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. 1972 May;110(2):722–730. doi: 10.1128/jb.110.2.722-730.1972

Detection of Cholesterol in Cell Membranes by Use of Bacterial Toxins

I R Pendleton a,1, K S Kim a, A W Bernheimer a
PMCID: PMC247469  PMID: 4623312

Abstract

A method is described for the detection of cholesterol in membranes from erythrocytes, mycoplasmas, and bacterial cells by a ferritin-labeling technique. Membranes treated with cereolysin, a bacterial hemolysin which specifically binds to cholesterol, and then treated with ferritin-antitetanolysin, were specifically ferritin-labeled for cholesterol. A similar antigen-antibody system, streptolysin O-ferritin-antistreptolysin, was also used successfully with erythrocyte membranes. There was an uneven distribution of ferritin in erythrocyte membranes suggesting that the distribution of cholesterol may not be entirely random. Mycoplasma gallisepticum was intensely labeled, but Acholeplasma laidlawii with or without cholesterol in the membranes was not labeled, suggesting an unusual location for cholesterol in A. laidlawii membranes. As controls, two of three species of bacterial membranes lacking cholesterol were not ferritin-labeled.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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