Abstract
Heterogeneity in the distribution or binding of the membrane phospholipids was demonstrated in the membrane fragments released from Haemophilus parainfluenzae by treatment with ethylenediaminetetraacetic acid (EDTA)-tris(hydroxymethyl)-aminomethane (Tris). The membrane fragments released early in the EDTA-Tris treatment contained two- to fivefold higher proportions of cardiolipin and phosphatidylglycerol and less phosphatidylethanolamine as well as phospholipids with threefold lower specific activity of the phospholipid phosphate after a short pulse of 32P than were found in the residue. Heterogeneity was best demonstrated with shorter EDTA-Tris treatments and shorter periods of growth with 32P. EDTA-Tris treatment appeared to progressively strip phospholipids from the cells that were synthesized at progressively later times.
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Selected References
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