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. 1972 Jun;110(3):857–863. doi: 10.1128/jb.110.3.857-863.1972

Deletion Map of the Escherichia coli K-12 Sex Factor F: the Order of Eleven Transfer Cistrons

K Ippen-Ihler a,1, M Achtman a,2, N Willetts a
PMCID: PMC247503  PMID: 4555414

Abstract

A series of Hfr deletion mutants was isolated. These mutants contain deletions which extend from a λ prophage into an Flac which is integrated into the gal operon. Transfer-deficient deletion mutants were found to fall into four different phenotypic groups when tested for male- and female-specific phage resistance. Conjugational and transductional complementation tests with Flac point mutants deficient in transfer (tra−) were performed, and the order of 11 tra cistrons was determined. The tra genes are all located between an F gene for the inhibition of female-specific phages and the transposed lac operon originally carried by the Flac. The order of genes in the Hfr studied was established to be: proC... φIIR... traJ traA traE traK traB traC traF traH traG traD traI...lac...attλ...bio.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Achtman M. A semi-automatic technique for conducting many bacterial matings concurrently. Genet Res. 1971 Jun;17(3):261–266. doi: 10.1017/s0016672300012283. [DOI] [PubMed] [Google Scholar]
  2. Achtman M., Willetts N., Clark A. J. Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants. J Bacteriol. 1971 May;106(2):529–538. doi: 10.1128/jb.106.2.529-538.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Achtman M., Willetts N., Clark A. J. Conjugational complementation analysis of transfer-deficient mutants of Flac in Escherichia coli. J Bacteriol. 1972 Jun;110(3):831–842. doi: 10.1128/jb.110.3.831-842.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cuzin F. Un bactériophage spécifique du type sexuel F- d'Escherichia coli K 12. C R Acad Sci Hebd Seances Acad Sci D. 1965 Jun 14;260(24):6482–6485. [PubMed] [Google Scholar]
  5. Eisen H., Pereira da Silva L., Jacob F. The regulation and mechanism of DNA synthesis in bacteriophage lambda. Cold Spring Harb Symp Quant Biol. 1968;33:755–764. doi: 10.1101/sqb.1968.033.01.086. [DOI] [PubMed] [Google Scholar]
  6. Finnegan D. J., Willetts N. S. Two classes of Flac mutants insensitive to transfer inhibition by an F-like R factor. Mol Gen Genet. 1971;111(3):256–264. doi: 10.1007/BF00433110. [DOI] [PubMed] [Google Scholar]
  7. HAKURA A., OTSUJI N., HIROTA Y. A TEMPERATE PHAGE SPECIFIC FOR FEMALE STRAINS OF ESCHERICHIA COLI K 12. J Gen Microbiol. 1964 Apr;35:69–73. doi: 10.1099/00221287-35-1-69. [DOI] [PubMed] [Google Scholar]
  8. Ippen K., Shapiro J. A., Beckwith J. R. Transposition of the lac region to the gal region of the Escherichia coli chromosome: isolation of lambda-lac transducing bacteriophages. J Bacteriol. 1971 Oct;108(1):5–9. doi: 10.1128/jb.108.1.5-9.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOEB T. Isolation of a bacteriophage specific for the F plus and Hfr mating types of Escherichia coli K-12. Science. 1960 Mar 25;131(3404):932–933. doi: 10.1126/science.131.3404.932. [DOI] [PubMed] [Google Scholar]
  10. LOEB T., ZINDER N. D. A bacteriophage containing RNA. Proc Natl Acad Sci U S A. 1961 Mar 15;47:282–289. doi: 10.1073/pnas.47.3.282. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MAEKELAE O., MAEKELAE P. H., SOIKKELI S. SEX-SPECIFICITY OF THE BACTERIOPHAGE T7. Ann Med Exp Biol Fenn. 1964;42:188–195. [PubMed] [Google Scholar]
  12. Morrison T. G., Malamy M. H. T7 translational control mechanisms and their inhibiton by F factors. Nat New Biol. 1971 May 12;231(19):37–41. doi: 10.1038/newbio231037a0. [DOI] [PubMed] [Google Scholar]
  13. Ohlsson B. M., Strigini P. F., Beckwith J. R. Allelic amber and ochre suppressors. J Mol Biol. 1968 Sep 14;36(2):209–218. doi: 10.1016/0022-2836(68)90376-8. [DOI] [PubMed] [Google Scholar]
  14. Otsubo E., Nishimura Y., Hirota Y. Transfer-defective mutants of sex factors in Escherichia coli. I. Defective mutants and complementation analysis. Genetics. 1970 Feb;64(2):173–188. doi: 10.1093/genetics/64.2.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Otsubo E. Transfer-defective mutants of sex factors in Escherichia coli. II. Deletion mutants of an F-prime and deletion mapping of cistrons involved in genetic transfer. Genetics. 1970 Feb;64(2):189–197. doi: 10.1093/genetics/64.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shapiro J. A., Adhya S. L. The galactose operon of E. coli K-12. II. A deletion analysis of operon structure and polarity. Genetics. 1969 Jun;62(2):249–264. doi: 10.1093/genetics/62.2.249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Silverman P. M., Mobach H. W., Valentine R. C. Sex hair (F-pili) mutants of E. coli. Biochem Biophys Res Commun. 1967 May 5;27(3):412–416. doi: 10.1016/s0006-291x(67)80115-3. [DOI] [PubMed] [Google Scholar]
  18. WATANABE T., OKADA M. NEW TYPE OF SEX FACTOR-SPECIFIC BACTERIOPHAGE OF ESCHERICHIA COLI. J Bacteriol. 1964 Mar;87:727–736. doi: 10.1128/jb.87.3.727-736.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Willetts N. S., Finnegan D. J. Characteristics of E. coli K12 strains carrying both an F prime and an R factor. Genet Res. 1970 Aug;16(1):113–122. doi: 10.1017/s0016672300002329. [DOI] [PubMed] [Google Scholar]
  20. Willetts N. S. Plasmid specificity of two proteins required for conjugation in E. coli K12. Nat New Biol. 1971 Apr 7;230(14):183–185. doi: 10.1038/newbio230183a0. [DOI] [PubMed] [Google Scholar]
  21. Willetts N., Achtman M. Genetic analysis of transfer by the Escherichia coli sex factor F, using P1 transductional complementation. J Bacteriol. 1972 Jun;110(3):843–851. doi: 10.1128/jb.110.3.843-851.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

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