Abstract
Protoplast formation by Histoplasma capsulatum yeasts using high concentrations of MgSO4 occurs either by lysis of the bud or lysis of the entire cell wall. Both mechanisms may also occur simultaneously. Neither the protoplast emerging through a hole in the cell wall nor the freshly released protoplast has a recognizable cell wall or the remnant of such. The protoplast contains all the organelles of the normal cell except for mesosomes. During protoplast formation the nucleus increases in size and produces several nuclear masses by the invaginations of the internal layer of the nuclear membrane. All these nuclear masses are surrounded by the external layer of the nuclear membrane. Several nuclei with a normal nuclear membrane are formed later.
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- Avers C. J., Federman M. The occurrence in yeast of cytoplasmic granules which resemble microbodies. J Cell Biol. 1968 May;37(2):555–559. doi: 10.1083/jcb.37.2.555. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Berliner M. D., Reca M. E. Protoplasts of systemic dimorphic fungal pathogens: Histoplasma capsulatum and Blastomyces dermatitidis. Mycopathol Mycol Appl. 1969 Jan 29;37(1):81–85. doi: 10.1007/BF02051335. [DOI] [PubMed] [Google Scholar]
- Berliner M. D., Reca M. E. Release of protoplasts in the yeast phase of Histoplasma capsulatum without added enzyme. Science. 1970 Feb 27;167(3922):1255–1257. doi: 10.1126/science.167.3922.1255. [DOI] [PubMed] [Google Scholar]
- Carbonell L. M. Intrayeast hyphae in Paracoccidioides brasiliensis. J Bacteriol. 1969 Jun;98(3):1395–1396. doi: 10.1128/jb.98.3.1395-1396.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Darling S., Theilade J., Birch-Andersen A. Kinetic and morphological observations on Saccharomyces cerevisiae during spheroplast formation. J Bacteriol. 1969 May;98(2):797–810. doi: 10.1128/jb.98.2.797-810.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EDWARDS M. R., HAZEN E. L., EDWARDS G. A. The fine structure of the yeast-like cells of Histoplasma in culture. J Gen Microbiol. 1959 Jun;20(3):496–503. doi: 10.1099/00221287-20-3-496. [DOI] [PubMed] [Google Scholar]
- FREEMAN J. A., SPURLOCK B. O. A new epoxy embedment for electron microscopy. J Cell Biol. 1962 Jun;13:437–443. doi: 10.1083/jcb.13.3.437. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leiva S., Carbonell L. M. Demonstration of succinic dehydrogenase in the mesosomes of the mycelial phase of Paracoccidiosides brasiliensis. J Gen Microbiol. 1970 Jul;62(1):43–47. doi: 10.1099/00221287-62-1-43. [DOI] [PubMed] [Google Scholar]
- Necas O. Mechanism of regeneration of yeast protoplasts. 3. Electron microscopy of growing protoplasts. Folia Biol (Praha) 1965;11(5):371–377. [PubMed] [Google Scholar]
- REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rost K. Gewinnung und Eignschaften von Protoplasten aus Schizosaccharomyces pombe. Z Allg Mikrobiol. 1969;9(4):290–295. [PubMed] [Google Scholar]
- WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol. 1958 Jul 25;4(4):475–478. doi: 10.1083/jcb.4.4.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WEIBULL C. The isolation of protoplasts from Bacillus megaterium by controlled treatment with lysozyme. J Bacteriol. 1953 Dec;66(6):688–695. doi: 10.1128/jb.66.6.688-695.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wilsenach R., Kessel M. The role of lomasomes in wall formation in Penicillium vermiculatum. J Gen Microbiol. 1965 Sep;40(3):401–404. doi: 10.1099/00221287-40-3-401. [DOI] [PubMed] [Google Scholar]