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. 1970 Jun;102(3):716–721. doi: 10.1128/jb.102.3.716-721.1970

Genetic and Metabolic Controls for Sulfate Metabolism in Neurospora crassa: Isolation and Study of Chromate-Resistant and Sulfate Transport-Negative Mutants

George A Marzluf 1
PMCID: PMC247617  PMID: 5429722

Abstract

Mutants of Neurospora resistant to chromate were selected and all were found to map at a single genetic locus designated as cys-13. The chromate-resistant mutants grow at a wild-type rate on minimal media but are partially deficient in the transport of inorganic sulfate, especially during the conidial stage. An unlinked mutant, cys-14, is sensitive to chromate but transports sulfate during the mycelial stage at only 25% of the wild-type rate; cys-14 also grows at a fully wild-type rate on minimal media. The double-mutant strain, cys-13;cys-14, cannot utilize inorganic sulfate for growth and completely lacks the capacity to transport this anion. The only biochemical lesion that has been detected for the double-mutant strain is its loss in capacity for sulfate transport. Neurospora appears to possess two distinct sulfate permease species encoded by separate genetic loci. The transport system (permease I) encoded by cys-13 predominates in the conidial stage and is replaced by sulfate permease II, encoded by the cys-14 locus, during outgrowth into the mycelial phase. The relationship of these new mutants to cys-3, a regulatory gene that appears to control their expression, is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arst H. N., Jr Genetic analysis of the first steps of sulphate metabolism in Aspergillus nidulans. Nature. 1968 Jul 20;219(5151):268–270. doi: 10.1038/219268a0. [DOI] [PubMed] [Google Scholar]
  2. Bellenger N., Nissen P., Wood T. C., Segel I. H. Specificity and control of choline-O-sulfate transport in filamentous fungi. J Bacteriol. 1968 Nov;96(5):1574–1585. doi: 10.1128/jb.96.5.1574-1585.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dreyfuss J., Pardee A. B. Evidence for a sulfate-binding site external to the cell membrane of Salmonella typhimurium. Biochim Biophys Acta. 1965 Jun 15;104(1):308–310. doi: 10.1016/0304-4165(65)90256-4. [DOI] [PubMed] [Google Scholar]
  4. Jacobson E. S., Metzenberg R. L. A new gene which affects uptake of neutral and acidic amino acids in Neurospora crassa. Biochim Biophys Acta. 1968 Feb 1;156(1):140–147. doi: 10.1016/0304-4165(68)90113-x. [DOI] [PubMed] [Google Scholar]
  5. Kappy M. S., Metzenberg R. L. Multiple alterations in metabolite uptake in a mutant of Neurospora crassa. J Bacteriol. 1967 Nov;94(5):1629–1637. doi: 10.1128/jb.94.5.1629-1637.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lester G. Genetic control of amino acid permeability in Neurospora crassa. J Bacteriol. 1966 Feb;91(2):677–684. doi: 10.1128/jb.91.2.677-684.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Marzluf G. A., Metzenberg R. L. Positive control by the cys-3 locus in regulation of sulfur metabolism in Neurospora. J Mol Biol. 1968 Apr 28;33(2):423–437. doi: 10.1016/0022-2836(68)90199-x. [DOI] [PubMed] [Google Scholar]
  8. Metzenberg R. L., Parson J. W. Altered repression of some enzymes of sulfur utilization in a temperature-conditional lethal mutant of Neurospora. Proc Natl Acad Sci U S A. 1966 Mar;55(3):629–635. doi: 10.1073/pnas.55.3.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Pardee A. B. Crystallization of a sulfate-binding protein (permease) from Salmonella typhimurium. Science. 1967 Jun 23;156(3782):1627–1628. doi: 10.1126/science.156.3782.1627. [DOI] [PubMed] [Google Scholar]
  10. Pardee A. B., Prestidge L. S., Whipple M. B., Dreyfuss J. A binding site for sulfate and its relation to sulfate transport into Salmonella typhimurium. J Biol Chem. 1966 Sep 10;241(17):3962–3969. [PubMed] [Google Scholar]
  11. Pardee A. B. Purification and properties of a sulfate-binding protein from Salmonella typhimurium. J Biol Chem. 1966 Dec 25;241(24):5886–5892. [PubMed] [Google Scholar]
  12. Slayman C. W., Tatum E. L. Potassium transport in Neurospora. 3. Isolation of a transport mutant. Biochim Biophys Acta. 1965 Sep 27;109(1):184–193. doi: 10.1016/0926-6585(65)90102-0. [DOI] [PubMed] [Google Scholar]
  13. Stadler D. R. Genetic control of the uptake of amino acids in Neurospora. Genetics. 1966 Aug;54(2):677–685. doi: 10.1093/genetics/54.2.677. [DOI] [PMC free article] [PubMed] [Google Scholar]

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