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. 1972 Apr;110(1):179–185. doi: 10.1128/jb.110.1.179-185.1972

Stimulation of Unbalanced Ribonucleic Acid Synthesis in Escherichia coli by Methanol

George T Javor 1
PMCID: PMC247396  PMID: 4552987

Abstract

Addition of methanol to a stringent strain of Escherichia coli, starving of methionine, stimulates unbalanced ribonucleic acid (RNA) synthesis. The newly formed RNA and ribonucleoprotein species sediment between 4S and 30S. As a result of methanol treatment, cells become permeable to actinomycin D. Damage to cellular membrane appears to influence the control for RNA synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARONSON A. I., SPIEGELMAN S. Protein and ribonucleic acid synthesis in a chloramphenicol-inhibited system. Biochim Biophys Acta. 1961 Oct 14;53:70–84. doi: 10.1016/0006-3002(61)90795-8. [DOI] [PubMed] [Google Scholar]
  2. BOREK E., RYAN A., ROCKENBACH J. Nucleic acid metabolism in relation to the lysogenic phenomenon. J Bacteriol. 1955 Apr;69(4):460–467. doi: 10.1128/jb.69.4.460-467.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cashel M., Gallant J. Control of RNA synthesis in Escherichia coli. I. Amino acid dependence of the synthesis of the substrates of RNA polymerase. J Mol Biol. 1968 Jul 14;34(2):317–330. doi: 10.1016/0022-2836(68)90256-8. [DOI] [PubMed] [Google Scholar]
  4. Cashel M., Gallant J. Two compounds implicated in the function of the RC gene of Escherichia coli. Nature. 1969 Mar 1;221(5183):838–841. doi: 10.1038/221838a0. [DOI] [PubMed] [Google Scholar]
  5. Cashel M., Kalbacher B. The control of ribonucleic acid synthesis in Escherichia coli. V. Characterization of a nucleotide associated with the stringent response. J Biol Chem. 1970 May 10;245(9):2309–2318. [PubMed] [Google Scholar]
  6. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dalgarno L., Gros F. RNA synthesis in "relaxed" and "stringent" Escherichia coli. Breakdown of performed ribonucleoprotein particles and subsequent RNA synthesis. Biochim Biophys Acta. 1968 Mar 18;157(1):64–75. [PubMed] [Google Scholar]
  8. Edlin G., Stent G. S. Nucleoside triphosphate pools and the regulation of RNA synthesis in E. coli. Proc Natl Acad Sci U S A. 1969 Feb;62(2):475–482. doi: 10.1073/pnas.62.2.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ezekiel D. H., Elkins B. N. The stimulation of ribonucleic acid synthesis by ribosome inhibitors in amino acid-starved Escherichia coli. Biochim Biophys Acta. 1968 Sep 24;166(2):466–474. doi: 10.1016/0005-2787(68)90234-7. [DOI] [PubMed] [Google Scholar]
  10. Gallant J., Cashel M. On the mechanism of amino acid control of ribonucleic acid biosynthesis. J Mol Biol. 1967 May 14;25(3):545–553. doi: 10.1016/0022-2836(67)90205-7. [DOI] [PubMed] [Google Scholar]
  11. Gallant J., Harada B. The control of ribonucleic acid synthesis in Escherichia coli. 3. The functional relationship between purine ribonucleoside triphosphate pool sizes and the rate of ribonucleic acid accumulation. J Biol Chem. 1969 Jun 25;244(12):3125–3132. [PubMed] [Google Scholar]
  12. Harshman R. B., Yamazaki H. Formation of ppGpp in a relaxed and stringent strain of Escherichia coli during diauxie lag. Biochemistry. 1971 Oct 12;10(21):3980–3982. doi: 10.1021/bi00797a027. [DOI] [PubMed] [Google Scholar]
  13. Irr J., Gallant J. The control of ribonucleic acid synthesis in Escherichia coli. II. Stringent control of energy metabolism. J Biol Chem. 1969 Apr 25;244(8):2233–2239. [PubMed] [Google Scholar]
  14. Nakada D., Marquisee M. J. Relaxed synthesis of ribosomal RNA by a stringent strain of Escherichia coli. J Mol Biol. 1965 Sep;13(2):351–361. doi: 10.1016/s0022-2836(65)80102-4. [DOI] [PubMed] [Google Scholar]
  15. PARDEE A. B., PRESTIDGE L. S. The dependence of nucleic acid synthesis on the presence of amino acids in Escherichia coli. J Bacteriol. 1956 Jun;71(6):677–683. doi: 10.1128/jb.71.6.677-683.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Peterson R. L., Radcliffe C. W., Pace N. R. Ribonucleic acid synthesis in bacteria treated with toluene. J Bacteriol. 1971 Aug;107(2):585–588. doi: 10.1128/jb.107.2.585-588.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Raina A., Cohen S. S. Polyamines and RNA synthesis in a polyauxotrophic strain of E. coli. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1587–1593. doi: 10.1073/pnas.55.6.1587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Raina A., Jansen M., Cohen S. S. Polyamines and the accumulation of ribonucleic acid in some polyauxotrophic strains of Escherichia coli. J Bacteriol. 1967 Nov;94(5):1684–1696. doi: 10.1128/jb.94.5.1684-1696.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. STENT G. S., BRENNER S. A genetic locus for the regulation of ribonucleic acid synthesis. Proc Natl Acad Sci U S A. 1961 Dec 15;47:2005–2014. doi: 10.1073/pnas.47.12.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sokawa Y., Kaziro Y. Amino acid-dependent control of the transport of alpha-methyl glucoside in E. coli. Biochem Biophys Res Commun. 1969 Jan 6;34(1):99–103. doi: 10.1016/0006-291x(69)90534-8. [DOI] [PubMed] [Google Scholar]
  21. Sokawa Y., Nakao-Sato E., Kaziro Y. RC gene control in Escherichia coli is not restricted to RNA synthesis. Biochim Biophys Acta. 1970 Jan 21;199(1):256–264. doi: 10.1016/0005-2787(70)90714-8. [DOI] [PubMed] [Google Scholar]
  22. Tremblay G. Y., Daniels M. J., Schaechter M. Isolation of a cell membrane-DNA-nascent RNA complex from bacteria. J Mol Biol. 1969 Feb 28;40(1):65–76. doi: 10.1016/0022-2836(69)90296-4. [DOI] [PubMed] [Google Scholar]
  23. Tropp B. E., Meade L. C., Thomas P. J. Consequences of expression of the "relaxed" genotype of the RC gene. Lipid synthesis. J Biol Chem. 1970 Feb 25;245(4):855–858. [PubMed] [Google Scholar]
  24. Willis D. B., Ennis H. L. Potassium requirement for synthesis of macromolecules in Bacillus subtilis infected with bacteriophage 2C. J Virol. 1969 Jan;3(1):1–7. doi: 10.1128/jvi.3.1.1-7.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wong J. T., Nazar R. N. Relationship of the MS nucleotides to the regulation of ribonucleic acid synthesis in Escherichia coli. J Biol Chem. 1970 Sep 10;245(17):4591–4593. [PubMed] [Google Scholar]

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