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. 1989 Jan;63(1):226–232. doi: 10.1128/jvi.63.1.226-232.1989

Capped poly(A) leaders of variable lengths at the 5' ends of vaccinia virus late mRNAs.

B Y Ahn 1, B Moss 1
PMCID: PMC247676  PMID: 2462059

Abstract

Evidence for capped poly(A) leaders of variable lengths located immediately upstream of the translation initiation codon was obtained by direct analyses of a major late mRNA species. A decapping-recapping method was used to specifically substitute a radioactively labeled phosphate for an unlabeled one within the cap structure. RNase H-susceptible sites were made by hybridizing synthetic oligodeoxyribonucleotides to the mRNA encoding a late major structural protein of 11 kilodaltons. Sequences of the type m7G(5')pppAmp (Ap)nUpG. . ., where n varies from a few to more than 40 nucleotides, were deduced by analysis of the length and sequence of RNase H, RNase T1, and RNase U2 digestion products.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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