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. 1970 Jun;102(3):711–715. doi: 10.1128/jb.102.3.711-715.1970

Comparison of Lysyl-Transfer Ribonucleic Acid Species from Vegetative Cells and Spores of Bacillus subtilis by Methylated Albumin-Kieselguhr and Reversed-Phase Chromatography

Barbara S Vold 1
PMCID: PMC247616  PMID: 4317102

Abstract

Lysyl-transfer ribonucleic acid (tRNA) species from a spore-forming strain of Bacillus subtilis (168 trp2) and an early blocked asporogenous mutant (spoA 12) were compared on reversed-phase and methylated albumin-kieselguhr columns. Lysyl-tRNA species from spores and the asporogenous mutant in stationary phase both exhibited altered chromatographic profiles compared to that of log-phase cells. The major peak in spore lysyl-tRNA species eluted later than that characteristic of vegetative cells, whereas the major peak of the lysyl-tRNA species from the asporogenous mutant in stationary phase eluted earlier. Although the early eluting lysyl-tRNA species was observable on methylated albumin columns, the late eluting peak was not detectable by that column technique. By using a shallower gradient on an RPC-2 column, the resolution of all lysyl-tRNA species increased. Several subspecies were revealed. The chromatographic comparisons clearly show that both the spore-forming strain and the asporogenous mutant undergo relative increases in different lysyl-tRNA species when grown to late stationary phase. No new species seem to be involved but rather altered amounts of minor species existing in log-phase cells. The experiments also demonstrate the usefulness of reversed-phase columns for such comparisons.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Gefter M. L., Russell R. L. Role modifications in tyrosine transfer RNA: a modified base affecting ribosome binding. J Mol Biol. 1969 Jan 14;39(1):145–157. doi: 10.1016/0022-2836(69)90339-8. [DOI] [PubMed] [Google Scholar]
  2. Kaneko I., Doi R. H. Alteration of valyl-sRNA during sporulation of bacillus subtilis. Proc Natl Acad Sci U S A. 1966 Mar;55(3):564–571. doi: 10.1073/pnas.55.3.564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. LAZZARINI R. A., PETERKOFSKY A. THE CHARACTERIZATION OF A NEW SPECIES OF LEUCYL-SRNA FORMED DURING METHIONINE DEPRIVATION OF ESCHERICHIA COLI WITH RELAXED CONTROL. Proc Natl Acad Sci U S A. 1965 Mar;53:549–556. doi: 10.1073/pnas.53.3.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lazzarini R. A. Differences in lysine-sRNA from spore and vegetative cells of Bacillus subtillis. Proc Natl Acad Sci U S A. 1966 Jul;56(1):185–190. doi: 10.1073/pnas.56.1.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lazzarini R. A., Santangelo E. Medium-dependent alteration of lysine transfer ribonucleic acid in sporulating Bacillus subtilis cells. J Bacteriol. 1967 Jul;94(1):125–130. doi: 10.1128/jb.94.1.125-130.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. MANDELL J. D., HERSHEY A. D. A fractionating column for analysis of nucleic acids. Anal Biochem. 1960 Jun;1:66–77. doi: 10.1016/0003-2697(60)90020-8. [DOI] [PubMed] [Google Scholar]
  7. Shugart L., Novelli G. D., Stulberg M. P. Isolation and properties of undermethylated phenylalanine transfer ribonucleic acids from a relaxed mutant of Escherichia coli. Biochim Biophys Acta. 1968 Mar 18;157(1):83–90. doi: 10.1016/0005-2787(68)90266-9. [DOI] [PubMed] [Google Scholar]
  8. Weiss J. F., Kelmers A. D. A new chromatographic system for increased resolution of transfer ribonucleic acids. Biochemistry. 1967 Aug;6(8):2507–2513. doi: 10.1021/bi00860a030. [DOI] [PubMed] [Google Scholar]

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