chem. 1971 Aug 25;246(16):5102–5106. [PubMed] [Google Scholar]
  24. Rotman B., Ganesan A. K., Guzman R. Transport systems for galactose and galactosides in Escherichia coli. II. Substrate and inducer specificities. J Mol Biol. 1968 Sep 14;36(2):247–260. doi: 10.1016/0022-2836(68)90379-3. [DOI] [PubMed] [Google Scholar]
  25. Schleif R. An L-arabinose binding protein and arabinose permeation in Escherichia coli. J Mol Biol. 1969 Nov 28;46(1):185–196. doi: 10.1016/0022-2836(69)90065-5. [DOI] [PubMed] [Google Scholar]
  26. Schleif R., Greenblatt J., Davis R. W. Dual control of arabinose genes on transducing phage lambda-dara. J Mol Biol. 1971 Jul 14;59(1):127–150. doi: 10.1016/0022-2836(71)90417-7. [DOI] [PubMed] [Google Scholar]
  27. Schleif R. Induction of the L-arabinose operon. J Mol Biol. 1969 Nov 28;46(1):197–199. doi: 10.1016/0022-2836(69)90066-7. [DOI] [PubMed] [Google Scholar]
  28. Sheppard D. E., Englesberg E. Further evidence for positive control of the L-arabinose system by gene araC. J Mol Biol. 1967 May 14;25(3):443–454. doi: 10.1016/0022-2836(67)90197-0. [DOI] [PubMed] [Google Scholar]
  29. Wilcox G., Singer J., Heffernan L. Deoxyribonucleic acid-ribonucleic acid hybridization studies on the L-Arabinose operon of Escherichia coli B-r. J Bacteriol. 1971 Oct;108(1):1–4. doi: 10.1128/jb.108.1.1-4.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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